Bacterial Translocation and Endotoxemia After Pringle Maneuver in Cirrhotic Rats

Su, Yonghui; Pan, Haiyan; Guo, Zhaowang; Zhou, Wenying; Zhang, Baimeng

doi:10.1007/s10620-014-3381-2

Cited by 6 publications

(3 citation statements)

References 15 publications

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“…PM, which is commonly used for intraoperative bleeding control in liver surgeries, has been shown to lead BT in mesenteric lymph nodes and portal blood specimens in various experimental and clinical studies [18–21]. Our study also showed that PM causes bacterial growth in portal blood and mesenteric tissue cultures in rats.…”

Section: Discussionsupporting

confidence: 68%

The effect of immunonutrition on bacterial translocation after Pringle maneuverer in rats

Subaşı

Yıldız

Yüksel

et al. 2019

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IntroductionTemporary clamping of the hepatoduodenal ligament (the Pringle manoeuvre) is the most commonly used method to prevent intraoperative blood loss in liver surgery. Some side effects of the Pringle manoeuvre (PM) can occur.AimTo investigate the effectiveness of preoperative immunonutritional support to prevent bacterial translocation (BT) in rats due to PM.Material and methodsForty Wistar albino rats were randomly divided into four groups. Groups 1 and 2 were fed with normal rat diet and water, and groups 3 and 4 were fed with enteral immunonutrition (Impact Glutamine) containing 1 g/kg/day amino acid in the preoperative period. Group 1 (n = 10) and 4 (n = 10) rats were treated only with laparotomy; group 2 (n = 10) and 3 (n = 10) rats were treated with PM for 30 min with laparotomy. After 30 min, relaparotomy was applied to all groups and portal blood, mesentery, spleen samples were taken for culture purposes.ResultsProliferation in portal blood cultures was significantly higher in the samples from the normally fed group (group 2) in whom PM was applied, compared to the other groups (p < 0.001). No proliferation was observed in the PM-treated group (group 3), who also received preoperative immunonutritional support.ConclusionsPreoperative immunonutritional support is effective in the prevention of BT due to PM in rats.

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Section: Discussionsupporting

confidence: 68%

The effect of immunonutrition on bacterial translocation after Pringle maneuverer in rats

Subaşı

Yıldız

Yüksel

et al. 2019

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“…A midline laparotomy was performed in 6-IG, 12-IG, 24-IG groups. The PT was ligated according to the standard technique (19) for 6, 12 and 24 minutes, depending on the group of laboratory animals. Successfully performed PT clamping was determined and assessed by the detection of congestion in the intra-abdominal organs.…”

Section: Surgical Proceduresmentioning

confidence: 99%

“…Preclinical studies indicated a high risk of damage to the small intestine during clamping of the PT. It has been associated with hypo-perfusion of extra-hepatic structures that lead to the manifestation of bacterial translocation and endo-toxinemia (17)(18)(19). However, the different modes of PT clamping cannot provide a clear picture for establishing the optimal time interval to prevent irreversible changes in the structure of the small intestine.…”

Section: Introductionmentioning

confidence: 99%

The Impact of Cross-Clamping of The Portal Triad on The Morphology of The Small Intestine

Almabayev

Salimgereeva

Kamyspayev

et al. 2022

Experimental and Applied Biomedical Research (EABR)

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At present, clamping of the portal triad is a widespread surgical procedure in hospitals. Such an operation can prevent pathological changes in the organs. However, the optimal time for clamping remains unclear. To determine the starting time of irreversible morphological changes in the small intestine due to the clamping of the portal triad. The study was carried out on rats (n=94). Animals were randomly subdivided into 4 groups based on the duration of clamping of the portal triad (PT): I control group (CG; without clamping the PT; n=10); II intervention group (6-IG; clamping PT for 6 min; n=28); III intervention group (12-IG; clamping time of the PT for 12 min; n=28); IV intervention group (24-IG; clamping time of the PT for 24 min; n=28). In groups 6- IG, 12-IG, 24-IG, after clamping the portal triad, animals were withdrawn from the experiment after 3 hours, 6 hours, 12 hours, 1 day, 3 days and 7 days. Morphological changes in the small intestine were assessed by measuring the diameter of the lumen of micro-vessels. In addition, the mortality in the groups was analysed as well. In the CG group, the diameter of the arterioles of the small intestine was 34±4 μm, the diameters of pre-capillaries were 15±2μm, the capillaries were 5.4±1 μm, the post-capillaries were 18±2 μm, and the diameter of the lumen of the venues was 40±3 μm. In the 6-IG group (on the 3rd day), the structure of the small intestine showed the recovery signs. By the 7th day, the indicators returned to their original values. In the 12-IG group, the parameters of the small intestine were restored on the seventh day that corresponds to the usual course of the disease. However, in the 24-IG group, changes in these organs persisted until the end of the study. No deaths were reported in the CG and 6-IG animal groups. Mortality among rats of the 12-IG group was 14.3%, while in the 24-IG group with PT clamping for 24 minutes it was 42.8%, respectively. The morphological changes in the microvasculature of the small intestine after 6-minute PT clamping showed a tendency to recover (back to the control parameters). Nevertheless, after 24 minutes of clamping, the changes in the intestinal tissue were irreversible.

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Gut microbiota and bacterial translocation in digestive surgery: the impact of probiotics

Komatsu

Yokoyama

Nagino

2017

Langenbecks Arch Surg

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Bacterial Translocation and Endotoxemia After Pringle Maneuver in Cirrhotic Rats

Abstract: There is endotoxemia immediately after Pringle maneuver and gut bacteria translocation 24 h later. The incidence of gut bacterial translocation increases with the duration extension of Pm. Selective intestinal decontamination can decrease bacteria translocation and endotoxemia.

Cited by 6 publications

References 15 publications

The effect of immunonutrition on bacterial translocation after Pringle maneuverer in rats

The effect of immunonutrition on bacterial translocation after Pringle maneuverer in rats

The Impact of Cross-Clamping of The Portal Triad on The Morphology of The Small Intestine

Gut microbiota and bacterial translocation in digestive surgery: the impact of probiotics

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