Bacterial alginate lyase: Enzymology, genetics and application

Murata, Kousaku; Inose, Tomoko; Hisano, Tomohiro; Abe, Shiro; Yonemoto, Yoshimasa; Yamashita, Tetsuo; Takagi, Masahiro; Sakaguchi, Kenji; Kimura, Akira; Imanaka, Tadayuki

doi:10.1016/0922-338x(93)90040-f

Cited by 107 publications

(46 citation statements)

References 32 publications

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“…Also, the use of probiotics can protect the host against infection, reduce harmful bacteria, and stimulate immune response. However, more researches should be done to show the accurate probiotic mechanism to apply them better for clinical purpose [8][9][10][11]. As we can see in the recent studies, microbiota like L. plantarum ZDY 2013 and L. fermentum UCO-979C have a great impact on the constraint of production of pro-inflammatory cytokines by H. pylori infection.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Probiotics on Immune System

Ganjbakhsh¹

2017

JBMOA

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In recent years, the research into probiotics indicates that supplementing with probiotics is probably more important than taking a multi-vitamin or antibiotics. This paper presents the interaction between host-derived probiotics and immunomodulatory. Studies have revealed probiotics can produce a unique substance that affects the expression of particular genes which are related to immune regulation in humans. The effect of gastric microbiota such as Lactobacillus fermentum and Lactobacillus plantarum is an amazing example to demonstrate how they are able to regulate the immune system. In fact, the studies indicate that L. plantarum ZDY 2013 pretreatment can prevent gastric mucosal inflammation induced by H. pylori. In addition, L. fermentum UCO-979C-obtained from human gut shows an efficient growth in the rodent (Mongolian gerbil). It can stop stimulation to produce cytokines like IL8 by H. pylori in gastric adenocarcinoma human cells (AGS)cells.

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Section: Resultsmentioning

confidence: 99%

The Effect of Probiotics on Immune System

Ganjbakhsh¹

2017

JBMOA

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“…Furthermore, in the case of strain A1, four kinds of alginate-depolymerizing enzymes (alginate lyases) are localized exclusively in the cytoplasm and are not secreted even into the periplasm. 17,26,27) Based on these observations, a novel pitdependent import system for macromolecules is considered to be present in strain A1 cells, but no systems for macromolecule import into microbial cells have been characterized so far. In order to understand the highly organized structure and function of the cell surface as well as the diverse metabolic abilities of strain A1, information on the whole genome sequence (genomics) and comprehensive analysis of proteins (proteomics) is required in addition to knowledge of the stereographical interaction of the functional units and three-dimensional structures of individual proteins.…”

Section: Pit-forming Bacteriummentioning

confidence: 99%

“…5A). 17,52) The three endotype alginate lyases originate from a single gene, 27,53) and release unsaturated di-, tri-, and tetrasaccharides from alginate.…”

Section: Polysaccharide Assimilation Alginate Metabolismmentioning

confidence: 99%

Structure and Function of Bacterial Super-Biosystem Responsible for Import and Depolymerization of Macromolecules

Hashimoto

Momma

Maruyama

et al. 2005

Bioscience, Biotechnology, and Biochemistry

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“…This enzyme has been found in brown algae [15,16], marine and soil bacteria [4,[17][18][19][20][21], herbivorous marine mollusks [22][23][24][25][26][27][28][29][30], and Chlorella virus [31]. According to the database for carbohydrate-active enzymes (CAZy, http://www.cazy.org), these alginate lyases have been classified under seven families, i.e., polysaccharide-lyase-family (PL) 5, 6, 7, 14, 15, 17, and 18.…”

Section: Introductionmentioning

confidence: 99%

Heat-stability and primary structure of the major alginate lyase isozyme LbAly35 from Littorina brevicula

2012

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Previously we isolated the major alginate lyase isozyme LbAly35 from a marine snail Littorina brevicula and showed that this enzyme was significantly heat-stable in a broad pH range compared with other molluscan alginate lyases (Hata et al., Fish. Sci. (2009) 75:755-763).LbAly35 showed practically no similarity to other molluscan alginate lyases in the N-terminal amino-acid sequence of 20 residues and no cross-reactivity with anti-abalone alginate lyase antiserum. These led us to consider that the primary structure of LbAly35 is considerably deviated from other molluscan enzymes. Thus, in the present study, we first compared the thermal stability of LbAly35 with an abalone alginate lyase, HdAly, and found that the first order inactivation rate constants for LbAly35 at 40 o C and 45 o C were 1/20 and 1/45 of those for HdAly, respectively. Then, we cloned cDNAs encoding LbAly35 and characterized its deduced amino-acid sequence comparing with those of other molluscan alginate lyases. The cDNAs were amplified by PCR and 5′-and 3′-RACE PCRs from the L. brevicula hepatopancreas cDNA using degenerated primers synthesized on the basis of partial amino-acid sequences of LbAly35.The cDNA covering entire translational region of LbAly35 comprised 1,093 bp and encoded an amino-acid sequence of 296 residues. The amino-acid sequence consisted of an initiation methionine, a putative signal peptide for secretion (22 residues), a propeptide-like region (10 residues), and a mature LbAly35 domain of 263 residues. Although the N-terminal region of LbAly35 was significantly deviated from those of other molluscan alginate lyases, the catalytic domain of LbAly35 showed ~45% identity to other molluscan enzymes which had been classified under polysaccharide-lyase-family-14 (PL-14). In addition, the amino-acid residues crucially important for the catalytic actions of PL-14 enzymes were also conserved in LbAly35.Accordingly, LbAly35 was regarded as a member of PL-14 as other molluscan alginate lyases despite of the significant deviation of its N-terminal region.

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Bacterial alginate lyase: Enzymology, genetics and application

Cited by 107 publications

References 32 publications

The Effect of Probiotics on Immune System

The Effect of Probiotics on Immune System

Structure and Function of Bacterial Super-Biosystem Responsible for Import and Depolymerization of Macromolecules

Heat-stability and primary structure of the major alginate lyase isozyme LbAly35 from Littorina brevicula

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