Bacterial and fungal communities within and among geographic samples of the hemp pest Psylliodes attenuata from China

Guo, Long; Tang, Chao; Gao, Chengde; Li, Zhimin; Cheng, Yi; Chen, Jia; Wang, Tuhong; Xu, Jianping

doi:10.3389/fmicb.2022.964735

Cited by 3 publications

(3 citation statements)

References 50 publications

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“…However, the reason for its isolation from beetles only in Khumaga is unclear (Supporting Information: Figure and Table ). Cutaneotrichosporon species have been reported as the most common basidiomycetous genera from hemp flea beetles (Guo et al, 2022). The ecological role of C. arboriforme beyond its association with dung beetles is not clear.…”

Section: Resultsmentioning

confidence: 99%

Diversity of dung beetle‐associated yeasts from pristine environments of Botswana

Nwaefuna

Boekhout

Vrhovšek

et al. 2023

Yeast

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Yeast-insect interactions are increasingly becoming an attractive source of discovery for previously unknown, unique, diverse, and industrially relevant yeast species. Despite a wealth of studies that have recently focused on yeasts in symbiotic association with Hymenopteran insects, yeasts associated with Coleopteran insects, such as lignocellulosic-rich dung-dependent beetles, remain poorly studied. Trends in yeast discovery suggest that species richness and diversity can be attributed to the ecological niche of the insect. Here, we considered the potential of dung beetles inhabiting the extreme environments of Botswana, characterized by desert-like conditions (semi-arid to arid and hot) as well as protected pristine environments, as possible attribute niches that can shape the extremophilic and diverse life history strategies of yeasts. We obtained a total of 97 phylogenetically diverse yeast isolates from six species of dung beetles from Botswana's unexplored environments, representing 19 species belonging to 11 genera. The findings suggest that the guts of dung beetles are a rich niche for non-Saccharomyces yeast species.Meyerozyma and Pichia were the most dominant genera associated with dung beetles, representing 55% (53 out of 97) of the yeast isolates in our study.Trichosporon and Cutaneotrichosporon genera represented 32% (31 out of 97) of the isolates. The remaining isolates belonged to Apiotrichum, Candida, Diutina, Naganishia, Rhodotorula, and Wickerhamiella genera (12 out of 97). We found out that about 62% (60 out of 97) of the isolates were potentially new species because of their low internal transcribed spacer (ITS) sequence similarity when compared to the most recent optimal species delineation threshold. A single isolate was unidentifiable using the ITS sequences. Using an in silico polymerase chain reaction-restriction fragment length polymorphism approach, we revealed that there was genetic diversity within isolates of the same species. Our results contribute to the knowledge and understanding of the diversity of dung beetleassociated yeasts.

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Section: Resultsmentioning

confidence: 99%

Diversity of dung beetle‐associated yeasts from pristine environments of Botswana

Nwaefuna

Boekhout

Vrhovšek

et al. 2023

Yeast

View full text Add to dashboard Cite

show abstract

“…The primers for the fungal DNA amplification were ITS1F (5 -CTTGGTCATTTAGAGGAAGTAA-3 ) and ITS2R (5 -GCTGCGTT CTTCATCGATGC-3 ). The PCR amplification, the Illumina MiSeq sequencing, and the analysis of sequencing data were performed following the procedures described in our previous study [14]. The raw reads have been deposited in the NCBI Sequence Read Archive (SRA) database (BioProject Number: PRJNA957946; sequence accession numbers SRR24235724-SRR24235735).…”

Section: Amplicon Sequencingmentioning

confidence: 99%

“…As a result, the insect microbiota has drawn increasing attention from the scientific community [13]. The insect microbiota can detoxify toxic secondary metabolites and xenobiotics, for example, nicotine, terpenes, and insecticides [14]. The microbiota can also generate and transmit signals among hosts; protect hosts against pathogens; and modify host immunity and behavior [15].…”

Section: Introductionmentioning

confidence: 99%

Effects of Cadmium Stress on Bacterial and Fungal Communities in the Whitefly Bemisia tabaci

Guo,

Li,

2023

IJMS

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Heavy metal contamination is among the most prominent environmental problems in China, posing serious threats to both ecosystem and human health. Among the diverse heavy metal contaminants, cadmium is the most serious. The whitefly Bemisia tabaci is a cosmopolitan pest capable of causing severe damage to a broad range of agricultural crops, especially vegetables. At present, little is known about the effects of cadmium stress on B. tabaci, including on its bacterial and fungal communities. In the current study, we investigated the effects of cadmium on bacterial and fungal communities in whiteflies. Meta-barcode sequencing of the 16S rRNA gene revealed that the whitefly bacterial community contained 264 operational taxonomic units (OTUs) belonging to 201 known genera and 245 known species. The top five most frequent bacterial genera were Rickettsia, Rhodococcus, Candidatus Portiera, Candidatus Hamiltonella, and Achromobacter. Meta-barcode sequencing of the fungal ITS locus revealed that the whitefly fungal community contained 357 OTUs belonging to 187 known genera and 248 known species. The top five most frequent fungal genera were Wallemia, unclassified_f_Dipodascaceae, Apiotrichum, Penicillium, and unclassified_o_Saccharomycetales. Cadmium exposure reduced the fungal OTU richness but increased the bacterial Shannon and Simpson diversity indices in whiteflies. In addition, upon exposure to cadmium, the microbial community composition in whiteflies changed significantly, with increased prevalence of the bacterial genera Rhodococcus and Exiguobacterium and fungal genus Wallemia. Our results indicate that the whitefly microbiota likely contributed to their adaptation and resistance to cadmium and suggested that whiteflies may contain microbes that could help remediate cadmium contamination in natural environments and agricultural fields.

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Cladosporium—Insect Relationships

Nicoletti,

Russo,

Becchimanzi

2024

JoF

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The range of interactions between Cladosporium, a ubiquitous fungal genus, and insects, a class including about 60% of the animal species, is extremely diverse. The broad case history of antagonism and mutualism connecting Cladosporium and insects is reviewed in this paper based on the examination of the available literature. Certain strains establish direct interactions with pests or beneficial insects or indirectly influence them through their endophytic development in plants. Entomopathogenicity is often connected to the production of toxic secondary metabolites, although there is a case where these compounds have been reported to favor pollinator attraction, suggesting an important role in angiosperm reproduction. Other relationships include mycophagy, which, on the other hand, may reflect an ecological advantage for these extremely adaptable fungi using insects as carriers for spreading in the environment. Several Cladosporium species colonize insect structures, such as galleries of ambrosia beetles, leaf rolls of attelabid weevils and galls formed by cecidomyid midges, playing a still uncertain symbiotic role. Finally, the occurrence of Cladosporium in the gut of several insect species has intriguing implications for pest management, also considering that some strains have proven to be able to degrade insecticides. These interactions especially deserve further investigation to understand the impact of these fungi on pest control measures and strategies to preserve beneficial insects.

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Bacterial and fungal communities within and among geographic samples of the hemp pest Psylliodes attenuata from China

Cited by 3 publications

References 50 publications

Diversity of dung beetle‐associated yeasts from pristine environments of Botswana

Diversity of dung beetle‐associated yeasts from pristine environments of Botswana

Effects of Cadmium Stress on Bacterial and Fungal Communities in the Whitefly Bemisia tabaci

Cladosporium—Insect Relationships

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