Bacterial diversity in persistent periapical lesions on root-filled teeth

Handal, Trude; Caugant, Dominique A.; Olsen, Ingar; Sunde, Pia Titterud

doi:10.3402/jom.v1i0.1946

Cited by 29 publications

(21 citation statements)

References 51 publications

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“…The only report using a cloning and sequencing method was the study by Handal et al . (), which excluded lesions with the presence of a sinus tract to eliminate possible communication with the oral cavity. They detected bacteria in 17 of 20 samples, whilst examining fewer clones in total (267 clones).…”

Section: Discussionmentioning

confidence: 99%

Microbial community in persistent apical periodontitis: a 16S rRNA gene clone library analysis

et al. 2014

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Section: Discussionmentioning

confidence: 99%

Microbial community in persistent apical periodontitis: a 16S rRNA gene clone library analysis

et al. 2014

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“…Except for acute and chronic apical abscesses, it is still controversial whether asymptomatic apical periodontitis lesions can harbor bacteria for very long beyond the initial tissue invasion . Studies using culture‐dependent or culture‐independent molecular biology methods have reported the extraradicular occurrence of a complex microbiota associated with apical periodontitis lesions that does not respond favorably to the root canal treatment. Apart from the discussion as to whether contamination can be effectively prevented during surgical sampling of apical periodontitis lesions, there are other issues that should be considered regarding studies of this nature.…”

Section: Where We Are Nowmentioning

confidence: 99%

Present status and future directions in endodontic microbiology

Siqueira¹,

Rôças²

2014

Endodontic Topics

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Apical periodontitis and its different manifestations are caused primarily by bacterial infection of the root canal system. Bacteria are usually organized in biofilm communities and can colonize not only the main canal but also spread to other areas in the root canal system. In these regions, bacteria are more difficult to reach and eliminate during treatment. The knowledge of endodontic infections has substantially increased over the past 4 decades, including the main species involved in the different forms of disease, their virulence factors, interactions, ecology, organization and spatial distribution in the root canal system, patterns of antimicrobial resistance, and so on. However, there is a need for all of this information to be translated into improvements in clinical practice and treatment outcomes. This article reviews the present status of endodontic microbiology, discusses perspectives for future research and directions, and emphasizes the need for a call to action in the field of applied endodontic microbiology.

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“…reported that Gram-positive microorganisms are more susceptible to lower concentrations of tetracycline (doxycycline in MTAD) than Gram-negative ones. [29] Fujii et al . stated the efficacy of doxycycline in endodontic application may be questionable as Gram-negative species dominate established infections.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial assay of combination surfactant irrigant regimen on vancomycin-resistant Enterococcus faecalis. An in vitro direct contact test

et al. 2018

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Background:Vancomycin-resistant enterococci (VRE) are on the rise globally in primary intraradicular infections and resistant to most intracanal irrigants and medicaments. The aim of this study was to evaluate the efficacy of irrigants and identify a cost-effective regimen to eradicate VRE.Materials and Methods:In this in vitro study irrigants were categorized as primary and surfactant groups with individual concentrations consisting of 10 samples each. Primary irrigants; sodium hypochlorite (NaOCl), chlorhexidine (CHX), and iodine potassium iodide (IKI) were prepared in concentrations of 5%, 2.5%, 2%, and 1%. Surfactants cetrimide (CTR) and sodium dodecyl sulfate (SDS) were prepared in concentrations of 0.25%, 0.5%, 1%, and 2%. Biopure MTAD was chosen as the control group. ATCC 51299 (VRE) was evaluated for antimicrobial susceptibility to the above irrigants by direct contact test for 5 min. The effect of each test irrigant was determined by calculating the percentage kill of viable bacteria by spectrophotometer. Statistical analysis was done by means of a one-way ANOVA and Mann–Whitney U-test (P < 0.05 consider significant).Results:About 2.5% and 5% CHX were significant over mixture of tetracycline, acid and detergent (MTAD) (P < 0.05). 5% CHX could achieve 100% elimination while 2.5% CHX and 5% IKI had 99.90%. 2% CHX and 2.5% IKI had 99% effective kill percentage. All concentrations of NaOCl were ineffective (90%) as compared to MTAD (95%). CTR (0.5%, 1% and 2%) and SDS (2%) were significant (P < 0.05) over MTAD. Combination surfactant regimens of 2% CHX +0.5% CTR and 2% CHX +1% SDS achieved 99.90% eradication potential and were significant (P < 0.05) over MTAD.Conclusion:Surfactant regimens were highly effective and superior to MTAD. CTR and SDS by their organic solvent property enhanced the antibacterial action of CHX.

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Bacterial diversity in persistent periapical lesions on root-filled teeth

Cited by 29 publications

References 51 publications

Microbial community in persistent apical periodontitis: a 16S rRNA gene clone library analysis

Microbial community in persistent apical periodontitis: a 16S rRNA gene clone library analysis

Present status and future directions in endodontic microbiology

Antimicrobial assay of combination surfactant irrigant regimen on vancomycin-resistant Enterococcus faecalis. An in vitro direct contact test

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