Bacterial and Fungal Community Composition and Functional Activity Associated with Lake Wetland Water Level Gradients

Ma, Yi; Li, Jinqian; Wu, Juan; Kong, Zhaochen; Feinstein, Larry M.; Ding, Xia; Ge, Gang; Wu, Lang

doi:10.1038/s41598-018-19153-z

Cited by 51 publications

(33 citation statements)

References 59 publications

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“…B). The significant influence of water level on soil physiochemical properties and microbial community structure of non‐hydrocarbon‐impacted forest and freshwater wetland soils have been previously demonstrated (Brockett et al ., ; Ma et al ., ). Thus, the selective presence of hydrocarbon waste, combined presence of standing water and lack of tillage at depths below 10 cm contribute to landfarm community structure.…”

Section: Discussionmentioning

confidence: 99%

Spatial analysis of a hydrocarbon waste‐remediating landfarm demonstrates influence of management practices on bacterial and fungal community structure

Bergsveinson

Perry

Simpson

et al. 2019

Microbial Biotechnology

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Summary Cultivation of dedicated soil plots called ‘landfarms' is an effective technology for bioremediation of hydrocarbon waste generated by various industrial practices. To understand the influence of soil conditions on landfarm microbial communities, analysis of bacterial and fungal community structure using next‐generation sequencing at different sections and depths was performed across a hydrocarbon‐waste landfarm in Regina, Saskatchewan, Canada. While a core set of hydrocarbon‐associated bacterial and fungal taxa are present throughout the landfarm, unique bacterial and fungal operational taxonomic units are differentially abundant at sections within the landfarm, which correlate with differences in soil physiochemical properties and management practices. Increased frequency of waste application resulted in strong positive correlations between bacterial community assemblages and elevated amounts of oil, grease and F3 – F4 hydrocarbon fractions. In areas of standing water and lower application of hydrocarbon, microbial community structure correlated with soil pH, trace nutrients and metals. Overall, diversity and structure of bacterial communities remain relatively stable across the landfarm, while in contrast, fungal community structure appears more responsive to soil oxygen conditions. Results are consistent with the hypothesis that years of bioremediation activity have shaped microbial communities; however, several management practices can be undertaken to increase efficiency of remediation, including the removal of standing water and soil tilling across the landfarm.

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Section: Discussionmentioning

confidence: 99%

Spatial analysis of a hydrocarbon waste‐remediating landfarm demonstrates influence of management practices on bacterial and fungal community structure

Bergsveinson

Perry

Simpson

et al. 2019

Microbial Biotechnology

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“…CLPP is a useful tool for assessing microbial community metabolic function and functional diversity in both terrestrial and aquatic environments [41][42][43]. CLPP, also known as carbon source utilization patterns of soil microbial communities, is assessed using a Biolog-ECO micro-plate (Category NO.…”

Section: Community Level Physiological Profilingmentioning

confidence: 99%

The Effects of Different Lead Pollution Levels on Soil Microbial Quantities and Metabolic Function with/without Salix integra Thunb. Planting

Niu

Zhou

et al. 2019

Forests

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Background and Objectives: Salix integra Thunb., a fast-growing woody species, has been used in phytoremediation in recent years. It has the potential to accumulate high amounts of lead (Pb) in its growth, however, its effects on soil microbial community structure and function during its phytoextraction processes are not well understood, especially at different pollution levels. Materials and Methods: In our study, we set unplanted and planted Salix integra in areas with four levels of Pb treatments (0, 500, 1000, and 1500 mg/kg). After six months of planting, the rhizospheric soil, bulk soil, and unplanted soil were collected. Soil properties and microbes participating in nitrogen and phosphorus cycling were measured, following standard methods. Microbial metabolic functions were assessed using a Biolog-ECO microplate. Results: The bacteria (nitrogen-fixing bacteria, ammonifying bacteria, inorganic phosphorus-solubilizing bacteria, and nitrosobacteria) all increased in the 500 mg/kg treatment and decreased in the 1500 mg/kg treatment compared with the 0 mg/kg treatment, especially in rhizospheric soil. The microbial metabolisms decreased along with the increase of Pb levels, with the exception of the rhizospheric soil with a 500 mg/kg treatment. The metabolic patterns were relative to the pollution levels. The utilization of carbohydrates was decreased, and of amino acids or fatty acids was increased, in the 500 mg/kg treatment, while the opposite occurred in the 1500 mg/kg treatment. The values of soil properties, microbial quantities, and metabolic activities were higher in rhizospheric than bulk soil, while the differences between bulk and unplanted soil were different among the different Pb treatments. The soil properties had little effect on the microbial quantities and metabolic activities. Conclusions: S. integra planting and Pb levels had an interactive effect on the microbial community. In general, S. integra planting promoted microbial quantities and metabolic activity in rhizospheric soil. Lower Pb pollution increased microbial quantities and promoted the utilization of amino acids or fatty acids, while higher Pb concentrations decreased microbial quantities and metabolic activities, and promoted the utilization of carbohydrates.

