Baculovirus ODV-E66 degrades larval peritrophic membrane to facilitate baculovirus oral infection

Hou, Dianhai; Kuang, Wenhua; Luo, Sijiani; Zhang, Fenghua; Zhou, F.; Chen, Tong; Zhang, Yanfang; Wang, Hualin; Hu, Zhihong; Deng, Fei; Wang, Manli

doi:10.1016/j.virol.2019.08.027

Cited by 16 publications

(12 citation statements)

References 52 publications

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“…Interestingly, only a single copy of this gene was found in CmNV (ORF56; Figure 3 B). The baculovirus ODV-E66 protein plays an important role in oral infectivity and may help the virus to overcome the peritrophic membrane lining the midgut [ 50 ]. Whether increased levels of ODV-E66 would give these two nudiviruses a particular benefit in their crustacean hosts or their environment awaits further analysis.…”

Section: Resultsmentioning

confidence: 99%

Identification and Full Characterisation of Two Novel Crustacean Infecting Members of the Family Nudiviridae Provides Support for Two Subfamilies

Bateman

Kerr

Stentiford

et al. 2021

Viruses

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Multiple enveloped viruses with rod-shaped nucleocapsids have been described, infecting the epithelial cell nuclei within the hepatopancreas tubules of crustaceans. These bacilliform viruses share the ultrastructural characteristics of nudiviruses, a specific clade of viruses infecting arthropods. Using histology, electron microscopy and high throughput sequencing, we characterise two further bacilliform viruses from aquatic hosts, the brown shrimp (Crangon crangon) and the European shore crab (Carcinus maenas). We assembled the full double stranded, circular DNA genome sequences of these viruses (~113 and 132 kbp, respectively). Comparative genomics and phylogenetic analyses confirm that both belong within the family Nudiviridae but in separate clades representing nudiviruses found in freshwater and marine environments. We show that the three thymidine kinase (tk) genes present in all sequenced nudivirus genomes, thus far, were absent in the Crangon crangon nudivirus, suggesting there are twenty-eight core genes shared by all nudiviruses. Furthermore, the phylogenetic data no longer support the subdivision of the family Nudiviridae into four genera (Alphanudivirus to Deltanudivirus), as recently adopted by the International Committee on Taxonomy of Viruses (ICTV), but rather shows two main branches of the family that are further subdivided. Our data support a recent proposal to create two subfamilies within the family Nudiviridae, each subdivided into several genera.

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Section: Resultsmentioning

confidence: 99%

Identification and Full Characterisation of Two Novel Crustacean Infecting Members of the Family Nudiviridae Provides Support for Two Subfamilies

Bateman

Kerr

Stentiford

et al. 2021

Viruses

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“…ODV-E66 protein, which has a chondroitin AC/alginate lyase (IPR008929) domain, is potentially involved in viral envelope formation and facilitates viral protein movement during infection by degrading larval peritrophic membrane in baculoviruses (34). This protein has been reported from baculoviruses and other nudiviruses (such as Homarus gammarus nudivirus and Penaeus monodon nudivirus), and it has been suggested that ODV-E66 is a per os infectivity factor (3,35).…”

Section: Limited Evidence Of Ornv Gene Expression Variation Between Chronically Infected Populations Principal-component Analysis Of Ornvmentioning

confidence: 99%

Transcription Profile and Genomic Variations of Oryctes Rhinoceros Nudivirus in Coconut Rhinoceros Beetles

Etebari

Parry

Beltran

et al. 2020

J Virol

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Oryctes rhinoceros nudivirus (OrNV) is a dsDNA virus which has been used as a biocontrol agent to suppress Coconut Rhinoceros Beetle (Oryctes rhinoceros) in Southeast Asia and the Pacific Islands. Recently a new wave of O. rhinoceros incursions in Oceania is thought to be related to the presence of low virulence isolates of OrNV or virus tolerant haplotypes of beetles. Here, chronically infected beetles were collected from the Philippines, Fiji, PNG and the Solomon Islands (SI). RNAseq was performed to investigate the global viral gene expression profiles and comparative genomic analysis of structural variations. Maximum likelihood phylogenic analysis indicated that OrNV strains from the SI and the Philippines are closely related to while OrNV strains from PNG and Fiji formed a distinct adjacent clade. We detected several polymorphic sites with a frequency higher than 35% in 892 positions of the viral genome. Non-synonymous mutations were detected in several hypothetical proteins, and 15 nudivirus core genes such as gp034, lef-8, lef-4 and vp91. We found limited evidence of variation in viral gene expression among geographic populations. Only a few genes such as gp01, gp022 and gp107 were differentially expressed among different strains. Additionally, small RNA sequencing from SI population suggests that OrNV is targeted by the host RNA interference (RNAi) response with abundant 21nt small RNAs. Some of these genomic changes are specific to the geographic population and could be related to particular phenotypic characteristics of the strain, such as viral pathogenicity or transmissibility, and this requires further investigation. IMPORTANCE Oryctes rhinoceros nudivirus has been an effective biocontrol agent against Coconut Rhinoceros Beetle in Southeast Asia and the Pacific Islands for decades. The recent outbreak of these beetles in many south pacific islands has a significant impact on livelihoods in the region. It has been suggested that this resurgence and spread of the pest is related to the presence of low virulence isolates of OrNV or virus tolerant haplotypes of beetles. We examined viral genomic and transcriptional variations in chronically infected beetles from different geographical populations. The high number of polymorphic sites among several geographical strains of OrNV were identified, but potentially only a few of these variations in the genome are involved in functional changes and can potentially alter their typical function. These findings provide valuable resources for future studies to improve our understanding of the OrNV genetic variation in different geographic regions and their potential link to virus pathogenicity.

