Bakkenolides from<i>Petasites tricholobus</i>and Their Neuroprotective Effects Related to Antioxidant Activities

Wang, Yuliang; Li, Runping; Guo, Meili; Zhang, Ge; Zhang, Na; Ma, Yanli

doi:10.1055/s-0028-1088377

Cited by 28 publications

(20 citation statements)

References 13 publications

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“…The concentrations were selected according to the works of Wang et al (2009). In this study, ZBN was used in eight distinct doses at concentrations of 10,25,50,75,100,150,200 and 400 mg L -1 .…”

Section: Healthy Neuron Cell Culturesmentioning

confidence: 99%

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

et al. 2014

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The main objective of this study is to investigate the cytotoxic, genotoxic and antioxidant properties of zingiberene (ZBN) in an in vitro rat brain cell culture study. The cytotoxic effect was determined against the rat neuron and N2a neuroblastoma (N2a-NB) cell lines using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, while the antioxidant activity was assessed using the total antioxidant capacity (TAC) and total oxidative stress (TOS) assays. The effects on DNA damage were also evaluated in this study by the single cell gel electrophoresis assay. The results indicated that ZBN has an anti-proliferative activity suppressing the proliferation of N2a-NB cells at concentrations over 50 mg L -1 and neuron cells at concentrations over 150 mg L -1. In addition, ZBN treatments at higher doses (B50 mg L -1 ) led to increases of TOS levels in N2a-NB cell cultures. However 25 mg L -1 of ZBN treatment caused increases of TAC levels in cultured neuron and N2a-NB cell cultures while ZBN at doses of 10-400 mg L -1 did not increase the number of total damage score in both cell lines. This study clearly indicates that ZBN has a significant potential to be used as a natural anticancer agent in cultured N2a-NBs.

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Section: Healthy Neuron Cell Culturesmentioning

confidence: 99%

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

et al. 2014

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“…α-COP was applied into cultures at concentrations of 10,25,50,75,100,150,200 and 400 mg/L for 24 h. The concentrations were selected according to the work of Wang et al (2009).…”

Section: Cell Culturesmentioning

confidence: 99%

Cytotoxic and cytogenetic effects of α-copaene on rat neuron and N2a neuroblastoma cell lines

et al. 2014

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Alpha-copaene (α-COP), a tricyclic sesquiterpene, is present in several essential oils of medicinal and aromatic plants and has antioxidant and antigenotoxic features. Its cytotoxic, cytogenetic and oxidative effects have not been investigated in neuron and N2a neuroblastoma (NB) cell cultures. Therefore, we aimed to describe in vitro: (i) cytotoxic properties by 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenlytetrazolium bromide test; (ii) antioxidant/oxidant activity by total antioxidant capacity (TAC) and total oxidative status (TOS) analysis; and (iii) genotoxic damage potential by single cell gel electrophoresis -of α-COP in healthy neuron and N2a-NB cell cultures for the first time. Significant (P < 0.05) decrease in cell proliferation were observed in cultured primary rat neurons starting with the concentration of 150 mg/L and in N2a-NB cells starting with 100 mg/L. In addition, 25 mg/L of α-COP treatment caused increase of TAC levels and α-COP treatments at higher doses led to increase of TOS levels in neuron N2a-NB cell cultures. Moreover, none of the tested concentrations of α-COP have shown a genotoxic effect on both cell lines. Our findings clearly demonstrate that α-COP exhibited mild cytotoxic effects on N2a-NB cell line. In conclusion, α-COP may have potential as an anticancer agent, which needs to be further studied.

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“…tatewakianus (Dong et al ., ) and P . tricholobus (Wang et al ., ). Thus, the potential bioactivity of BD may represent the medical activity of these Petasites plants and their extracts to some degree.…”

Section: Introductionmentioning

confidence: 97%

“…japonicas (Shirahata et al ., ), P . tricholobus (Wang et al ., ; Zhang et al ., ) and P . tatewakianus (Dong et al ., ).…”

Section: Introductionmentioning

confidence: 99%

LC‐MS/MS determination of bakkenolide D in rats plasma and its application in pharmacokinetic studies

Dai

Pei

Tang

et al. 2013

Biomedical Chromatography

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A sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for determination of bakkenolide D (BD), which was further applied to assess the pharmacokinetics of BD. In the LC-MS/MS method, the multiple reaction monitoring mode was used and columbianadin was chosen as internal standard. The method was validated over the range of 1-800 ng/mL with a determination coefficient >0.999. The lower limit of quantification was 1 ng/mL in plasma. The intra- and inter-day accuracies for BD were 91-113 and 100-104%, respectively, and the inter-day precision was <15%. After a single oral dose of 10 mg/kg of BD, the mean peak plasma concentration of BD was 10.1 ± 9.8 ng/mL at 2 h. The area under the plasma concentration-time curve (AUC0-24 h ) was 72.1 ± 8.59 h ng/mL, and the elimination half-life (T1/2 ) was 11.8 ± 1.9 h. In case of intravenous administration of BD at a dosage of 1 mg/kg, the AUC0-24 h was 281 ± 98.4 h⋅ng/mL, and the T1/2 was 8.79 ± 0.63 h. Based on these results, the oral bioavailability of BD in rats at 10 mg/kg is 2.57%.

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Bakkenolides fromPetasites tricholobusand Their Neuroprotective Effects Related to Antioxidant Activities

Cited by 28 publications

References 13 publications

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

Cytotoxic and cytogenetic effects of α-copaene on rat neuron and N2a neuroblastoma cell lines

LC‐MS/MS determination of bakkenolide D in rats plasma and its application in pharmacokinetic studies

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