Bacterial immobilization and oxidation of arsenic in acid mine drainage (Carnoulès creek, France)

Casiot, Corinne; Morin, Guillaume; Juillot, Farid; Bruneel, Odile; Personné, Jean-Christian; Leblanc, Marc; Duquesne, Katia; Bonnefoy, Violaine; Elbaz-Poulichet, Françoise

doi:10.1016/s0043-1354(03)00080-0

Cited by 166 publications

(141 citation statements)

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“…(CARN2), associated with metabolisms such as iron oxidation and sulfur oxidation by Acidithiobacillus sp. (CARN5) and the Gallionella-related strain (CARN7), seems to be of prime importance in the co-precipitation of these inorganic elements, leading to the partial but efficient attenuation of this arsenic-contaminated ecosystem Casiot et al, 2003;Duquesne et al, 2003;Egal et al, 2009Egal et al, , 2010. Other microorganisms, in particular 'Candidatus Fodinabacter communificans,' may recycle organic compounds released by others or provide them with essential cofactors, improving their biomineralization activities.…”

Section: Discussionmentioning

confidence: 99%

“…This natural process of attenuation seems to be mainly due to microbial metabolism, leading to the oxidation of iron and arsenic into Fe(III) and As(V), and their co-precipitation with sulfur. In addition, laboratory experiments suggest that bacteria belonging to Thiomonas or Acidithiobacillus genera are involved in this process in situ Casiot et al, 2003;Duquesne et al, 2003;Morin et al, 2003;Egal et al, 2009). However, 16S-based community analyses have revealed that other genera are present in this ecosystem (Duquesne et al, 2003(Duquesne et al, , 2008Bruneel et al, 2003Bruneel et al, , 2006Bruneel et al, , 2008Bruneel et al, , 2011.…”

Section: Introductionmentioning

confidence: 99%

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Metabolic diversity among main microorganisms inside an arsenic-rich ecosystem revealed by meta- and proteo-genomics

et al. 2011

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By their metabolic activities, microorganisms have a crucial role in the biogeochemical cycles of elements. The complete understanding of these processes requires, however, the deciphering of both the structure and the function, including synecologic interactions, of microbial communities. Using a metagenomic approach, we demonstrated here that an acid mine drainage highly contaminated with arsenic is dominated by seven bacterial strains whose genomes were reconstructed. Five of them represent yet uncultivated bacteria and include two strains belonging to a novel bacterial phylum present in some similar ecosystems, and which was named 'Candidatus Fodinabacter communificans.' Metaproteomic data unravelled several microbial capabilities expressed in situ, such as iron, sulfur and arsenic oxidation that are key mechanisms in biomineralization, or organic nutrient, amino acid and vitamin metabolism involved in synthrophic associations. A statistical analysis of genomic and proteomic data and reverse transcriptase-PCR experiments allowed us to build an integrated model of the metabolic interactions that may be of prime importance in the natural attenuation of such anthropized ecosystems.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metabolic diversity among main microorganisms inside an arsenic-rich ecosystem revealed by meta- and proteo-genomics

et al. 2011

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“…Notons que l'adsorption par les hydroxydes de fer amorphes [am-Fe(OH) 3 ] est un facteur contrôlant le comportement de l'As(v) et As(iii) dans l'eau (SmEDLEy et KiNNiBURGH, 2002). La corrélation négative observée entre le Fe, mn et As (Figure 8) montre qu'un apport en Fe et mn réduit la concentration d'As dans les eaux (CASiOT et al, 2003;HALim et al, 2009). L'As présente une corrélation positive avec le Ca et, à moindre degré, avec le K. Cependant, des corrélations significatives avec les éléments étudiés dans les terrils de la mine de Jérissa et dans le sol avoisinant sont observées.…”

Section: Métaux Lourds Dans Les Eaux De La Nappeunclassified

Bassin d'oued Serrat : terrils et rejets domestiques, reconnaissance des métaux lourds et polluants, impact sur les eaux souterraines (nord-ouest de la Tunisie)

Mlayah

Silva

Hatira

et al. 2011

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Le présent travail visait à déterminer l'impact de rejets de deux mines de fer et de phosphates sur la qualité physicochimique de l'eau d'un système aquifère alluvial (nord-ouest de la Tunisie). Des échantillons de terrils, de sols ainsi que de l'eau de puits captant la nappe ont été prélevés et analysés. Les résultats d'analyses des échantillons de terrils ont montré que cette matrice renferme des concentrations remarquables en métaux lourds (essentiellement le Cd, Cr, Pb et Zn). Ces éléments métalliques ont été également détectés à des concentrations non négligeables dans la fraction fine des sols situés en aval des rejets miniers, suite à son transport éolien et hydrique. En ce qui concerne les eaux de la nappe, il s'avère que le transport des métaux issus des terrils est totalement atténué par les éléments constituant le sous-sol. Ces eaux, de bonne qualité géochimique, sont toutefois assez contaminées en nitrates dû aux pratiques agricoles intenses et à l'absence de systèmes d'assainissement urbains.The aims of this work were to determine the impacts of releases from two iron and phosphate mines (North West Tunisia) on the physico-chemical water composition of the related alluvial aquifer system. Samples from tailings, soil and water wells were collected and analyzed. The analytical results of tailing samples showed that this matrix contains high concentrations of heavy metals (mainly Cd, Cr, Pb and Zn). These metals were also detected at significant levels in the fine soil fraction downstream from the mine waste discharge due to transport by wind and water. The heavy metal concentrations in groundwater showed that the metal transport from the tailings is completely attenuated by the soil constituents. Overall, the physic-chemical quality of the groundwater is good, except for nitrates, which are present at relatively high concentrations due to intensive agricultural practices and the absence of urban wastewater treatment plants

