Bacteriology of moderate (chronic) periodontitis in mature adult humans

Abstract

A total of 171 taxa was represented among 1,900 bacterial isolates from 60 samples of sites affected with moderate periodontitis in 22 mature adult humans. The composition of the subgingival sulcus flora was statistically significantly different from that of the adjacent supragingival flora and the subgingival flora of 14 people with healthy gingiva, but was not significantly different from that of sulci affected with severe periodontitis in 21 young human adults. The sulcus floras of moderate periodontitis and severe periodontitis shared many of their predominant bacterial species, but there were differences in the relative proportions of some of these species. Similar relationships were found for seven taxa of treponemes that were cultured from the samples.

Blastogenesis and polyclonal immunoglobulin synthesis in murine spleen cells stimulated with lipoolysaccharide, lipid A and acid-degrade polysaccharide from Fusobacterium nucleatum

“…For this reason, and also because of known osteoclastic activity [7], and the capacity to activate macrophages [8] and to stimulate polymorphonuclear leukocyte migration in vivo [6,15], F. nucleatum LPS may be considered a pathogenetic factor in periodontal disease. The organism is present in relatively large numbers in the subgingivai mieroflora of young adults with severe periodontitis [19] and in adults with chronic periodontitis [20].…”

The occurrence of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Bacteroides intermedius in destructive periodontal disease in adults

“…B. gingivalis has been strongly implicated in rapidly progressing periodontitis in adults (Slots 1982a, 1984, Tanner et al 1979). An alternative view by Moore et al (1983) suggests that B. gingivalis at least is not causing early periodontal destruction but arises merely as a result of the disease. B. intermedius has been recovered in large numbers from deep adult periodontitis lesions (Slots 1982a, Dzink et al 1983).…”

Detection of Slackia exigua, Mogibacterium timidum and Eubacterium saphenum from pulpal and periradicular samples using the Polymerase Chain Reaction (PCR) method

“…Asaccharolytic and Gram-positive rods use peptides for their growth, and may be associated with proteolytic activities in sites of inflammation that are diseased. In fact, organisms of oral AAGPR, including S. exigua, M. timidum and E. saphenum are isolated from various oral sites, such as infected dental pulps (Hoshino et al 1992a, Sato et al 1993, infected periapical lesions (Kiryu et al 1994), carious dentine (Edwardsson 1974, Hoshino 1985, Ando & Hoshino 1990), periodontal pockets with advanced periodontitis (Holdeman et al 1980, Moore et al 1983, Martin et al 1986, Hill et al 1987, Uematsu & Hoshino 1992, Wade et al 1992) and acute dento-alveolar abscesses (Wade et al 1994), when strict anaerobic procedures are adopted. S. exigua in particular has been found frequently in periradicular lesions (Sato et al 1993), and M. timidum and E. saphenum in periodontal pockets (Uematsu & Hoshino 1992, Uematsu et al 1996, although the latter two bacterial species were detected in periradicular lesions in the present study.…”

“…1994, in addition to other oral sites (Edwardsson 1974, Holdeman et al . 1980, Moore et al . 1983, Hoshino 1985, Martin et al .…”