Isolation of Bacteria from the Vaginal Aspirates of Cyclic, Acyclic, Endometritic and Pregnant Crossbred Cows

Patel, Chintan; Panchal, M. T.; Dhami, Amarjeet; Bhanderi, B. B.; Mathakiya, R.A.

doi:10.20546/ijcmas.2019.803.065

Cited by 4 publications

(11 citation statements)

References 11 publications

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“…The dominance of estrogen during the follicular phase of the estrous cycle increases the rate of migration of leucocyte into the uterine and vaginal lumen, and thus increases the bactericidal activity. The results of the present study corroborated with the findings of El-Jakee et al 2008, Kavyashree (2013) and Patel et al (2019), who also reported that the bacteria isolated during luteal phase were found to be higher than those found in follicular phase of estrous cycle. However, Vlcek and Svobodova (1985) reported contrary findings of 40.90 % samples with bacterial isolates during the follicular phase and 35.36 % during luteal phase of the normal estrous cycle in cows.…”

Section: Bacterial Isolates From Vaginal Mucus/aspirates Of Cyclic Busupporting

confidence: 92%

“…were the common isolates from the endometritic buffaloes in the present study. These findings are in consonance with the observations reported earlier by Azawi et al (2008), Atchawaran et al (2013), Barman et al (2013) and Patel et al (2019). However, in contrary to the present findings, Patel et al (2009) reported only 55.55% samples showing bacterial growth in postpartum endometritic buffaloes.…”

Section: Bacterial Isolates From Vagina Of Acyclic and Endometritic Bsupporting

confidence: 91%

“…and Staphylococcus spp. from the vaginal swabs of the anestrus buffalo-cows, and almost similar were the findings of Patel et al (2019) in crossbred cows. Clinically acyclic buffaloes have the state of ovarian inactivity with an absence of circulatory estrogen.…”

Section: Bacterial Isolates From Vagina Of Acyclic and Endometritic Bsupporting

confidence: 77%

“…There are scattered reports of the routine cultural isolation of bacteria from the genital discharges of infertile bovine females. Still, not much is known about normal vaginal microflora of cyclic, acyclic, pregnant, and endometritic cattle or buffalo (Patel et al, 2019). Hence, buffaloes of University farm, AAU, Anand from August 2018 to June 2019.…”

Section: Bacterial Isolates From the Genital Aspirates Of Cyclic Acymentioning

confidence: 99%

“…The samples of cervicovaginal mucus/discharge during estrus/endometritis, and vaginal washings/aspirates during other phases of the estrous cycle, i.e., proestrus, metestrus, diestrus, and anestrus as well as pregnancy were collected aseptically using syringe and pipette method. For the collection of vaginal washing, the pointed end of glass pipette was connected to a 60 mL syringe with rubber junction and 30 mL sterile normal saline was first infused in the vaginal fornix, massaged per rectally for few minutes and again aspirated with the same pipette aseptically (Patel et al, 2019). Isolation of bacteria was carried out as per Cruickshank (1965) by inoculation of samples on blood agar and MacConkey agar plates.…”

Section: Bacterial Isolates From the Genital Aspirates Of Cyclic Acymentioning

confidence: 99%

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Bacterial Isolates from the Genital Aspirates of Cyclic, Acyclic, Endometritic and Pregnant Buffaloes

et al. 2019

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The study was carried out on 50 vaginal secretions/aspirates/discharge samples collected aseptically using syringe and pipette method from infertile (anestrus; endometritic, n = 6 each) buffaloes of villages nearby Anand and healthy cyclic (n = 5; proestus, estrus, metestrus, diestrus) as well as 3, 6 and 9 month pregnant (n=6 each) buffaloes of University farm to identify the vaginal microorganisms based on routine cultural examination. In all 117 bacterial isolates were recovered from all 50 vaginal samples (100 %) of 35 buffaloes during different physio-pathological status. The bacteria isolated from vaginal mucus/aspirates of buffaloes during the follicular phase comprised Corynebacterium spp. as the most predominant isolate (28.57%) followed by E. coli, Bacillus Spp., Staphylococcus spp., Streptococcus spp., Salmonella spp., Proteus spp., and vaginal yeast, whereas during the luteal phase, the most predominant bacteria were E. coli (23.33%) followed by Corynebacterium spp., Bacillus spp., Staphylococcus spp., Streptococcus spp., and Klebsiella spp. In acyclic buffaloes, the most predominant bacteria isolated were Corynebacterium spp. (21.43%) Bacillus spp., Micrococcus spp., Pseudomonas, Staphylococcus spp., Streptococcus spp., E. coli and Salmonella spp., whereas the endometritic buffaloes evinced the most predominant bacterial isolates as Corynebacterium spp. and E. coli (20.00% each) followed by Bacillus spp., Salmonella, Proteus spp., Staphylococcus spp., Streptococcus spp., and Klebsiella spp. The major bacteria isolated during the entire period of pregnancy were E. coli, Micrococcus, Corynbacterium Spp., Bacillus spp., Staphylococcus spp. and Proteus. This study concludes rich bacterial diversity in the vagina of buffaloes during different physio-pathological status.