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“…This protein is probably involved in ODV binding as peptide derivatives of this protein have affinity for the midgut epithelium (Sparks et al, 2011b, Xiang et al, 2011) (See table 1 for further information). This protein is was recently also shown to be important for the degradation of the peritrophic membrane (Hou et al, 2019). proteins; PIF5, 6, 8 and 9.…”

Section: Selection Of Pif Proteins For Co-immunoprecipitation Experimmentioning

confidence: 98%

“…The identified proteins were categorized according to their reported function into five different groups: per os infectivity, encircled with dark red; nucleocapsid assembly and actin rearrangement, encircled with blue; virion production, encircled with dark green; OB formation, encircled with purple; and rest, encircled with black. Sparks et al, 2011b, Xiang et al, 2011Hou et al, 2019 Polyhedron and BV associated, binds chitin, facilitate PM-passage, fusogenic properties Vialard et al, 1990, Li et al, 2003, Wang et al, 2010, Liu et al, 2011, Liu et al, 2019 ac114 Associated with BVs and ODVs; involved in embedding of ODVs in protein matrix Braunagel et al, 2003, Wang et al, 2010 2012 Nucleocapsid assembly / actin rearrangement PP78/83 ac9 Essential gene in Group I and II lepidopteran NPVs, WASP-like protein for actin nuclear assembly in association with Ac101, Ac102 and ODV-EC27 Vialard et al, 1993, Goley et al, 2006, Ohkawa et al, 2010 V-UBI ac35 Might inhibit degradation of shortlived viral proteins; important for BV production by facilitating nuclear egress of nucleocapsids in association with AC66 and AC141 Haas et al, 1996, Biwas et al, 2018 AC54 ac54 Core gene, associated with BVs and ODVs, Nucleocapsid assembly Olszweski et al, 1997a, Guan et al, 2016 ac100 Core gene, DNA binding protein, nucleocapsid assembly Wang et al, 2010b, Liu et al, 2012, Lai et al, 2018 ac101 Core gene, capsid associated, form a complex with AC102, PP78/83 and ODV-EC27, regulated by miR-3 Braunagel et al, 2001, Garavaglia et al, 2012, Hepp et al, 2018, Jiao et al, 2019 ac102 Associated with the nucleocapsids, involved in nuclearization of G-actin with AC101, PP78/83 and ODV-EC27 Hepp et al, 2018 VP80 ac104 Capsid associated; the nucleocapsids stick in the virogenic stroma in absence of Vp80 Braunagel et al, 2003…”

Section: Selection Of Pif Proteins For Co-immunoprecipitation Experimmentioning

confidence: 99%

“…2), as the infectivity of PIF8 mutant ODVs was partially rescued when damaging this membrane with calcofluor white (Zhu et al, 2013). PIF5 interaction partner ODV-E66 has recently also been shown to be involved in this as well (Hou et al, 2019). Once the ODVs have passed the peritrophic membrane, the proteolytically activated PIF0…”

Section: Comparison Of the Odv Entry Complex With Entry Complexes Of mentioning

confidence: 99%

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Baculovirus per os infectivity : a complex matter

Boogaard¹

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Baculoviruses are arthropod-specific large DNA viruses that orally infect the larvae of more than six hundred different insect species of the orders Lepidoptera, Hymenoptera and Diptera. Insect larvae become infected when they eat a food source that is contaminated with viral occlusion bodies (OBs). These OBs contain occlusion-derived viruses (ODVs), which are released upon ingestion of the OBs and infect the endothelial midgut cells. At least nine different ODV envelope proteins are essential for this oral infectivity and these are denoted as per os infectivity factors (PIFs). Seven of these PIFs form a complex, consisting of PIF1, 2, 3 and 4 that form a stable core complex, and PIF0 (P74), PIF6 and PIF8 (P95) that associate with this complex with lower affinity than the core components. The existence of a PIF complex and the fact that the pif genes are conserved in baculovirus genomes suggests that PIF-proteins cooperatively mediate oral infectivity rather than as individual functional entities. This review therefore discusses the knowledge obtained for individual PIFs in light of their relation with other members of the PIF complex.

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Baculovirus ODV-E66 degrades larval peritrophic membrane to facilitate baculovirus oral infection

Cited by 16 publications

References 52 publications

Identification and Full Characterisation of Two Novel Crustacean Infecting Members of the Family Nudiviridae Provides Support for Two Subfamilies

Identification and Full Characterisation of Two Novel Crustacean Infecting Members of the Family Nudiviridae Provides Support for Two Subfamilies

Transcription Profile and Genomic Variations of Oryctes Rhinoceros Nudivirus in Coconut Rhinoceros Beetles

Baculovirus per os infectivity : a complex matter

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