Baillon, emerging clinical yeasts

Batra, Roma; Boekhout, Teun; Guého, E.; Cabañes, F. Javier; Dawson, Thomas L.; Gupta, Aditya K.

doi:10.1016/j.femsyr.2005.05.006

Cited by 130 publications

(105 citation statements)

References 98 publications

(181 reference statements)

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“…Seven more Malassezia spp. (Malassezia dermatis, Malassezia equina, Malassezia japonica, Malassezia nana, Malassezia yamatoensis, Malassezia caprae, and Malassezia cuniculi) were described later by different groups (3)(4)(5)(6)(7)(8)(9). Of these, M. caprae, M. equina, M. nana, and M. cuniculi have been isolated only from domestic animals; the host specificity of these species prompted other workers to look for new species in the genus (10).…”

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confidence: 99%

Malassezia arunalokei sp. nov., a Novel Yeast Species Isolated from Seborrheic Dermatitis Patients and Healthy Individuals from India

et al. 2016

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The majority of species within the genus Malassezia are lipophilic yeasts that colonize the skin of warm-blooded animals. Two species, Malassezia globosa and Malassezia restricta, are implicated in the causation of seborrheic dermatitis/dandruff (SD/D). During our survey of SD/D cases, we isolated several species of Malassezia and noticed vast variations within a few lipid-dependent species. Variations observed in the phenotypic characteristics (colony morphology, absence of catalase activity, growth at 37°C, and precipitation surrounding wells containing Tween 20 or Cremophor EL) suggested the possible presence of a novel species. Sequence divergence observed in the internal transcribed spacer (ITS) region, the D1/D2 domain, and the intergenic spacer 1 (IGS1) region of rDNA and the TEF1 gene, PCR-restriction fragment length polymorphism (RFLP) analysis of the ITS2 region, and fluorescent amplified fragment length polymorphism analysis support the existence of a novel species. Based on phenotypic and molecular characterization of these strains, we propose a new species, namely, M. arunalokei sp. nov., and we designate NCCPF 127130 ‫؍(‬ MTCC 12054 ‫؍‬ CBS 13387) as the type strain. Malassezia spp. are lipophilic, unipolar budding, commensal yeasts that inhabit body surfaces rich in sebaceous glands in warm-blooded animals. In humans, these microorganisms have been implicated in a variety of skin diseases, including pityriasis versicolor (PV), seborrheic dermatitis/dandruff (SD/D), pityrosporum folliculitis (PF), and some subsets of psoriasis and atopic dermatitis (AD) (1). Weather conditions (such as temperature and humidity), the cutaneous microenvironment, or host defense mechanisms may predispose individuals to Malassezia infection or colonization. After a thorough analysis of morphological and biochemical characteristics, Gueho et al. described seven species (Malassezia furfur, Malassezia obtusa, Malassezia globosa, Malassezia slooffiae, Malassezia sympodialis, Malassezia pachydermatis, and Malassezia restricta) (2). Seven more Malassezia spp. (Malassezia dermatis, Malassezia equina, Malassezia japonica, Malassezia nana, Malassezia yamatoensis, Malassezia caprae, and Malassezia cuniculi) were described later by different groups (3-9). Of these, M. caprae, M. equina, M. nana, and M. cuniculi have been isolated only from domestic animals; the host specificity of these species prompted other workers to look for new species in the genus (10).In human infections, certain species possibly play important roles in pathogenesis. M. globosa is commonly associated with SD/D (11). Other species, including M. restricta, M. furfur, M. sympodialis, M. obtusa, and M. slooffiae, are occasionally associated with SD/D at different geographical locations (12). SD/D is widely prevalent in India, and M. globosa and M. restricta are commonly associated with this condition (13,14). While conducting a detailed survey of SD/D cases, however, we noticed vast variations within a few lipid-dependent strains of Malassezia, which are chara...

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Malassezia arunalokei sp. nov., a Novel Yeast Species Isolated from Seborrheic Dermatitis Patients and Healthy Individuals from India

et al. 2016

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“…However, difficulties in cultivating Malassezia organisms may limit the analyses and bias the observations (Batra et al, 2005). Thus, molecular approaches, particularly analyses of ribosomal genes and internal transcribed regions, have been used for detection, identification, and characterization of Malassezia species (Cafarchia et al, 2011).…”

Section: Biological Cultural and Immunological Characteristicsmentioning

confidence: 99%

Psoriasis and Malassezia Yeasts

Prohić¹

2012

Psoriasis - A Systemic Disease

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“…All members of the genus Malassezia, with the exception of Malassezia pachydermatis, show a unique lipid dependency (Batra et al, 2005;Midgley, 2000). As a result, the fungi produce lipolytic enzymes, such as lipases, to digest triacylglycerides (TAGs) contained in the sebum and take up the free fatty acids.…”

Section: Introductionmentioning

confidence: 99%

Secreted lipases from Malassezia globosa: recombinant expression and determination of their substrate specificities

et al. 2016

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Malassezia globosa, which is associated with skin conditions such as dandruff and seborrhoeic dermatitis, possesses 13 secreted lipases, but only MgLip1, MgMDL2 and MgLip2 have been characterized. To understand the substrate preferences of these lipases and by extension their potential role in colonizing human skin, we expressed all 13 predicted secreted lipases in Pichia pastoris and evaluated their ability to utilize mono-, di-and triolein substrates. The M. globosa family class 3 lipases were shown to be specific for mono-and diacylglycerols, but exhibited no regio-selective production of diacylglycerols, which are of special interest for industrial applications. Lipases belonging to the Lip family utilized all substrates. In a further step, five lipases previously demonstrated to be expressed on human skin were tested against the eight most common di-and triacylglycerols in human sebum. All lipases liberated free fatty acids from three to eight of these substrates, proving their ability to hydrolyse key components of human sebum. Again, only Lip family lipases showed activity on triacylglycerides. Based on the demonstrated activity and expression levels of MgLip2 in M. globosa, the Lip lipase family appears to have the highest impact for the pathogenicity of M. globosa.

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Baillon, emerging clinical yeasts

Cited by 130 publications

References 98 publications

Malassezia arunalokei sp. nov., a Novel Yeast Species Isolated from Seborrheic Dermatitis Patients and Healthy Individuals from India

Malassezia arunalokei sp. nov., a Novel Yeast Species Isolated from Seborrheic Dermatitis Patients and Healthy Individuals from India

Psoriasis and Malassezia Yeasts

Secreted lipases from Malassezia globosa: recombinant expression and determination of their substrate specificities

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