Bacterial Pathogens and Antibiotic Susceptibility Patterns of Cervico-Vaginal Discharges in Cross Bred Repeat Breeding Heifer Cows

Ahuja, Ankit Kumar; Cheema, Ranjna S.; Narang, Deepti; Dhindsa, S. S.

doi:10.20546/ijcmas.2017.606.205

Cited by 4 publications

(5 citation statements)

References 9 publications

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“…Similar results were also obtained by El-Jakee et al, (2008), who reported bacterial culture from 100 per cent of vaginal samples during normal estrous cycle. However, these findings are in contrary to the previous reports of 53.9 (Ocando et al, 2010;Zambrano-Nava et al, 2011) and 54.5 (Ahuja et al, 2017) per cent cultural positive samples from normal fertile cows.…”

Section: Bacterial Isolates From Vagina Of Cyclic Cowscontrasting

confidence: 97%

Isolation of Bacteria from the Vaginal Aspirates of Cyclic, Acyclic, Endometritic and Pregnant Crossbred Cows

Patel¹,

Panchal²,

Dhami³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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Section: Bacterial Isolates From Vagina Of Cyclic Cowscontrasting

confidence: 97%

Isolation of Bacteria from the Vaginal Aspirates of Cyclic, Acyclic, Endometritic and Pregnant Crossbred Cows

Patel¹,

Panchal²,

Dhami³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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“…and Staphylococcus sp. cultured in our previous study (Ahuja, Cheema, Narang, & Dhindsa, ) were suspended in 1.0 ml of 2% SDS in 62.5 mMTris‐HCl (pH 6.8) containing protease inhibitors (Cocktail, SERVA, New Delhi) in different tubes. Sperm and bacterial suspension were sonicated at 20 Watts for 3 × 20 s, and then centrifuged at 9615 g for 15 min (Cheema, Bhakri, & Vashishat, ).…”

Section: Methodsmentioning

confidence: 99%

“…and Staphylococcus sp. cultured in our previous study (Ahuja, Cheema, Narang, & Dhindsa, 2017) were suspended in 1.0 ml of 2% SDS in 62.5 mMTris-HCl (pH 6.8) containing protease inhibitors (Cocktail, SERVA, New Delhi) in different tubes.…”

Section: Extraction Of Sperm and Bacterial Proteinsmentioning

confidence: 99%

Homology between cattle bull sperm and bacterial antigenic proteins viz a viz possible role in immunological infertility

Ahuja

Cheema

2018

Reprod Domestic Animals

Self Cite

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This study was carried out to investigate the possible presence of identical sperm and bacterial antigens which may cause similar antisperm antibody production leading to lower fertility. Cross-reactive antigens of cattle bull spermatozoa and different bacteria including Escherichia coli (E. coli), Bacillus sp., and Staphylococcus sp. were characterized by immunoblotting and mass fingerprinting. Significant cross-reactivity was obtained for 75, 72, 44, 40, 33, 30, 25, 18, 14 kDa proteins with purified IgG of calves, heifers and cows between spermatozoa and the studied bacteria. Significantly (p < 0.05) matched cross-reactive 40/33/30 kDa sperm, 33 kDa Staphylococcus sp/Bacillus sp and 40/25 kDa E. coli proteins were analyzed. Mass fingerprinting of 40/33/30 kDa (spermatozoa); 40/25 kDa (E. coli) and 33 kDa (Bacillus/Staphylococcus) proteins revealed their matching with vitellogenin-1-like/mitochondrial malate dehydrogenase 2, NAD/acrosin-binding protein isoform XI; outer membrane insertion signal domain/spore coat protein and glyceraldehyde-3-phosphate dehydrogenase, respectively. Acrosin-binding protein isoform X1 and mitochondrial malate dehydrogenase 2, NAD contributes to the capacitation of spermatozoa. Spore coat protein; glyceraldehyde-3-phosphate dehydrogenase of E. coli; Bacillus/Staphylococcus are 37.6% and 39.01% identical to acrosin-binding protein isoform X1; mitochondrial malate dehydrogenase 2, NAD of cattle bull spermatozoa. It can be interpreted from these observations that cross-reacting antibodies developed against 33/30 kDa sperm proteins and 25, 33 kDa bacterial proteins in cows may affect the functional activity of spermatozoa leading to delayed fertility in heifers and cows.

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“…Among different lactating Holstein dairy farms, four papers of the referred works 44,[92][93][94] applied honey apitheray were dealt with three dairy farm problems:…”

Section: Honey Apitherapy In Lactating Dairy Cowsmentioning

confidence: 99%

Testimony for veterinary apitherapy

MM¹

2019

IJCAM

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Background Nowadays by the indiscriminate use of antibiotics, multi-drug resistant pathogens (MDR) developed and spread, so searching for effective natural antimicrobials is of global concern. Apitherapy possessing various therapeutic activities (antimicrobial, 1,2 anti-inflammatory, 3-5 antioxidant, 6-9 antiproliferative, 10 immunomodulator 11 ameloirative 12,13 or wound healing factor) 14,15 proved to be a suitable tool to get the target since it is very safe, highly effective, easily applicable and extremely economic. The subject is of interesting concept for the apitherapy research team affiliating to

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Bacterial Pathogens and Antibiotic Susceptibility Patterns of Cervico-Vaginal Discharges in Cross Bred Repeat Breeding Heifer Cows

Cited by 4 publications

References 9 publications

Isolation of Bacteria from the Vaginal Aspirates of Cyclic, Acyclic, Endometritic and Pregnant Crossbred Cows

Isolation of Bacteria from the Vaginal Aspirates of Cyclic, Acyclic, Endometritic and Pregnant Crossbred Cows

Homology between cattle bull sperm and bacterial antigenic proteins viz a viz possible role in immunological infertility

Testimony for veterinary apitherapy

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