BdorOBP83a-2 Mediates Responses of the Oriental Fruit Fly to Semiochemicals

Wu, Zhongzhen; Lin, Jintian; Zhang, He; Zeng, Xinnian

doi:10.3389/fphys.2016.00452

Cited by 42 publications

(42 citation statements)

References 71 publications

(96 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The associated EAG response of the antennae toward the tested attractant (methyl eugenol) decreased significantly when Bdorobp2 was silenced in both male and female insects (Figure 6). Our observation is consistent with the previous reports that reduction of vital, antennaespecific OBP transcript levels can result in a decrease in EAG response for our insect, B. dorsalis (Wu et al, 2016). In our experiments, both the male and female B. dorsalis adults show a significantly decreased electrophysiological response to the olfactory stimulation with the attractant odor, methyl eugenol, after dsRNA injection.…”

Section: Discussionsupporting

confidence: 93%

An Antennae-Specific Odorant-Binding Protein Is Involved in Bactrocera dorsalis Olfaction

Zhao

Liang

et al. 2020

Front. Ecol. Evol.

View full text Add to dashboard Cite

Insect antennae are important olfactory organs that house high concentrations of odorant-binding proteins (OBPs) in the sensillum lymph. Previous studies in other insects have shown that OBPs play important roles in transporting odorants and enhancing the sensitivity of the olfactory system. However, the functions of OBPs in the oriental fruit fly, Bactrocera dorsalis, especially those specifically expressed in antennae, have not been fully elucidated. In this study, cDNA libraries were constructed from both the male and female antennal transcriptome, and twenty OBPs were identified in total. The expression profiles of these OBPs were examined in the adult antenna, head, thorax, leg, and abdomen of both sexes. Seven of the identified OBP genes had significantly higher expression in both the male and female antennae than in other tissues, while the transcript levels of the remaining OBPs varied across different tissues. Regarding the function of antenna-specific OBPs, we targeted Bdorsobp2 as a representative for further RNA interference (RNAi) and identified via electrophysiology a decrease in detection of a potential species-specific a potent attractant, methyl eugenol. Moreover, subsequent behavioral assay data showed that the behavioral response of B. dorsalis toward this odorant decreased when Bdorobp2 was silenced with injection of double-stranded RNA (dsRNA). Combined, these results support our initial hypothesis that antennae-specific OBPs are of critical importance for insect odorant detection, sensitivity, and behavior.

show abstract

Section: Discussionsupporting

confidence: 93%

An Antennae-Specific Odorant-Binding Protein Is Involved in Bactrocera dorsalis Olfaction

Zhao

Liang

et al. 2020

Front. Ecol. Evol.

View full text Add to dashboard Cite

show abstract

“…BdOrco was reportedly up-regulated by the sexual attractant ME and played a critical role in mediating the taxis of B. dorsalis males to ME 14 . Furthermore, gene silencing via the microinjection of dsRNA recently confirmed that BdorOBP83a-2 actively participates in the process of ME detection by B. dorsalis 33 . The phylogenetic tree was generated to show how BdorOBP2 and BdorOBP83a-2 belong to different groups of orthologous proteins (Fig.…”

Section: Discussionmentioning

confidence: 90%

“… 18 studied 25 semiochemicals’ binding potential to a GOBP of B. dorsalis for predicting and screening behaviorally active compounds. Silencing Orco and OBP83a-2 decreased the ability of B. dorsalis adults to find semiochemical lures in a timely manner 14 , 33 . In this regard, this unique research strategy may help chemical ecologists in their prospecting of active semiochemicals for eco-friendly use in pest management practices.…”

Section: Introductionmentioning

confidence: 96%

BdorOBP2 plays an indispensable role in the perception of methyl eugenol by mature males of Bactrocera dorsalis (Hendel)

Liu

Zhao

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Bactrocera dorsalis (Hendel) is a fruit-eating pest that causes substantial economic damage to the fresh produce industry in tropical and sub-tropical countries. Methyl eugenol (ME) is a powerful attractant for mature males of B. dorsalis, and has been widely used for detecting, luring and eradicating B. dorsalis populations worldwide. However, the molecular mechanism underlying the olfactory perception of ME remains largely unknown. Here, we analyzed the differential proteomics profiling of the antennae between ME-responsive and ME-non-responsive males by using isobaric tags for relative and absolute quantitation (iTRAQ). In total, 4622 proteins were identified, of which 277 proteins were significant differentially expressed, with 192 up-regulated and 85 down-regulated in responsive male antennae. Quantitative real-time PCR (qRT-PCR) analysis confirmed the authenticity and accuracy of the proteomic analysis. Based on the iTRAQ and qRT-PCR results, we found that the odorant-binding protein 2 (BdorOBP2) was abundantly expressed in responsive male antennae. Moreover, BdorOBP2 was significantly up-regulated by ME in male antennae. Mature males showed significantly greater taxis toward ME than did mature females. Silencing BdorOBP2 reduced mature males’ responsiveness to ME. These results indicate that BdorOBP2 may play an essential role in the molecular mechanism underlying B. dorsalis olfactory perception of ME.

show abstract

“…In B. dorsalis , chemosensory perception plays a key role in behavior regulation such as, host-seeking, mating and oviposition. It also exhibits remarkable developmental phases in olfactory behaviors (Wu et al, 2016 ). In this study, we examined the de novo transcriptome of B. dorsalis and identified 21 IR, 12 GR, and 43 OR genes.…”

Section: Introductionmentioning

confidence: 99%

Olfactory Plasticity: Variation in the Expression of Chemosensory Receptors in Bactrocera dorsalis in Different Physiological States

Jin

Zhou

et al. 2017

Front. Physiol.

View full text Add to dashboard Cite

Changes in physiological conditions could influence the perception of external odors, which is important for the reproduction and survival of insect. With the alteration of physiological conditions, such as, age, feeding state, circadian rhythm, and mating status, insect can modulate their olfactory systems accordingly. Ionotropic, gustatory, and odorant receptors (IR, GR, and ORs) are important elements of the insect chemosensory system, which enable insects to detect various external stimuli. In this study, we investigated the changes in these receptors at the mRNA level in Bactrocera dorsalis in different physiological states. We performed transcriptome analysis to identify chemosensory receptors: 21 IRs, 12 GRs, and 43 ORs were identified from B. dorsalis antennae, including almost all previously known chemoreceptors in B. dorsalis and a few more. Quantitative real-time polymerase chain reaction analysis revealed the effects of feeding state, mating status and time of day on the expression of IR, GR, and OR genes. The results showed that expression of chemosensory receptors changed in response to different physiological states, and these changes were completely different for different types of receptors and between male and female flies. Our study suggests that the expressions of chemosensory receptors change to adapt to different physiological states, which may indicate the significant role of these receptors in such physiological processes.

show abstract

BdorOBP83a-2 Mediates Responses of the Oriental Fruit Fly to Semiochemicals

Cited by 42 publications

References 71 publications

An Antennae-Specific Odorant-Binding Protein Is Involved in Bactrocera dorsalis Olfaction

An Antennae-Specific Odorant-Binding Protein Is Involved in Bactrocera dorsalis Olfaction

BdorOBP2 plays an indispensable role in the perception of methyl eugenol by mature males of Bactrocera dorsalis (Hendel)

Olfactory Plasticity: Variation in the Expression of Chemosensory Receptors in Bactrocera dorsalis in Different Physiological States

Contact Info

Product

Resources

About