Bacterial invasion and killing by predatory Bdellovibrio primed by predator prey cell recognition and self protection

Caulton, Simon G.; Lovering, A.L.

doi:10.1016/j.mib.2020.07.002

Cited by 13 publications

(5 citation statements)

References 47 publications

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“…Upon initial attachment, prey quality is assessed in a cyclic-di-GMP-dependent process (Caulton and Lovering, 2020; Hobley et al, 2012; Meek et al, 2019), and if the prey is found to be suitable and not already under attack by another predator (Lerner et al, 2012), the attachment is made permanent and the process of invasion begins. Initially, it was suggested that a “drilling” mechanism caused by flagellar rotation might play a role in the invasion process (Burnham et al, 1968; Stolp and Starr, 1965), but later studies found that flagellar motility-compromised mutants can still invade prey, which fits with the roles of pili and gliding motility mentioned above (Lambert et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Dynamic structural adaptations enable the endobiotic predation ofbdellovibrio bacteriovorus

Kaplan

Chang

Oikonomou

et al. 2022

Preprint

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Bdellovibrio bacteriovorusis an endobiotic microbial predator that offers promise as a living antibiotic for its ability to kill Gram-negative bacteria, including human pathogens. Even after six decades of study, fundamental details of its predation cycle remain mysterious. Here, we used cryo-electron tomography to comprehensively image the lifecycle ofB. bacteriovorusat nanometer-scale resolution. In addition to providing the first high-resolution images of predation in a native (hydrated, unstained) state, we also discover several surprising features of the process, including novel macromolecular complexes involved in prey attachment/invasion and a flexible portal structure lining a hole in the prey peptidoglycan that tightly seals the prey outer membrane around the predator during entry. Unexpectedly, we find thatB. bacteriovorusdoes not shed its flagellum during invasion, but rather resorbs it into its periplasm for degradation. Finally, following replication and division in the bdelloplast, we observe a transient and extensive ribosomal lattice on the condensedB. bacteriovorusnucleoid.Graphical abstract

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Section: Introductionmentioning

confidence: 99%

Dynamic structural adaptations enable the endobiotic predation ofbdellovibrio bacteriovorus

Kaplan

Chang

Oikonomou

et al. 2022

Preprint

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“… B. bacteriovorus consumes its Gram-negative bacterial prey in a process that typically lasts 3–4 h. This predatory lifecycle is a complex process that is beginning to be understood at the molecular level [26–33]. Initially, B.…”

Section: Predatory Lifestyle Of B Bacteriovorusmentioning

confidence: 99%

“…B. bacteriovorus consumes its Gram-negative bacterial prey in a process that typically lasts 3-4 h. This predatory lifecycle is a complex process that is beginning to be understood at the molecular level [26][27][28][29][30][31][32][33]. Initially, B. bacteriovorus recognizes, attaches to [34] and enters the prey cell, reinforcing, traversing and re-sealing its entry port [35].…”

Section: Predatory Lifestyle Of B Bacteriovorusmentioning

confidence: 99%

Predatory bacteria as living antibiotics – where are we now?

Atterbury

Tyson

2021

Microbiology

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Antimicrobial resistance (AMR) is a global health and economic crisis. With too few antibiotics in development to meet current and anticipated needs, there is a critical need for new therapies to treat Gram-negative infections. One potential approach is the use of living predatory bacteria, such as Bdellovibrio bacteriovorus (small Gram-negative bacteria that naturally invade and kill Gram-negative pathogens of humans, animals and plants). Moving toward the use of Bdellovibrio as a ‘living antibiotic’ demands the investigation and characterization of these bacterial predators in biologically relevant systems. We review the fundamental science supporting the feasibility of predatory bacteria as alternatives to antibiotics.

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“…, Staphylococcus aureus ) by entering the middle space between the wall and the out membrane of host bacteria to form bdelloplasts and multiplies 10 . Its unique action can be regarded as “parasitic” and “lytic” 11 . It is regarded as a replicable “live antibiotic”, coevolving with bacteria 12 .…”

Section: Introductionmentioning

confidence: 99%