Bacterial and Candida albicans Adhesion on Different Root Canal Filling Materials and Sealers

Senges, Christian; Wrbas, Karl-Thomas; Altenburger, Markus Jörg; Follo, Marie; Spitzmüller, Bettina; Wittmer, Annette; Hellwig, Elmar; Al‐Ahmad, Ali

doi:10.1016/j.joen.2011.05.034

Cited by 28 publications

(21 citation statements)

References 56 publications

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“…AP exhibited superior sealing ability and cellular compatibility [44], [45] (although more bacteria tended to adhere [46]), and lower antimicrobial activity in comparison with AH and Grossman's sealer [40], [47]. Both AP and Grossman's sealers initiated lymphocytic and plasmocytic reactions [48].…”

Section: Discussionmentioning

confidence: 99%

Evaluation Physical Characteristics and Comparison Antimicrobial and Anti-Inflammation Potentials of Dental Root Canal Sealers Containing Hinokitiol In Vitro

Shih¹,

Lin

Chang

et al. 2014

PLoS ONE

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Hinokitiol displays potent antimicrobial activity. It has been used in toothpaste and oral-care gel to improve the oral lichen planus and reduce halitosis. The aim of this study was to evaluate the antimicrobial activity of 3 different dental root canal sealers with hinokitiol (sealers+H) and their physical and biological effects. AH Plus (epoxy amine resin-based, AH), Apexit Plus (calcium-hydroxide-based, AP), and Canals (zinc-oxide-eugenol-based, CA), were used in this study. The original AH and CA exhibited strong anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity, but AP did not. The setting time, working time, flowability, film thickness, and solubility of each sealer+0.2%H complied with ISO 6876:2001. CA+0.2%H exhibited high cytotoxicity, but the others sealers+0.2%H did not. Because hinokitiol combined with Zn2+ in CA creates a synergistic effect. The physical tests of AP+0.5%–1%H complied with ISO 6876:2001, improved antimicrobial activity, inhibited inflammation genes cyclooxygenase-2 (COX-2) and hypoxia-inducible factor-1α (HIF-1α) mRNA in MG-63 cells and human gingival fibroblasts (HGF), and down-regulated lysyl oxidase (LOX) mRNA of HGF. In summary, AH and CA demonstrated strong antimicrobial activity, but AP did not. Application of hinokitiol increases AH anti-MRSA activity should less than 0.2% for keep well flowability. AP+0.5%–1% hinokitiol exhibited strong physical, antibacterial, and anti-inflammation potentials, and inhibited S. aureus abscess formation. Applying an appreciable proportion of hinokitiol to epoxy-amine-resin-based and calcium-hydroxide-based root canal sealers is warranted, but the enhanced cytotoxicity and synergistic effect must be considered.

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Section: Discussionmentioning

confidence: 99%

Evaluation Physical Characteristics and Comparison Antimicrobial and Anti-Inflammation Potentials of Dental Root Canal Sealers Containing Hinokitiol In Vitro

Shih¹,

Lin

Chang

et al. 2014

PLoS ONE

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“…Moreover, endodontic microorganisms have been shown to have a high affinity for root canal filling materials, especially for gutta-percha. Because of the substantial bacterial adhesion, subsequent biofilm formation can lead to the persistency of the microorganisms in the root canals [17].…”

Section: Introductionmentioning

confidence: 99%

Rapid Kill—Novel Endodontic Sealer and Enterococcus faecalis

et al. 2013

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With growing concern over bacterial resistance, the identification of new antimicrobial means is paramount. In the oral cavity microorganisms are essential to the development of periradicular diseases and are the major causative factors associated with endodontic treatment failure. As quaternary ammonium compounds have the ability to kill a wide array of bacteria through electrostatic interactions with multiple anionic targets on the bacterial surface, it is likely that they can overcome bacterial resistance. Melding these ideas, we investigated the potency of a novel endodontic sealer in limiting Enterococcus faecalis growth. We used a polyethyleneimine scaffold to synthesize nano-sized particles, optimized for incorporation into an epoxy-based endodontic sealer. The novel endodontic sealer was tested for its antimicrobial efficacy and evaluated for biocompatibility and physical eligibility. Our results show that the novel sealer foundation affixes the nanoparticles, achieving surface bactericidal properties, but at the same time impeding nanoparticle penetration into eukaryotic cells and thereby mitigating a possible toxic effect. Moreover, adequate physical properties are maintained. The nanosized quaternary amine particles interact within minutes with bacteria, triggering cell death across wide pH values. Throughout this study we demonstrate a new antibacterial perspective for endodontic sealers; a novel antibacterial, effective and safe antimicrobial means.

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“…E. faecalis, Streptococcus mutans, Streptococcus sanguinis, Candida albicans, and Prevotella nigrescens are able to adhere to gutta-percha or endodontic sealers (8), allowing biofilm formation (8)(9)(10)(11).…”

mentioning

confidence: 99%

“…On the other hand, confocal laser scanning microscopy has been used (9,15) for analysis of multiple sections of the biofilm at different focal planes, with ease of observation and preparation of samples. Use of reactive dye kits, such as Live/Dead (Molecular Probes, Inc, Eugene, OR), and of imaging software allow identification and quantification of viable and nonviable cells (12,15,19).…”

mentioning

confidence: 99%

Comparative Analysis of Enterococcus faecalis Biofilm Formation on Different Substrates

Guerreiro-Tanomaru

Faria-Júnior

Duarte

et al. 2013

Journal of Endodontics

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Introduction: The aim of this study was to compare Enterococcus faecalis biofilm formation on different substrates. Methods: Cell culture plates containing growth medium and E. faecalis (ATCC 29212) were used to grow biofilm on bovine dentin, gutta-percha, hydroxyapatite, or bovine bone. Substrates were incubated at 37 C for 14 or 21 days, and the medium was changed every 48 hours. After the growth induction periods, specimens (n = 5 per group and per induction period) were stained by using Live/Dead, and the images were analyzed under a confocal microscope. The total biovolume (mm 3 ), live bacteria biovolume (mm 3 ), and substrate coverage (%) were quantified by using the BioImage_L software. Results obtained were analyzed by nonparametric tests (P = .05). Results: Biofilm formation was observed in all groups. Gutta-percha had the lowest total biovolume at 14 days (P < .05) and hydroxyapatite the highest at 21 days (P < .05). No significant difference was observed in green biovolume at 14 days. At 21 days, however, hydroxyapatite had the highest volume (P < .05). The percentages of coverage were similar among all substrates at 21 days (P > .05), but at 14 days, bovine bone presented the highest coverage (P < .05). Conclusions: E. faecalis was capable of forming biofilm on all substrates during both growth periods; hydroxyapatite presented the highest rates of biofilm formation. The type of substrate influenced the biofilm characteristics, according to the parameters evaluated. (J Endod 2013;39:346-350)

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Bacterial and Candida albicans Adhesion on Different Root Canal Filling Materials and Sealers

Cited by 28 publications

References 56 publications

Evaluation Physical Characteristics and Comparison Antimicrobial and Anti-Inflammation Potentials of Dental Root Canal Sealers Containing Hinokitiol In Vitro

Evaluation Physical Characteristics and Comparison Antimicrobial and Anti-Inflammation Potentials of Dental Root Canal Sealers Containing Hinokitiol In Vitro

Rapid Kill—Novel Endodontic Sealer and Enterococcus faecalis

Comparative Analysis of Enterococcus faecalis Biofilm Formation on Different Substrates

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