Bactericidal effects and accelerated wound healing using Tb4O7 nanoparticles with intrinsic oxidase-like activity

Abstract

Background Nanomaterials that exhibit intrinsic enzyme-like characteristics have shown great promise as potential antibacterial agents. However, many of them exhibit inefficient antibacterial activity and biosafety problems that limit their usefulness. The development of new nanomaterials with good biocompatibility and rapid bactericidal effects is therefore highly desirable. Here, we show a new type of terbium oxide nanoparticles (Tb 4 O 7 NPs) with intrinsic oxidase-like activity for in vitro and in vivo antibacterial application. Results We find that Tb 4 O 7 NPs can quickly oxidize a series of organic substrates in the absence of hydrogen peroxide. The oxidase-like capacity of Tb 4 O 7 NPs allows these NPs to consume antioxidant biomolecules and generate reactive oxygen species to disable bacteria in vitro. Moreover, the in vivo experiments showed that Tb 4 O 7 NPs are efficacious in wound-healing and are protective of normal tissues. Conclusions Our results reveal that Tb 4 O 7 NPs have intrinsic oxidase-like activity and show effective antibacterial ability both in vitro and in vivo. These findings demonstrate that Tb 4 O 7 NPs are effective antibacterial agents and may have a potential application in wound healing. Electronic supplementary material The online version of this article (10.1186/s12951-019-0487-x) contains supplementary material, which is available to authorized users.

Nanomaterials in wound healing: From material sciences to wound healing applications

“…Tb4O7 nanoparticles with intrinsic oxidase-like activity Oxidase-like activity; Antibacterial properties. [65] Titanium dioxide nanoparticles Titatnium dioxide nanoparticles/gelatin composite Antibacterial activity; High percentage of wound contraction; Fibroblasts growth stimulation; Epithelialization stimulation. [93] Titanium dioxide nanoparticle loaded chitosan-pectin nano dressing Biocompatibility; Inherent bioactivity of chitosan; Mechanical and antibacterial properties of TiO2.…”

Recent Progress of Nanozymes in the Detection of Pathogenic Microorganisms

“…and E. coli. [92] Many other metal oxides, such as CeO 2-x , [93][94][95] Tb 4 O 7 , [96] [97,98] have also been demonstrated high enzyme mimetic activities. They also can be used for pathogen detection.…”

Development of Novel Microenvironments for Promoting Enhanced Wound Healing

“…As previously discussed, growth factors and bactericidal chemicals are valuable components for drug delivery to wound sites, which may have a significant impact on wound closure rates, cell viability, and cell proliferation. Some labs have developed nanoparticles for delivery of these components, including bactericidal terbium oxide (Tb 4 O 7 ) nanoparticles and antimicrobial and growth factor-delivering PLGA nanoparticles [120,121]. The Tb 4 O 7 NPs were found to have intrinsic oxidase-like activity at acidic pH levels and behave as enzymes (nanozymes), while the PLGA NPs encapsulated VEGF, basic fibroblast growth factor (bFGF), and antimicrobial peptide (AMP) K4, and were designed to protect their cargo from enzymatic degradation.…”