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“…Previous studies have suggested that water level fluctuations could regulate the transformation of nutrients by changing bacterial activities in active erosion and transport zones 17,[20][21][22][23][24] . In seasonal lakes, drying and inundation cycles within a year can influence nitrogen cycling by regulating nitrogen metabolism pathways [25][26][27] , such as anammox, nitrification, denitrification, and nitrogen fixation [28][29][30] .Determining metabolic functions related to the nitrogen cycle of the microbial communities in seasonal lakes is essential for understanding their roles in biogeochemical processes related to nutrient cycles 2,3,31,32 and their response to water level fluctuations 24,27,33,34 . Previous study indicated that bacterial communities are distinct taxonomically and functionally in the dry-season and wet-season in Poyang Lake 24 .…”

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confidence: 99%

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confidence: 99%

“…Determining metabolic functions related to the nitrogen cycle of the microbial communities in seasonal lakes is essential for understanding their roles in biogeochemical processes related to nutrient cycles 2,3,31,32 and their response to water level fluctuations 24,27,33,34 . Previous study indicated that bacterial communities are distinct taxonomically and functionally in the dry-season and wet-season in Poyang Lake 24 .…”

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confidence: 99%

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Microbial community structure and functional properties in permanently and seasonally flooded areas in Poyang Lake

Liu

Ren

et al. 2020

Sci Rep

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Water level fluctuations are an inherent feature regulating the ecological structures and functions of lakes. It is vital to understand the effects of water level fluctuations on bacterial communities and metabolic characteristics in freshwater lakes in a changing world. However, information on the microbial community structure and functional properties in permanently and seasonally flooded areas are lacking. Poyang Lake is a typical seasonal lake linked to the Yangtze River and is significantly affected by water level fluctuations. Bottom water was collected from 12 sampling sites: seven inundated for the whole year (inundated areas) and five drained during the dry season (emerged areas). High-throughput 16S rRNA gene sequencing was used to identify the bacterial communities. The results showed that the taxonomic structure and potential functions of the bacterial communities were significantly different between the inundated and emerged areas. Cyanobacteria was dominant in both areas, but the relative abundance of Cyanobacteria was much higher in the emerged areas than in the inundated areas. Bacterial communities were taxonomically sensitive in the inundated areas and functionally sensitive in the emerged areas. Nitrogen, phosphorus, and dissolved organic carbon concentrations and their ratios, as well as dissolved oxygen, played important roles in promoting the bacterial taxonomic and functional compositional patterns in both areas. According to the metabolic predictions based on 16S rRNA gene sequences, the relative abundance of functional genes related to assimilatory nitrate reduction in the emerged areas was higher than in the inundated areas, and the relative abundance of functional genes related to dissimilatory nitrate reduction in the inundated areas was higher. These differences might have been caused by the nitrogen differences between the permanently and seasonally flooded areas caused by intra-annual water level fluctuations. The relative abundance of functional genes associated with denitrification was not significantly different in the inundated and emerged areas. This study improved our knowledge of bacterial community structure and nitrogen metabolic processes in permanently and seasonally flooded areas caused by water level fluctuations in a seasonal lake.Microbial assemblages are fundamental components of aquatic ecosystems and play important roles in driving global energy fluxes and biogeochemical cycling 1 . Microbial communities have high species diversity and genetic diversity 2-5 , which make them sensitive to environmental perturbations 6,7 . The relative balance of external nutrient loading influences microbial functional genes and associated metabolic processes in lake ecosystems 8 . Understanding the taxonomic and functional compositions of microbial communities is essential to elucidating the responses of natural bacterial communities to environmental perturbations, such as hydrological fluctuations and nutrient pollution 9-11 . Poyang Lake (PYL) is a seasonal lake characterized by ...

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Bacterial and Fungal Community Composition and Functional Activity Associated with Lake Wetland Water Level Gradients

Cited by 51 publications

References 59 publications

Spatial analysis of a hydrocarbon waste‐remediating landfarm demonstrates influence of management practices on bacterial and fungal community structure

Spatial analysis of a hydrocarbon waste‐remediating landfarm demonstrates influence of management practices on bacterial and fungal community structure

The Effects of Different Lead Pollution Levels on Soil Microbial Quantities and Metabolic Function with/without Salix integra Thunb. Planting

Microbial community structure and functional properties in permanently and seasonally flooded areas in Poyang Lake

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