Bacillus haikouensis sp. nov., a facultatively anaerobic halotolerant bacterium isolated from a paddy soil

Abstract

A Gram-stain positive, rod-shaped, endospore-forming and facultatively anaerobic halotolerant bacterium, designated as C-89(T), was isolated from a paddy field soil in Haikou, Hainan Province, People's Republic of China. Optimal growth was observed at 37 °C and pH 7.0 in the presence of 4% NaCl (w/v). The predominant menaquinone was identified as MK-7, the major cellular fatty acids were identified as anteiso-C(15:0) and iso-C(15:0), and the major cellular polar lipids were identified as phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unknown phospholipids. The peptidoglycan type was determined to be based on meso-DAP. Based on 16S rRNA gene sequence similarity, the closest phylogenetic relatives were identified as Bacillus vietnamensis JCM 11124(T) (98.8% sequence similarity), Bacillus aquimaris JCM 11545(T) (98.6%) and Bacillus marisflavi JCM 11544(T) (98.5%). The DNA G+C content of strain C-89(T) was determined to be 45.4 mol%. The DNA-DNA relatedness values of strain C-89(T) with its closest relatives were below 18%. Therefore, on the basis of phylogenetic, chemotaxonomic, and phenotypic results, strain C-89(T) can be considered to represent a novel species within the genus Bacillus, for which the name Bacillus haikouensis sp. nov., is proposed. The type strain is C-89(T) (=KCTC 33545(T) = CCTCC AB 2014076(T)).

Endophytic Bacterial Isolates From Halophytes Demonstrate Phytopathogen Biocontrol and Plant Growth Promotion Under High Salinity

“…This species is a known endophytic bacterium (Han et al, 2011), shown to trigger an increase on monoterpenes, sesquiterpenes, tocopherols, and membrane sterols, compounds engaged in antioxidant capacity in leaf tissues of grape resulting in stress tolerance (Salomon et al, 2016). Bacillus haikouensis is halotolerant bacterium isolated from paddy soil, able to grow on up to 17% NaCl (Li et al, 2014).…”

“…This species is a known endophytic bacterium ( Han et al, 2011 ), shown to trigger an increase on monoterpenes, sesquiterpenes, tocopherols, and membrane sterols, compounds engaged in antioxidant capacity in leaf tissues of grape resulting in stress tolerance ( Salomon et al, 2016 ). Bacillus haikouensis is halotolerant bacterium isolated from paddy soil, able to grow on up to 17% NaCl ( Li et al, 2014 ). Oceanobacillus picturae is a halophilic phosphate-solubilizing species with demonstrated siderophore production potential, isolated from saline environments and shown to promote plant growth in mangroves and confer salinity stress tolerance in barley ( El-Tarabily and Youssef, 2010 ; Mapelli et al, 2013 ; Orhan and Demirci, 2020 ).…”

Halophytic bacterial endophytome: a potential source of beneficial microbes for a sustainable agriculture

“…This species is a known endophytic bacterium [44], that has been shown a systemic response that trigger an increase on monoterpenes, sesquiterpenes, tocopherols and membrane sterols, compounds engaged in antioxidant capacity in leaf tissues of grape resulting in stress tolerance [45]. Bacillus haikouensis is a recently described halotolerant bacterium isolated from paddy soil, able to grow on up to 17% NaCl concentrations [46]. Oceanobacillus picturae is a halophilic phosphate-solubilizing species with demonstrated siderophore production potential that has been isolated from saline environments and has been shown to promote plant growth promotion in mangroves and confer salinity stress tolerance in barley [47][48][49].…”

In Situ Discrimination and Cultivation of Active Degraders in Soils by Genome-Directed Cultivation Assisted by SIP-Raman-Activated Cell Sorting

“…(National Center for Biotechnology Information, Bethesda, MD, USA) and MEGA version 5.0 after multiple alignments. 56 Clustering was performed with the neighbor-joining method, and the phylogenetic tree was determined using bootstrap analysis based on 1200 resamplings. 13 C-DNA.…”