Bacterial 16S ribosomal DNA in house dust mite cultures

Valerio, C.; Murray, Patrick R.; Arlian, Larry G.; Slater, Jay E.

doi:10.1016/j.jaci.2005.09.046

Cited by 89 publications

(91 citation statements)

References 19 publications

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“…Or, mite extracts may include endotoxins that act through TLRs by stimulating them. Extracts of D. farinae and D. pteronyssinus have both been shown to contain endotoxins [22]. Another possibility is that a mite extract could induce the production of a single cytokine, which then induces the subsequent production of other cytokines in an autocrine fashion.…”

Section: Discussionmentioning

confidence: 99%

“…Extracts of house dust mites may also contain endotoxins[22], and these substances may influence the function of many cell types via the toll-like receptors (TLRs) on the cells. Other bioactive components of mite bodies and feces have not been characterized, and these could elicit their effects via pathways other than through PARs and TLRs.…”

Section: Introductionmentioning

confidence: 99%

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House Dust and Storage Mite Extracts Influence Skin Keratinocyte and Fibroblast Function

Arlian

Morgan

Peterson³

2007

Int Arch Allergy Immunol

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Background: The bodies of allergy-causing dust and storage mites likely contain many bioreactive molecules, including some that are allergenic. These molecules may penetrate the epidermis and dermis of the skin. However, little is known about the effects that most of the molecules from mites have on the function of cells in the skin, the overall inflammatory and immune reactions and the manifestation of allergic disease.The purpose of this research was to determine the response of cultured skin cells (keratinocytes and fibroblasts) to extracts of house dust and storage mites. Methods: Normal human epidermal keratinocytes and dermal fibroblasts were cultured with varying doses of extracts of the storage mites Acarus siro, Chortoglyphus arcuatus or Lepidoglyphus destructor or of the house dust mites Dermatophagoides farinae, D. pteronyssinus or Euroglyphus maynei in the absence or presence of lipopolysaccharide. Culture supernatants were collected 24 h later and assayed for the presence of various chemokines and cytokines. Results: Keratinocytes constitutively secreted interleukin (IL)-1 receptor antagonist/IL-1F3, growth-related oncogene α and transforming growth factor α, and these secretions were modulated by extracts of 1 or more of the mites tested. Mite extracts also modulated the production of IL-6, IL-8, monocyte chemoattractant protein 1, macrophage colony-stimulating factor and vascular endothelial growth factor from fibroblasts. Conclusions: The effects that mite extracts exerted on both keratinocytes and fibroblasts varied among the house dust mite species, among the storage mite species and between the house dust and storage mites. This study showed that extracts of mites contain substances that modulate the production of proinflammatory cytokines and chemokines secreted by normal human epidermal keratinocytes and dermal fibroblasts, and therefore may influence the course of pathophysiology in the skin in atopic dermatitis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

House Dust and Storage Mite Extracts Influence Skin Keratinocyte and Fibroblast Function

Arlian

Morgan

Peterson³

2007

Int Arch Allergy Immunol

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“…Whether these microbial compounds derived from endosymbionts and/or stable contaminants in mite cultures remains to be fully addressed. However, although the presence of large amounts of LPS and/or bacteria as well as β-glucans and/or fungi in house dust, the mites’ natural home environment, supports the microbial contamination hypothesis, bacterial 16S ribosomal DNA were identified in washed Dermatophagoides farinae or D. pteronyssinus as well as in sterilized poultry red mite [6,7]. These data suggest that endosymbiotic bacteria could also represent the source of contaminating LPS.…”

Section: Microbial Compounds In Hdm Extracts: Biocontaminants or Endomentioning

confidence: 99%

The Role of the House Dust Mite-Induced Innate Immunity in Development of Allergic Response

Jacquet

2010

Int Arch Allergy Immunol

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House dust mite (HDM) represents one of the most common sources of aeroallergens worldwide and more than 50% of allergic patients are sensitized to these allergenic molecules. HDM allergy research in the past has been mainly focused on adaptive, mite allergen-dependent immune responses. In recent years it has become clear that, although the allergen-specific CD4+ Th2 cellsorchestrate HDM allergic response,the innate immune system also plays a critical role in HDM-induced allergy pathogenesis. This review will summarize insights into diverse determinants that contribute to the HDM allergenicity through the activation of innate immunity. In addition to the capacity of mite allergens to directly activate mainly skin keratinocytes and airway epithelial cells, innate pattern recognition receptor ligands derived from HDM carriers are also involved in the development of allergic response by HDM.

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“…We believe that the majority of disadvantages that limit the broad applicability of SIT are related to the poor quality of allergen extracts [25][26][27][28][29][30][31][32]. SIT seems to be less effective for certain clinical manifestations of allergy such as asthma, food allergy and atopic dermatitis and for certain allergen sources such as house dust mites, moulds, cat, dog and food allergens [12].…”

Section: Factors Limiting the Broad Application Of Allergen-specific mentioning

confidence: 99%

Developments in allergen‐specific immunotherapy: from allergen extracts to allergy vaccines bypassing allergen‐specific immunoglobulin E and T cell reactivity

Focke

Swoboda

Marth

et al. 2010

Clin Experimental Allergy

101

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SummaryAllergen-specific immunotherapy (SIT) is the only specific and disease-modifying approach for the treatment of allergy but several disadvantages have limited its broad applicability. We argue that the majority of the possible disadvantages of SIT such as unwanted effects, poor efficacy and specificity as well as inconvenient application are related to the poor quality of natural allergen extracts, which are the active ingredients of all currently available allergy vaccines. Because of the progress made in the field of molecular allergen characterization, new allergy vaccines based on recombinant allergens, recombinant hypoallergenic allergen derivatives and allergen-derived T cell peptides have entered clinical testing and hold promise to reduce the side-effects and to increase the specificity as well as the efficacy of SIT. Here, we present a refined immunotherapy concept, which is based on the use of peptides derived from allergen surfaces that exhibit reduced, allergen-specific IgE as well as T cell reactivity. These peptides when fused to non-allergenic carriers give rise to allergen-specific protective IgG responses with T cell help from a non-allergenic carrier molecule. We summarize the experimental data demonstrating that such peptide vaccines can bypass allergen-specific IgE as well as T cell activation and may be administered at high doses without IgE-and T cell-mediated side-effects. Should these peptide vaccines prove efficacious and safe in clinical trials, it may become possible to develop convenient, safe and broadly applicable forms of SIT as true alternatives to symptomatic, drug-based allergy treatment.

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Bacterial 16S ribosomal DNA in house dust mite cultures

Cited by 89 publications

References 19 publications

House Dust and Storage Mite Extracts Influence Skin Keratinocyte and Fibroblast Function

House Dust and Storage Mite Extracts Influence Skin Keratinocyte and Fibroblast Function

The Role of the House Dust Mite-Induced Innate Immunity in Development of Allergic Response

Developments in allergen‐specific immunotherapy: from allergen extracts to allergy vaccines bypassing allergen‐specific immunoglobulin E and T cell reactivity

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