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“…Subsequent follow-up characterizations of this organism and this process failed to materialize; however, approximately 2 decades later, Santini et al (52) described the isolation and initial characterization of a Rhizobiumlike bacterium (strain NT-26) that could grow chemolithoautotrophically with As III as a sole electron donor for energy generation and with CO 2 as a sole carbon source. Soon thereafter, and in part stimulated by the massive arsenic poisoning disaster in Bangladesh (2), a series of studies initiated the characterization of microbial As III oxidation in natural environments, including geothermal springs (9,11,12,17,19,24,25,35,51) and soils (41); in mining-contaminated environments (6,13,40); and, most recently, in anoxic photosynthesis (21,33 (28,31) indicated the role and importance of the sensor kinase AioS and its putative regulatory partner AioR (a bacterial enhancer binding protein), direct proof of these two proteins working together as part of a putative As III signal perception and transduction cascade was just recently provided by Sardiwal et al (54), who demonstrated the autophosphorylation of an AioS component and the AioS-specific phosphorylation of AioR. Recently, our work has expanded this regulatory model to now include a third component, AioX, which is a periplasmic As III binding protein that is also essential for aioBA expression (39 …”

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Involvement of RpoN in Regulating Bacterial Arsenite Oxidation

Kang

Bothner

Rensing

et al. 2012

Appl Environ Microbiol

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In this study with the model organism Agrobacterium tumefaciens, we used a combination of lacZ gene fusions, reverse transcriptase PCR (RT-PCR), and deletion and insertional inactivation mutations to show unambiguously that the alternative sigma factor RpoN participates in the regulation of As III oxidation. A deletion mutation that removed the RpoN binding site from the aioBA promoter and an aacC3 (gentamicin resistance) cassette insertional inactivation of the rpoN coding region eliminated aioBA expression and As III oxidation, although rpoN expression was not related to cell exposure to As III . Putative RpoN binding sites were identified throughout the genome and, as examples, included promoters for aioB, phoB1, pstS1, dctA, glnA, glnB, and flgB that were examined by using qualitative RT-PCR and lacZ reporter fusions to assess the relative contribution of RpoN to their transcription. The expressions of aioB and dctA in the wild-type strain were considerably enhanced in cells exposed to As III , and both genes were silent in the rpoN::aacC3 mutant regardless of As III . The expression level of glnA was not influenced by As III but was reduced (but not silent) in the rpoN::aacC3 mutant and further reduced in the mutant under N starvation conditions. The rpoN::aacC3 mutation had no obvious effect on the expression of glnB, pstS1, phoB1, or flgB. These experiments provide definitive evidence to document the requirement of RpoN for As III oxidation but also illustrate that the presence of a consensus RpoN binding site does not necessarily link the associated gene with regulation by As III or by this sigma factor. E vidence of microbial arsenite (As III ) oxidation was first reported nearly a century ago (18). Subsequent progress has been sporadic, with work that identified some organisms capable of As III oxidation (46,48,60) and then a study of a Pseudomonas arsenitoxidans strain reported to grow chemolithoautotrophically with As III as a sole electron donor (23). Subsequent follow-up characterizations of this organism and this process failed to materialize; however, approximately 2 decades later, Santini et al. (52) described the isolation and initial characterization of a Rhizobiumlike bacterium (strain NT-26) that could grow chemolithoautotrophically with As III as a sole electron donor for energy generation and with CO 2 as a sole carbon source. Soon thereafter, and in part stimulated by the massive arsenic poisoning disaster in Bangladesh (2), a series of studies initiated the characterization of microbial As III oxidation in natural environments, including geothermal springs (9,11,12,17,19,24,25,35,51) and soils (41); in mining-contaminated environments (6, 13, 40); and, most recently, in anoxic photosynthesis (21, 33). Likewise, progress has been made in the understanding of the biochemistry of the As III oxidase enzyme (1,14,37 oxidase structural genes were later cloned from the above-mentioned Rhizobium NT-26 organism (53). The symbols for genes coding for functions associated with As III oxidation have...

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Bacterial immobilization and oxidation of arsenic in acid mine drainage (Carnoulès creek, France)

Cited by 166 publications

References 25 publications

Metabolic diversity among main microorganisms inside an arsenic-rich ecosystem revealed by meta- and proteo-genomics

Metabolic diversity among main microorganisms inside an arsenic-rich ecosystem revealed by meta- and proteo-genomics

Bassin d'oued Serrat : terrils et rejets domestiques, reconnaissance des métaux lourds et polluants, impact sur les eaux souterraines (nord-ouest de la Tunisie)

Involvement of RpoN in Regulating Bacterial Arsenite Oxidation

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