Bacterial vaginosis and inflammatory response showed association with severity of cervical neoplasia in HPV‐positive women

Abstract

Vaginal infections may affect susceptibility to and clearance of human papillomavirus (HPV) infection and chronic inflammation has been linked to carcinogenesis. This study aimed to evaluate the association between bacterial vaginosis (BV) and inflammatory response (IR) with the severity of cervical neoplasia in HPV-infected women. HPV DNA was amplified using PGMY09/11 primers and genotyping was performed using a reverse line blot hybridization assay in 211 cervical samples from women submitted to excision of the transformation zone. The bacterial flora was assessed in Papanicolaou stained smears, and positivity for BV was defined as 20% of clue cells. Present inflammatory response was defined as 30 neutrophils per field at 10003 magnification. Age higher than 29 years (OR:1.91 95% CI 1.06-3.45), infections by the types 16 and/or 18 (OR:1.92 95% CI 1.06-3.47), single or multiple infections associated with types 16 and/or 18 (OR: 1.92 CI 95% 1.06-3.47), BV (OR: 3.54 95% CI 1.62-7.73) and IR (OR: 6.33 95% CI 3.06-13.07) were associated with severity of cervical neoplasia (CIN 2 or worse diagnoses), while not smoking showed a protective effect (OR: 0.51 95% CI 0.26-0.98). After controlling for confounding factors, BV(OR: 3.90 95% CI 1.64-9.29) and IR (OR: 6.43 95% CI 2.92-14.15) maintained their association with the severity of cervical neoplasia. Bacterial vaginosis and inflammatory response were independently associated with severity of cervical neoplasia in HPV-positive women, which seems to suggest that the microenvironment would relate to the natural history of cervical neoplasia. Diagn. Cytopathol. 2016;44:80-86. V C 2015 Wiley Periodicals, Inc.

Profile of women with cervical cancer attended for treatment in oncology center

“…Confronting these results, Castro-Sobrinho et al, 28 evaluating cervical smears of HPV-infected women, found that Gardnerella vaginalis was the most frequent microorganism in smears with invasive carcinoma (18.2%) and HSIL (45.6%), concluding that bacterial vaginosis, may be associated with the severity of cervical neoplasia.…”

The Effect of Magnesium and Vitamin E Co-Supplementation on Glycemic Control and Markers of Cardio-Metabolic Risk in Women with Polycystic Ovary Syndrome: A Randomized, Double-Blind, Placebo-Controlled Trial

“…A considerable decrease in triglycerides concentrations was also seen following the supplementation of 600 mg/day magnesium as magnesium oxide for 8 weeks among people with T2DM [26]. Moreover, vitamin E and omega-3 fatty acids co-supplementation for 12 weeks to women with PCOS significantly improved lipid profiles [27]. In an animal study, vitamin E supplementation for 20 weeks reduced serum levels of triglycerides and VLDL-cholesterol, but did not affect other lipid profiles [28].…”

CircRNA PLOD2 enhances ovarian cancer propagation by controlling miR-378

“…Lysyl hydroxylase (LH) is a protein encoded by procollage-lysine 2-oxoglutarate 5-dioxygenase (PLOD), which is a key enzyme mediating collagen-mediated oxygenase. PLOD gene families can be divided into three subtypes PLOD1, PLOD2, PLOD3, each subtype of the corresponding lysine hydroxylase gene encoding, the family belongs to 2-keto glutaric acid dependent two-plus oxygen members of the family of enzymes, with qi acid hydroxylase activity ( Shao et al, 2018 ), can be in Fe 2+ and 2 - keto glutaric acid is formed under the condition of homologous dimers, catalytic single former collagen lysine hydroxyl, lysine hydroxylation of collagen can be cross linked after secrete extracellular form stable structure of hydroxyl lysine pyridine chain ( Maria de Castro-Sobrinho et al, 2017 ). When the expression of PLOD2 is significantly increased in cells, the generation of hydroxylysine pyridine chain is increased, which will lead to excessive deposition of collagen fiber, damage of ECM structure, and further cause tumor progression and metastasis.…”