Behavioural toxicity assessment of silver ions and nanoparticles on zebrafish using a locomotion profiling approach

Ašmonaitė, Giedrė; Boyer, Scott; Souza, Karine Bresolin de; Wassmur, Britt; Sturve, Joachim

doi:10.1016/j.aquatox.2016.01.013

Cited by 69 publications

(34 citation statements)

References 41 publications

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“…AgNPs' toxicity can accumulate in several organs, thereby causing toxicity, in part via oxidative stress including pulmonary and germ cell toxicity, embryo toxicity, and cell death. [2][3][4][5][6][7][8][9] Nanoparticle-induced oxidative stress is hypothesized to be the main mechanism regulating the biological effects of these nanoparticles. 10,11 Toxicity of AgNPs could also be due to release of Ag ions in the system or extensive systemic distribution of Ag in tissues causing oxidative stress, protein or DNA damage, and apoptotic cell death.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and hepatoprotective role of selenium against silver nanoparticles

Ansar

Alshehri

Abudawood

et al. 2017

IJN

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Silver nanoparticles (AgNPs) have attracted the most interest in terms of their potential biomedical and industrial applications. However, these nanoparticles have shown their toxic behavior toward environment, living tissues, and organisms. Selenium (Se), an essential trace element, is necessary for various metabolic processes, including protection against oxidative stress and immune function. The present study was undertaken to evaluate the effect of Se against AgNP-induced hepatic oxidative stress. AgNPs were synthesized and then prepared nanoparticles were characterized using various analytical techniques such as UV-visible spectroscopy, X-ray diffraction, and transmission electron microscopy. Rats were administered AgNPs intraperitoneally (5 mg/kg/day) and Se (0.2 mg/kg) was given by gavage. AgNP administration induced hepatic damage as indicated by the serum marker enzymes with reduction in levels of glutathione, and decrease in activities of SOD, CAT, and GSH-peroxidase ( P <0.05). Decrease in levels of total antioxidant capacity (TAC) and increase in level of C-reactive protein (CRP) was also observed in AgNP-treated group compared to control group. However, Se markedly attenuated AgNP-induced biochemical alterations, levels of TAC, CRP, and serum transaminases (AST, ALT) ( P <0.05). Taken together, these findings suggest that administration of AgNPs produces hepatotoxicity in rats, whereas Se supplementation attenuates these effects.

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Section: Introductionmentioning

confidence: 99%

Antioxidant and hepatoprotective role of selenium against silver nanoparticles

Ansar

Alshehri

Abudawood

et al. 2017

IJN

View full text Add to dashboard Cite

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“…These two properties have allowed using these NPsin vaccines and bactericidal compounds in aqueous environments (Ghobadian et al 2015). Studies on inorganic NP have shown that, even at sublethal concentrations, these NPs can cause alterations in the hatching rate, malformations, survival and production of reactive oxygen species (Ahmad et al 2015;Ašmonaitė et al 2016;Fang et al 2015;Ghobadian et al 2015;Hu et al 2017;Wehmas et al 2015;Zhao et al 2013). Fent et al (2010) managed to achieve a fluorescent silica nanoparticle nontoxic for zebrafish embryos.…”

Section: Chen Et Al 2011mentioning

confidence: 99%

Relation between biophysical properties of nanostructures and their toxicity on zebrafish

et al. 2017

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In recent years, the use of commercial nanoparticles in different industry and health fields has increased exponentially. However, the uncontrolled application of nanoparticles might present a potential risk to the environment and health. Toxicity of these nanoparticles is usually evaluated by a fast screening assay in zebrafish (Danio rerio). The use of this vertebrate animal model has grown due to its small size, great adaptability, high fertilization rate and fast external development of transparent embryos. In this review, we describe the toxicity of different micro-and nanoparticles (carbon nanotubes, dendrimers, emulsions, liposomes, metal nanoparticles, and solid lipid nanoparticles) associated to their biophysical properties using this model. The main biophysical properties studied are size, charge and surface potential due to their impact on the environment and health effects. The review also discusses the correlation of the effects of the different nanoparticles on zebrafish. Special focus is made on morphological abnormalities, altered development and abnormal behavior. The last part of the review debates changes that should be made in future directions in order to improve the use of the zebrafish model to assess nanotoxicity.

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“…The swimming behaviour (locomotion) of juvenile zebrafish was determined by using the View Point ® Video tracking system (VideoTrack V3, ViewPoint Life Science Inc, Montreal, CAN). A customised protocol, composed of 10 sequentially interchanging dark-light cycles, was used to artificially stress fish and evoke visual-motor responses, as described by Ašmonaitė et al (2016). The behavioural experiments started with 30 min light acclimatisation, followed by interchanging cycles of 10 min darkness and then followed by 5 minutes of light stimulation (with 100% light saturation) (Ašmonaitė et al 2016).…”

Section: Behavioural Toxicity Assessmentmentioning

confidence: 99%

“…The uptake of and translocation to the brain through the olfactory tract have been proposed in this regard (Handy et al 2008;Shaw and Handy, 2011), and there is evidence that engineered metal NPs can enter brain tissues and act as neurotoxicants, subsequently causing sensory-motoric dysfunction. It has also been shown that silver nanoparticles cause behavioural alterations in developing zebrafish larvae (Powers et al 2011;Ašmonaitė et al 2016), whereas exposure to Au NPs in the early life stages of zebrafish result in abnormal swimming behaviour, persisting into adulthood (Truong et al 2012). …”

Section: Introductionmentioning

confidence: 99%