BDNF mRNA expression and protein localization are changed in age-related hearing loss

Rüttiger, Lukas; Panford-Walsh, Rama; Schimmang, Thomas; Tan, Justin; Zimmermann, Ulrike; Rohbock, Karin; Köpschall, Iris; Limberger, Annette; Müller, Marcus; Fraenzer, Jürgen-Theodor; Cimerman, Jelka; Knipper, Marlies

doi:10.1016/j.neurobiolaging.2006.02.008

Cited by 46 publications

(40 citation statements)

References 79 publications

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“…Along the tonotopic axis, BDNF exon IV is expressed in a gradient, with increasing expression in the midbasal/basal turns of the cochlea (Schimmang et al, 2003;Rü ttiger et al, 2007). Using sequencespecific riboprobes, we confirmed the tonotopic distribution of BDNF in spiral ganglion neurons of the cochlea (Fig.…”

Section: Expression Of Bdnf and The Care Binding Proteins In Spiral Gsupporting

confidence: 62%

“…BDNF has a complex gene structure, and the nomenclature has been altered recently (Aid et al, 2007). Although we published previous work using the old nomenclature system (Rü ttiger et al, 2007;Tan et al, 2007), the present work adopts the system of Aid et al (2007).…”

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confidence: 99%

“…Trauma-induced up-regulation (R. Panford-Walsh, W. Singer, L. Rü ttiger, S. Hadjab, J. Tan, M. Geisler, U. Zimmerman, I. Köpschall, K. Röhbock, A. Vieljans, et al, submitted;Tan et al, 2007) and age-related decrease (Rü ttiger et al, 2007) of cochlear BDNF levels were discussed as a trigger for pathological plasticity responses in the central auditory system. As such, the elucidation of the mechanisms that participate in the traumainduced alteration of cochlear BDNF levels may be crucial for future therapeutic designs.…”

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confidence: 99%

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Salicylate Alters the Expression of Calcium Response Transcription Factor 1 in the Cochlea: Implications for Brain-Derived Neurotrophic Factor Transcriptional Regulation

Singer

Panford-Walsh²,

Watermann³

et al. 2008

Mol Pharmacol

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Brain-derived neurotrophic factor (BDNF) is a key neurotrophin whose expression is altered in response to neurological activity, influencing both short-and long-term synaptic changes. The BDNF gene consists of eight upstream exons (I-VII), each of which has a distinct promoter and can be independently spliced to the ninth coding exon (IX). We showed recently that the expression of BDNF exon IV in the cochlea is altered after exposure to salicylate, an ototoxic drug that in high doses is able to induce hearing loss and tinnitus. These changes were a crucial trigger for plasticity changes in the central auditory system. BDNF exon IV expression is regulated via interaction between calcium-response elements CaRE1, CaRE2, and CaRE3/Cre (CaREs) that are bound by the transcription factors CaRF1, upstream stimulatory factors 1 and 2 (USF1/2), and cAMP/Ca 2ϩ response element-binding protein (CREB), respectively. To determine whether the salicylate-induced changes in cochlear BDNF exon IV expression include a differential use of the CaRE binding proteins, we studied the level of the corresponding binding proteins in the spiral ganglion neurons before and after systemic application of concentrated salicylate using in situ hybridization and RT-PCR. BDNF exon IV and CaRF1 expression were up-regulated after application of salicylate, whereas USF1/2 and CREB mRNA expression remained unaffected. The changes in BDNF exon IV and CaRF1 expression were also dose-dependent. The data show Ca 2ϩ and CaRF1 as messengers of trauma (salicylate)-induced altered BDNF levels in the cochlea. Furthermore, they also provide the first evidence that a differential regulation of BDNF transcription factors might participate in BDNF-mediated plasticity changes.

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Section: Expression Of Bdnf and The Care Binding Proteins In Spiral Gsupporting

confidence: 62%

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confidence: 99%

mentioning

confidence: 99%

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Salicylate Alters the Expression of Calcium Response Transcription Factor 1 in the Cochlea: Implications for Brain-Derived Neurotrophic Factor Transcriptional Regulation

Singer

Panford-Walsh²,

Watermann³

et al. 2008

Mol Pharmacol

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“…Semi-quantitative RT-PCR was performed as described (Rüttiger et al, 2006). In brief, cochlear mRNA from P12 hypothyroid and control rats was isolated using the Dynabeads mRNA Direct Kit (Dynal) and reverse-transcribed with Superscript II TH-dependent Kcnq4 and prestin expression in OHCs (Invitrogen).…”

Section: Semi-quantitative Rt-pcrmentioning

confidence: 99%

Thyroid hormone receptors TRα1 and TRβ differentially regulate gene expression ofKcnq4and prestin during final differentiation of outer hair cells

Winter

Braig

Zimmermann

et al. 2006

Journal of Cell Science

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“…Thus, decreasing EP was associated with a loss or shrinkage of large diameter auditory nerve fibre dendrites and a reduction of the size of spiral ganglion cells. Rüttiger et al (2007) found an age dependent reduction of BDNF mRNA expression in high frequency spiral ganglion cells. In contrast, BDNF protein expression was preserved in the cochlear ganglion cells of old gerbils but declined in their central and peripheral processes.…”

Section: Spiral Ganglion Cells and Auditory Dendritesmentioning

confidence: 92%

The Mongolian Gerbil as a Model for the Analysis of Peripheral and Central Age-Dependent Hearing Loss

Gleich¹,

Strutz²

2012

Hearing Loss

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BDNF mRNA expression and protein localization are changed in age-related hearing loss

Cited by 46 publications

References 79 publications

Salicylate Alters the Expression of Calcium Response Transcription Factor 1 in the Cochlea: Implications for Brain-Derived Neurotrophic Factor Transcriptional Regulation

Salicylate Alters the Expression of Calcium Response Transcription Factor 1 in the Cochlea: Implications for Brain-Derived Neurotrophic Factor Transcriptional Regulation

Thyroid hormone receptors TRα1 and TRβ differentially regulate gene expression ofKcnq4and prestin during final differentiation of outer hair cells

The Mongolian Gerbil as a Model for the Analysis of Peripheral and Central Age-Dependent Hearing Loss

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