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Section: Bacterial Isolates From Vaginal Mucus/aspirates Of Cyclic Busupporting

confidence: 92%

Section: Bacterial Isolates From Vagina Of Acyclic and Endometritic Bsupporting

confidence: 91%

Section: Bacterial Isolates From Vagina Of Acyclic and Endometritic Bsupporting

confidence: 77%

Section: Bacterial Isolates From the Genital Aspirates Of Cyclic Acymentioning

confidence: 99%

Section: Bacterial Isolates From the Genital Aspirates Of Cyclic Acymentioning

confidence: 99%

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Bacterial Isolates from the Genital Aspirates of Cyclic, Acyclic, Endometritic and Pregnant Buffaloes

et al. 2019

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Profil bakteri non spesifik dalam lendir serviks sapi perah pada fase folikuler dan fase luteal

Nur¹,

Mulyati²,

Sardjito³

et al. 2020

Ovz

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This research was conducted to identify non-specific bacteria found in the cervical mucus of dairy cattle in the follicular and luteal phase. Twenty cervical mucus samples were collected from ten cows during their follicular phase and ten cows during their luteal phase. Sampling was carried out with the help of an artificial insemination gun and a plastic sheath, which were protected by a sheath cover. After insertion into cervix, the tip of the plastic sheath was cut and put into a tube prefilled with phosphate buffered saline. All samples were cultured in Blood Agar media and isolated in Eosin Methylene Blue Agar, Mac Conkey Agar, and Manitol Salt Agar. Gram staining was applied to distinguish gram-positive and gram-negative bacteria; and followed with the catalase, spore, motility, and biochemical tests to further identify bacteria. The bacteria identified were of the genus Enterobacter, Staphylococcus, Corynebacterium, Streptococcus, Escherichia, and Pasteurella. It could be concluded that non-specific bacteria of the same genus were identified in cervical mucus of dairy cows in their follicular and luteal phase, in a various of percentages.

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The Number and Types of Bacteria in the Bali Cattle’s Vagina during Estrus and Pregnant

Tono¹,

Sudipa²,

Suarjana³

2020

J.Veterinary and Animal Sciences

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Bali cattle have very good potential for meat and calf supply, because they have good adaptability with good quality reproductive properties. However, good reproductive nature is threatened by diseases caused by bacteria. This study aims to determine the number and types of bacteria in the vagina of Bali cattle that are estrus and pregnant. Sample using vaginal swabs from 30 cows. The sample consisted of 10 swabs from non-estrus and not pregnant (control / normal) cattle, 10 swabs from estrus cows, and 10 swabs from pregnant cows. After the swab is carried out using a cotton bud, the results of the swab are stored in Stuart transport media. Samples planted in blood media to be identified and the number of colonies counted, then followed by Gram staining and biochemical tests then the results were presented descriptively. The result is the highest number of estrous cow colonies (1034 colonies), compared to non-estrous and non-pregnant cows (407 colonies) and pregnant (376 colonies). This type of bacteria is dominated by Klebsiella sp. (42.85%), Eschericia coli (28.7%), Streptococcus sp. (14.28%) and Bacillus sp. (14.28%).

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Isolation of Bacteria from the Vaginal Aspirates of Cyclic, Acyclic, Endometritic and Pregnant Crossbred Cows

Cited by 4 publications

References 11 publications

Bacterial Isolates from the Genital Aspirates of Cyclic, Acyclic, Endometritic and Pregnant Buffaloes

Bacterial Isolates from the Genital Aspirates of Cyclic, Acyclic, Endometritic and Pregnant Buffaloes

Profil bakteri non spesifik dalam lendir serviks sapi perah pada fase folikuler dan fase luteal

The Number and Types of Bacteria in the Bali Cattle’s Vagina during Estrus and Pregnant

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