Basic fibroblast growth factor adsorption and release properties of calcium phosphate

Abstract

Basic fibroblast growth factor (bFGF) is well known as a potent angiogenic factor playing a crucial role in wound-healing processes. Apatitic substrates such as hydroxyapatite and carbonated apatite (CA) could be potential carriers of growth factors because of their physicochemical similarities to bone mineral. These materials have been compared for their bFGF adsorption and release properties. The adsorption of the growth factor was higher on carbonated apatite than on hydroxyapatite, probably owing to environments with labile nonapatitic CO3(2-) and HPO4(2-) groups, along with a higher specific surface area which gives the CA a higher surface reactivity. These environments can be exchanged very rapidly, leading to the release of bFGF. The controlled release of adsorbed growth factor from carbonated apatite could provide means of improving bone healing in the future.

Synthetic bone mimetic matrix-mediated in situ bone tissue formation through host cell recruitment

“…Measurements of bFGF adsorption onto the mineralized materials suggest that a majority of bFGF was adsorbed by the graft (>80%). This is consistent with other studies that showed high levels of bFGF adsorption by the CaP-based materials [35, 36]. …”

Effect of phase composition and microstructure of calcium phosphate ceramic particles on protein adsorption

“…The microstructure of materials, including roughness, microporosity and SSA, is another factor affecting protein adsorption. Midy et al proved that the higher adsorption of basic fibroblast growth factor on carbonated apatite than HA could be ascribed to the higher SSA, which gave the carbonated apatite a higher surface reactivity [43]. Suh et al reported that the amount of BSA adsorbed on silicon particles increased significantly with increasing size of the micropores, and this effect was in addition to the contribution of SSA to BSA adsorption [44].…”

A hydroxyapatite coating covalently linked onto a silicone implant material

“…42 Further- more, intact HAp surfaces containing carbonate groups may be potential carriers of growth factors because of their physicochemical similarities to bone mineral. 43 The HAp-coated silicone material can, therefore, be assumed to be bioactive in vivo.…”