Bacterial Copper Resistance and Virulence

Pontel, Lucas B.; Checa, Susana K.; Soncini, Fernando C.

doi:10.1007/978-3-319-18570-5_1

Cited by 9 publications

(11 citation statements)

References 117 publications

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“…As shown in Fig. 5B, addition of Cu increased transcription from the PcpxP promoter in the wild-type strain, as expected for a Cpx-regulated gene (1,(16)(17)(18). Remarkably, in the absence of a functional scsABCD locus, Cu-mediated cpxP induction showed a significant increase ( Fig.…”

Section: Role Of the Scs Proteins In Response To H 2 Osupporting

confidence: 75%

CpxR/CpxA ControlsscsABCDTranscription To Counteract Copper and Oxidative Stress in Salmonella enterica Serovar Typhimurium

2018

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Periplasmic thiol/disulfide oxidoreductases participate in the formation and isomerization of disulfide bonds and contribute to the virulence of pathogenic microorganisms. Among the systems encoded in the genome, the system encoded by the locus was shown to be required to cope with Cu and HO stress. Here we report that this locus forms an operon whose transcription is driven by a promoter upstream of and depends on CpxR/CpxA and on Cu. Furthermore, genes homologous to, , and are always detected immediately downstream of and in the same genetic arrangement in all-harboring enterobacterial species. Also, a CpxR-binding site is detected upstream of in most of those species, providing evidence of evolutionarily conserved function and regulation. Each individual gene shows a different role in copper and/or HO resistance, indicating hierarchical contributions of these factors in the defense against these intoxicants. A protective effect of Cu preincubation against HO toxicity and the increased Cu-mediated activation of in the Δ mutant suggest that the CpxR/CpxA-controlled transcription of the ScsABCD system contributes to prevent Cu toxicity and to restore the redox balance at the envelope. Copper intoxication triggers both specific and nonspecific responses in The locus, which codes for periplasmic thiol/disulfide-oxidoreductase/isomerase-like proteins, has been the focus of attention because it is necessary for copper resistance, oxidative stress responses, and virulence and because it is not present in nonpathogenic Still, the conditions under which the locus is expressed and the roles of its individual components remain unknown. In this report, we examine the contribution of each Scs factor to survival under HO and copper stress. We establish that the genes form a copper-activated operon controlled by the CpxR/CpxA signal transduction system, and we provide evidence of its conserved gene arrangement and regulation in other bacterial pathogens.

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Section: Role Of the Scs Proteins In Response To H 2 Osupporting

confidence: 75%

CpxR/CpxA ControlsscsABCDTranscription To Counteract Copper and Oxidative Stress in Salmonella enterica Serovar Typhimurium

2018

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“…Copper is an essential trace element for most organisms as well as microorganisms. However, it can be harmful and toxic even at low levels . To adapt to a copper toxic environment, bacteria have evolved specific copper homeostasis systems and diverse strategies to maintain a suitable intracellular concentration of this essential metal .…”

Section: Discussionmentioning

confidence: 99%

Responses to copper stress in the metal‐resistant bacterium Cupriavidus gilardii CR3: a whole‐transcriptome analysis

Huang

Mao

et al. 2019

J Basic Microbiol

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Microbial metal-resistance mechanisms are the basis for the application of microorganisms in metal bioremediation. Despite the available studies of bacterial molecular mechanisms to resistance metals ions (particularly copper), the understanding of bacterial metal resistance is very limited from the transcriptome perspective. Here, responses of the transcriptome (RNA-Seq) was investigated in Cupriavidus gilardii CR3 exposed to 0.5 mM copper, because strain CR3 had a bioremoval capacity of 38.5% for 0.5 mM copper. More than 24 million clean reads were obtained from six libraries and were aligned against the C. gilardii CR3 genome. A total of 310 genes in strain CR3 were significantly differentially expressed under copper stress. Apart from the routine copper resistance operons cus and cop known in previous studies, Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses of differentially expressed genes indicated that the adenosine triphosphate-binding cassette transporter, amino acid metabolism, and negative chemotaxis collectively contribute to the copper-resistant process. More interestingly, we found that the genes associated with the type III secretion system were induced under copper stress. No such results were reordered in bacteria to date. Overall, this comprehensive network of copper responses is useful for further studies of the molecular mechanisms underlying responses to copper stress in bacteria. K E Y W O R D Sbacteria, copper, Cupriavidus, RNA-Seq

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“…The essential as well as toxic nature of copper makes its active handling a vital skill for most organisms (6,11). Recently reported evidence indicates that intracellular copper actively contributes to the host innate immune response against bacterial infections, and that pathogens have acquired specific traits to deal with this intoxicant (29)(30)(31).…”

Section: Discussionmentioning

confidence: 99%

Compartment and signal-specific codependence in the transcriptional control of Salmonella periplasmic copper homeostasis

Pezza

Pontel

López

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Copper homeostasis is essential for bacterial pathogen fitness and infection, and has been the focus of a number of recent studies. In Salmonella, envelope protection against copper overload and macrophage survival depends on CueP, a major copper-binding protein in the periplasm. This protein is also required to deliver the metal ion to the Cu/Zn superoxide dismutase SodCII. The Salmonella-specific CueP-coding gene was originally identified as part of the Cue regulon under the transcriptional control of the cytoplasmic copper sensor CueR, but its expression differs from the rest of CueR-regulated genes. Here we show that cueP expression is controlled by the concerted action of CueR, which detects the presence of copper in the cytoplasm, and by CpxR/CpxA, which monitors envelope stress. Copper-activated CueR is necessary for the appropriate spatial arrangement of the −10 and −35 elements of the cueP promoter, and CpxR is essential to recruit the RNA polymerase. The integration of two ancestral sensory systems—CueR, which provides signal specificity, and CpxR/CpxA, which detects stress in the bacterial envelope—restricts the expression of this periplasmic copper resistance protein solely to cells encountering surplus copper that disturbs envelope homeostasis, emulating the role of the CusR/CusS regulatory system present in other enteric bacteria.

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Bacterial Copper Resistance and Virulence

Cited by 9 publications

References 117 publications

CpxR/CpxA ControlsscsABCDTranscription To Counteract Copper and Oxidative Stress in Salmonella enterica Serovar Typhimurium

CpxR/CpxA ControlsscsABCDTranscription To Counteract Copper and Oxidative Stress in Salmonella enterica Serovar Typhimurium

Responses to copper stress in the metal‐resistant bacterium Cupriavidus gilardii CR3: a whole‐transcriptome analysis

Compartment and signal-specific codependence in the transcriptional control of Salmonella periplasmic copper homeostasis

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