Beban Candida dalam Saluran Cerna Anak

Abstract

AbstrakMikrobiota terbesar pada tubuh manusia terdapat pada saluran cerna (70%).Mikrobiota normal saluran cerna tersusun atas komposisi mikroorganisme yang unik dan berada dalam keadaan seimbang, didalamnya termasuk Candidaspp., yang hidup sebagai komensal. Data tentang keberadaan Candida dalam usus terutama berasal dari orang dewasa, sedangkan data pada anak sangat jarang. Penelitian ini bertujuan untuk mengetahui keragaman spesies Candida dan bebannya pada saluran cerna anak. Untuk menghitung beban jamur, sebanyak 0,2 g tinja ditanam pada agar sabouraud dekstrosa (ASD). Beban jamur dihitung berdasarkan koloni yang tumbuh dan disesuaikan menjadi per gram tinja (colony forming unit – CFU/g tinja). Identifikasi Candida dilakukan berdasarkan koloni yang tumbuh pada medium CHROMagar Candida (Paris, France). Populasi penelitian adalah anak umur 0-18 tahun (sesuai kriteria WHO). Sampel berasal dari RT 10 dan 11, Kampung Pluis, Jakarta Selatan dan RT 4, 6 dan 9, Kelurahan Cawang. Sebanyak 61 sampel tinja ditanam pada medium, dan didapat 72 isolat dari 43 pertumbuhan yang berasal dari 54 anak sehat dan tujuh anak sakit, yang dirawat di Departemen Kesehatan Anak RS UKI dengan berbagai sebab. Isolat yang didapat terdiri atas Candida tropicalis, Candida albicans, Candida parapsilosis, dan Candida glabrata. Beban jamur 1-50 CFU ditemukan pada 18 pasien, diikuti oleh beban jamur 151-200 CFU pada tujuh pasien. Selain itu, ditemukan infeksi campuran (dua spesies) pada sembilan sampel dan 3 spesies pada tujuh sampel. Tinja anak sakit didominasi C. tropicalis, empat sampel memiliki campuran C. tropicalis dengan C. albicans, dan C. tropicalis dengan C. parapsilosis.
 Kata Kunci: Candida, spesies, CFU, beban jamurCandida Load in the Children’s Gastrointestinal TractAbstractThe largest microbiota in the human body is found in the digestive tract (70%). The microbiota in the digestive tract is normally composed of a unique composition of microorganisms and is in a balanced state, including Candida spp., which live as commensals. Data on the presence of Candida in the intestine, especially coming from an adult, while the data in children are very rare. This study aims to determine the diversity of Candida species and fungal burden on the children’s digestive tract. To calculate the fungal load, 0.2g of faeces were planted on sabouraud dextrose agar (ASD). The fungal load was calculated based on the growing colonies and adjusted to be per gram of faeces (colony forming unit - CFU/g of faeces). Candida identification was carried out based on the colonies that grew on the CHROMagar Candida medium (Paris, France). The study population was children aged 0-18 years (according to WHO criteria). Samples came from RT 10 and 11, KampungPluis, South Jakarta and RT 4, 6 and 9, Cawang. A total of 61 stool samples were planted on the medium, and obtained 72 isolates from 43 growths from 54 healthy children and seven children who were treated at the Children’s Health Department of UKI Hospital for various reasons. The isolates obtained consisted of Candida tropicalis, Candida albicans, Candida parapsilosis, and Candida glabrata. 1-50 CFU fungal burden was found in 18 patients, followed by 151-200 CFU fungal burden in seven patients. In addition, there were mixed infections (two species) in nine samples and three species in seven samples. The stool of the sick children was predominantly C. tropicalis, four samples had a mixture of C. tropicalis with C. albicans, and C. tropicaliswith C. parapsilosis.Keywords: Candida, species, CFU, fungal burden

Influence of the Environment on Biofilm Formation Candida albicans of Vulvovaginal Candidiasis Isolate Patient

“…C. albicans is a species pathogen that will proliferate at temperatures between 25-37 °C. 29 The C. albicans detachment process, which is essential for biofilm formation, is also thought to occur before the biofilm formation process. 6,18 Solvent plays an essential function in this research as a biocatalyst, such as substrate biotransformation or carrier substrate phase, making it easier to observe biofilm formation outcomes.…”

The Cardioprotective Effect of Polysaccharide Sulphate Isolated from Brown Algae (Sargassum polycystum)

“…These results indicate that the treatment groups that were given sulfated polysaccharides or simvastatin were able to decrease triglyceride levels. This is because in addition to increasing HDL levels and reducing total cholesterol levels in the blood, simvastatin can also reduce triglyceride levels [30]. The same held true for the sulfated polysaccharides [22,31].…”

“…These results indicate that the treatment groups that were given simvastatin and sulfated polysaccharides had an effect in the form of a decrease in total cholesterol levels. This is because simvastatin is a statin class drug which is the first line of defence in reducing total cholesterol levels in the blood [30]. Regarding the previous research, it is showed that sulfated polysaccharides have a better effect than simvastatin in reducing total cholesterol levels [22,31].…”

“…These results indicate that the treatment groups that were given sulfated polysaccharides or simvastatin experienced an increase in HDL levels. This is because simvastatin, in addition to lowering total cholesterol levels, can also increase HDL levels in the blood [30]. As for sulfated polysaccharides, besides having a better effect than simvastatin in reducing total cholesterol levels, sulfated polysaccharides are also able to increase HDL levels [22,31].…”