Beneficial effects of enhanced aeration using perfluorodecalin in Yarrowia lipolytica cultures for lipase production

Amaral, Priscilla Filomena Fonseca; Almeida, Ana Paula R. de; Peixoto, Tathiana; Rocha-Leão, Maria Helena M.; Coutinho, João A. P.; Coelho, Maria Alice Zarur

doi:10.1007/s11274-006-9229-y

Cited by 23 publications

(18 citation statements)

References 22 publications

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“…Due to the high oxygen mass transfer rate, the cells had more oxygen in the medium giving higher growth rates, and less time is necessary to obtain maximum cell concentration. The improvement of specific cellular growth of Y. lipolytica by OTR enhancement trough other means than pressure was previously observed, as is the case of oxygen carrier use, like perfluorocarbons [19].…”

Section: Air Effects On Cell Growthsupporting

confidence: 61%

“…Y. lipolytica W29 adapts rapidly to hyperbaric conditions; thus, these conditions can be imposed to cultures of this strain as a way of preventing oxygen limitation to cell growth and as a mean of enzyme production improvement such as lipases and SOD. Air pressure can be seen as an important operating parameter such as medium composition [19] and strain selection [23] for lipase production optimisation.…”

Section: Discussionmentioning

confidence: 99%

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Yarrowia lipolytica Growth Under Increased Air Pressure: Influence on Enzyme Production

Lopes

Gomes

Мота

et al. 2008

Appl Biochem Biotechnol

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Improvement of microbial cell cultures oxygenation can be achieved by the increase of total air pressure, which increases oxygen solubility in the medium. In this work, a pressurized bioreactor was used for Yarrowia lipolytica batch cultivation under increased air pressure from 1 to 6 bar. Cell growth was strongly enhanced by the pressure rise. Fivefold and 3.4-fold increases in the biomass production and in specific growth rate, respectively, were observed under 6 bar. The increase of oxygen availability caused the induction of the antioxidant enzyme superoxide dismutase, which indicates that the defensive mechanisms of the cells against oxidative stress were effective and cells could cope with increased pressure. The pregrowth of Y. lipolytica under increased pressure conditions did not affect the lipase production ability of the cells. Moreover, the extracellular lipase activity increased 96% using a 5-bar air pressure instead of air at 1-bar pressure during the enzyme production phase. Thus, air pressure increase in bioreactors is an effective mean of cell mass and enzyme productivity enhancement in bioprocess based in Y. lipolytica cultures.

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Section: Air Effects On Cell Growthsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Yarrowia lipolytica Growth Under Increased Air Pressure: Influence on Enzyme Production

Lopes

Gomes

Мота

et al. 2008

Appl Biochem Biotechnol

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“…For instance, lipase production by Y. lipolytica IMUFRJ 50 682 was favoured at high oxygen availability, as demonstrated in experiments with perfluorocarbons. 38,39 Also, lipase activity in cultures of Y. lipolytica W29 (ATCC 20 460) was increased at improved oxygenation in pressurised bioreactors. 40 Once again, the diverse behaviour of different strains is stressed.…”

Section: Batch Enzyme Production In Bench-scale Bioreactorsmentioning

confidence: 99%

A comprehensive study of lipase production byYarrowia lipolyticaCECT 1240 (ATCC 18942): from shake flask to continuous bioreactor

Deive

Sanromán

Longo

2009

J of Chemical Tech & Biotech

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BACKGROUND: Lipases are commercially important enzymes, and the development and optimization of their production processes are of great interest. The diversity of behaviours between strains stresses the need for research on this topic, especially when bioreactor culture is considered. The study of a continuous operating mode is especially attractive, since very scarce information is available on its application to microbial lipases production.

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“…Preliminary shake-flask experiments showed that the use of perfluorodecalin as an oxygen carrier in the fermentation medium increased lipase production from Yarrowia lipolytica (Amaral et al, 2006a(Amaral et al, , 2007. Frost and Moss (1987) results indicate that improvements in aeration by agitation or air sparging are beneficial to lipase production by single cell organisms and filamentous moulds.…”

Section: Introductionmentioning

confidence: 99%

Optimization of oxygen mass transfer in a multiphase bioreactor with perfluorodecalin as a second liquid phase

Amaral

Freire

Rocha-Leão

et al. 2007

Biotech & Bioengineering

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Oxygenation is an important parameter involved in the design and operation of mixing-sparging bioreactors and it can be analyzed by means of the oxygen mass transfer coefficient (k(L)a). The operational conditions of a stirred, submerged aerated 2-L bioreactor have been optimized by studying the influence of a second liquid phase with higher oxygen affinity (perfluorodecalin or olive oil) in the k(L)a. Using k(L)a measurements, the influence of the following parameters on the oxygen transfer rate was evaluated: the volume of working medium, the type of impellers and their position, the organic phase concentration, the aqueous phase composition, and the concentration of inactive biomass. This study shows that the best experimental conditions were achieved with a perfluorodecalin volume fraction of 0.20, mixing using two Rushton turbines with six vertical blades and in the presence of YPD medium as the aqueous phase, with a k(L)a value of 64.6 h(-1). The addition of 20% of perfluorodecalin in these conditions provided a k(L)a enhancement of 25% when pure water was the aqueous phase and a 230% enhancement when YPD medium was used in comparison to their respective controls (no perfluorodecalin). Furthermore it is shown that the presence of olive oil as a second liquid phase is not beneficial to the oxygen transfer rate enhancement, leading to a decrease in the k(L)a values for all the concentrations studied. It was also observed that the magnitude of the enhancement of the k(L)a values by perfluorodecalin depends on the biomass concentration present.

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Beneficial effects of enhanced aeration using perfluorodecalin in Yarrowia lipolytica cultures for lipase production

Cited by 23 publications

References 22 publications

Yarrowia lipolytica Growth Under Increased Air Pressure: Influence on Enzyme Production

Yarrowia lipolytica Growth Under Increased Air Pressure: Influence on Enzyme Production

A comprehensive study of lipase production byYarrowia lipolyticaCECT 1240 (ATCC 18942): from shake flask to continuous bioreactor

Optimization of oxygen mass transfer in a multiphase bioreactor with perfluorodecalin as a second liquid phase

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