Batch and fed-batch production of butyric acid by Clostridium butyricum ZJUCB

He, Guoqing; Kong, Qing; Chen, Qihe; Ruan, Hui

doi:10.1631/jzus.2005.b1076

Cited by 60 publications

(33 citation statements)

References 15 publications

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“…The glucose and xylose concentrations were measured using Sugar Content Measuring Device (SBA-40C) and DNS method, respectively [21]. Quantitative analysis of butyric acid and acetic acid was performed by gas chromatography (Agilent 6820 GE, Agilent Technologies) [22]. …”

Section: Methodsmentioning

confidence: 99%

Production of Butyric Acid from Glucose and Xylose with Immobilized Cells of Clostridium tyrobutyricum in a Fibrous-bed Bioreactor

Jiang

Wang

Song

et al. 2008

Appl Biochem Biotechnol

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Butyric acid has many applications in chemical, food, and pharmaceutical industries. In the present study, Clostridium tyrobutyricum ATCC 25755 was immobilized in a fibrous-bed bioreactor to evaluate the performance of butyrate production from glucose and xylose. The results showed that the final concentration and yield of butyric acid were 13.70 and 0.46 g g(-1), respectively, in batch fermentation when 30 g L(-1) glucose was introduced into the bioreactor. Furthermore, high concentration 10.10 g L(-1) and yield 0.40 g g(-1) of butyric acid were obtained with 25 g L(-1) xylose as the carbon source. The immobilized cells of C. tyrobutyricum ensured similar productivity and yield from repeated batch fermentation. In the fed-batch fermentation, the final concentration of butyric acid was further improved to 24.88 g L(-1) with one suitable glucose feeding in the fibrous-bed bioreactor. C. tyrobutyricum immobilized in the fibrous-bed bioreactor would provide an economically viable fermentation process to convert the reducing sugars derived from plant biomass into the final bulk chemical (butyric acid).

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Section: Methodsmentioning

confidence: 99%

Production of Butyric Acid from Glucose and Xylose with Immobilized Cells of Clostridium tyrobutyricum in a Fibrous-bed Bioreactor

Jiang

Wang

Song

et al. 2008

Appl Biochem Biotechnol

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“…Batch, free-cell Glucose 6.30 [11] C. butyricum ZJUCB Batch, free-cell Glucose 12.25 [12] Fed-batch, free-cell 16.74…”

Section: Populetimentioning

confidence: 99%

“…C. tyrobutyricum ATCC 25755, a gram-positive, rodshaped, and anaerobic bacterium, produces butyric acid as the main product from various carbohydrates, such as glucose and xylose [10]. The recent progresses of butyric acid production are summarized in Table 1 [11][12][13][14][15][16][17][18][19][20][21]. Compared with other strains, the wild type C. tyrobutyricum produced higher level of butyric acid in the fedbatch free-cell fermentation, 24.88-33.00 g/L vs. 6.…”

Section: Introductionmentioning

confidence: 99%

High production of butyric acid by Clostridium tyrobutyricum mutant

Miller

McFann

et al. 2015

Front. Chem. Sci. Eng.

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The objective of this study was to improve the production of butyric acid by process optimization using the metabolically engineered mutant of Clostridium tyrobutyricum (PAK-Em). First, the free-cell fermentation at pH 6.0 produced butyric acid with concentration of 38.44 g/L and yield of 0.42 g/g. Second, the immobilizedcell fermentations using fibrous-bed bioreactor (FBB) were run at pHs of 5.0, 5.5, 6.0, 6.5 and 7.0 to optimize fermentation process and improve the butyric acid production. It was found that the highest titer of butyric acid, 63.02 g/L, was achieved at pH 6.5. Finally, the metabolic flux balance analysis was performed to investigate the carbon rebalance in C. tyrobutyricum. The results show both gene manipulation and fermentation pH change redistribute carbon between biomass, acetic acid and butyric acid. This study demonstrated that high butyric acid production could be obtained by integrating metabolic engineering and fermentation process optimization.

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“…The main field of application of butyric acid is the food industry. The dairy industry is using the pure acids; the esters are used as food additives to amplify the fragrance of tropic fruits (Centeno et al 2002;He et al 2005;Watson 2002). …”

Section: Sequence Chemical Products and Applications From Organic Acidsmentioning

confidence: 99%

Microbial Life on Green Biomass and Their Use for Production of Platform Chemicals

Schönicke

Shahab

Hamann

et al. 2014

Microorganisms in Biorefineries

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Batch and fed-batch production of butyric acid by Clostridium butyricum ZJUCB

Cited by 60 publications

References 15 publications

Production of Butyric Acid from Glucose and Xylose with Immobilized Cells of Clostridium tyrobutyricum in a Fibrous-bed Bioreactor

Production of Butyric Acid from Glucose and Xylose with Immobilized Cells of Clostridium tyrobutyricum in a Fibrous-bed Bioreactor

High production of butyric acid by Clostridium tyrobutyricum mutant

Microbial Life on Green Biomass and Their Use for Production of Platform Chemicals

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