Bacterial and fungal diversity in the gut of polystyrene-fed Alphitobius diaperinus (Insecta: Coleoptera)

Cucini, Claudio; Leo, Chiara; Vitale, Matteo; Frati, Francesco; Carapelli, Antonio; Nardi, Francesco

doi:10.1016/j.angen.2020.200109

Cited by 21 publications

(16 citation statements)

References 49 publications

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“…To train the classifier artifacts for taxonomic attribution of 16S sequences, the SILVA 132 database was employed (Quast et al 2013), using feature-classifier plugin (Bokulich et al 2018) [Protocol S1]. Resulting sequences were matched with those of Cucini et al (2020) dataset though the NCBI BLAST+ tools (Camacho et al 2009). Taxonomic distribution results were visualized QIIME2 view and also compared with those of the first 16S rRNA survey on PS-fed A. diaperinus larvae (Cucini et al 2020).…”

Section: Sequencing Of 16s Rrna Ampliconsmentioning

confidence: 99%

“…Resulting sequences were matched with those of Cucini et al (2020) dataset though the NCBI BLAST+ tools (Camacho et al 2009). Taxonomic distribution results were visualized QIIME2 view and also compared with those of the first 16S rRNA survey on PS-fed A. diaperinus larvae (Cucini et al 2020). Raw sequencing reads were deposited in the Sequence Read Archive (SRA, NCBI) with the Bio-Project ID PRJNA781815.…”

Section: Sequencing Of 16s Rrna Ampliconsmentioning

confidence: 99%

“…1). From the initial 16S rRNA survey on A. diaperinus larval microbiome reported by Cucini et al (2020), a total amount of 295 different OTUs were retrieved with only six as differentially abundant in PS group, namely: two Pseudomonas sp., Cronobacter sp., Kocuria sp., Pseudogracilibacillus sp. and Virgibacillus sp..…”

Section: Sequencing Of 16s Rrna Ampliconsmentioning

confidence: 99%

“…A recent study carried out on another coleopteran species, Alphitobius diaperinus, concluded that also this beetle is capable of eating and degrading PS. In this species, comparative analyses of the gut microbiome have revealed several microbial taxa to be differentially abundant between PS-fed larvae and control groups, thus providing an initial list of microbial candidates involved in the degradation process (Cucini et al 2020). The lesser mealworm A. diaperinus is a nocturnal cosmopolitan Tenebrionidae, commonly found in litter, manure and chicken coops.…”

Section: Introductionmentioning

confidence: 99%

“…The aim of this study was to further expand the work started by Cucini et al (2020) focusing on the gut-associated bacterial communities of PS-fed larvae of A. diaperinus. An enrichment procedure in liquid carbon-free basal medium with PS films as sole carbon source starting from PS-fed larvae of A. diaperinus was performed and analysed in order to (i) compare the composition of enriched communities by culture-dependent and culture-independent molecular methods and to (ii) obtain a collection of bacteria potentially exploitable for synthetic microbiota application.…”

Section: Introductionmentioning

confidence: 99%

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Polystyrene shaping effect on the enriched bacterial community from the plastic-eating Alphitobius diaperinus (Insecta: Coleoptera)

et al. 2022

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Plastic pollution has become a serious issue of global concern, and biodegradation of plastic wastes is representing one attractive environment-friendly alternative to traditional disposal paths. It is known that insects are involved in the plastic polymer degradation process, with reported evidence of tenebrionid beetle larvae capable to degrade polystyrene (PS), one of the most used plastics worldwide. Recently, a ribosomal RNA based survey on the insect gut microbiota of the lesser mealworm Alphitobius diaperinus has revealed differentially abundant microbial taxa between PS-fed larvae and control group. Following these findings, an enrichment bacterial culture was set up in liquid carbon-free basal medium with PS film as sole carbon source using PS-fed larvae of A. diaperinus as inoculum. After two-months the culture was analysed both by molecular and culture-based methods. Isolated bacteria which had become prevalent under the selective enrichment conditions resulted ascribable to three taxonomic groups: Klebsiella, Pseudomonas, and Stenothrophomonas. The predominance of these groups in PS-fed larvae was confirmed by using bacterial 16S rRNA gene amplicon sequencing, and it was consistent with the results of previous reports. Isolated bacteria were able to attach to PS surfaces and SEM observations showed the presence of thin fibrillar structures connecting the bacterial cells to the abiotic surface.

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