Bacterial lactase genes diversity in intestinal mucosa of dysbacterial diarrhea mice treated with Qiweibaizhu powder

Abstract

The current research tried to investigate the effect of Qiweibaizhu powder (QWBZP) on intestinal mucosa bacterial lactase gene from dysbacterial diarrhea mice, as the good therapeutic mechanism of QWBZP on antibiotics-induced diarrhea. Dysbacterial diarrhea mice model was constructed by gastric perfusion with mixture of cephradine capsules and gentamicin sulfate (23.33 mL kg −1 day −1 ) for 5 days. After the success of establishing diarrhea, the mice in treatment group were gavaged with QWBZP for 3 days. Intestinal mucosa in each group was collected, and diversity of bacterial lactase genes in intestinal mucosa of mice was carried out by Miseq metagenome sequencing. The results showed the Chao1, ACE, Simpson and Shannon indices in treatment group were lower than model group and were similar to control group. The same result was obtained from the operational taxonomic units (OTUs). There were 298, 435 and 254 OTUs in the control group, model group and treatment group, respectively. Principal component analysis (PCA) indicated that samples distribution in both normal and treatment groups were relatively intensive, distances among individuals were small, while opposite results were observed in model group. Moreover, antibiotics increased the diversity and abundance of bacterial lactase genes at phylum and genus levels. However, they decreased and were similar to control group after treating with QWBZP. Our results indicate that QWBZP has a positive effect on the recovery of bacterial lactase gene diversity to normal level. In addition, QWBZP increase the abundance of Lysobacter and Eukaryota.

Bacterial Diversity in the Intestinal Mucosa of Dysbiosis Diarrhea Mice Treated with Qiweibaizhu Powder

“…It was boiled twice, and the decoction time was usually 20-30 min. The liquid medicine decocted twice was mixed to make a 100% dose of a traditional decoction of QWBZP and frozen at 4°C for the following experiment [15,16].…”

“…Cephradine capsules (product batch number: 110804) and gentamicin sulfate injection (product batch number: 5120106) were purchased from Suzhou Chung-Hwa Chemical & Pharmaceutical Industrial Co., Ltd. and Yichang Renfu Pharmaceutical Co., Ltd., respectively. An antibiotic mixture was prepared at a concentration of 62.5 g L -1 with sterile saline according to the ratio of 1 : 2 (i.e., 6 gentamicin (2 mL/branch) + 3 cephalosporins (0.25 g/grain)) and stored at 4°C [16,17]. Protease K, Tris-saturated phenol-chloroform-isoamyl alcohol (25 : 24 : 1), lysozyme, and TE buffer were purchased from Beijing Ding Guo Biotechnology Co., Ltd. Other reagents were prepared in the lab.…”

“…To induce diarrhea, mice in both the model group and treatment group were administered an antibiotic mixture composed of gentamicin sulfate and cephradine (23.33 mL•kg -1 •d -1 ). Correspondingly, mice in the normal group were gavaged with sterile water, 0.35 mL twice a day for 5 days [11,16]. When diarrhea symptoms (declined activity, arched back trembling, specifically watery stool, curled up, and poor appetite) were induced, the mice in the treatment group were administered the QWBZP decoction at a dosage of 0.16 g•kg -1 •d -1 for 3 days [12,16].…”

“…Correspondingly, mice in the normal group were gavaged with sterile water, 0.35 mL twice a day for 5 days [11,16]. When diarrhea symptoms (declined activity, arched back trembling, specifically watery stool, curled up, and poor appetite) were induced, the mice in the treatment group were administered the QWBZP decoction at a dosage of 0.16 g•kg -1 •d -1 for 3 days [12,16]. Mice were sacrificed using cervical vertebra dislocation on a sterile operation platform.…”

Role of Tryptophan-Metabolizing Microbiota On Diarrhea Mice Caused by Folium sennae Extracts

“…Referring to our previous experiment [39], 500 g Folium sennae was washed in cold water and naturally dried. The cleaned Folium sennae was soaked in 5 L boiling water for 10 minutes.…”