Beneficial Effects of Human Umbilical Cord Blood Mononuclear Cells on Persistent Erectile Dysfunction After Treatment of 5-Alpha Reductase Inhibitor in Rats

İntrakavernosal Si̇ldenafi̇li̇n Siçanlarda Dutasterid Tedavi̇si̇ Sonrasi Geli̇şen Erektil Di̇sfonksi̇yon Üzeri̇ne Yararli Etki̇si̇

“…Pinsky et al [31] displayed no differences in SNP-induced relaxant responses between control and 4week dutasteride treated rats. However, another study showed that relaxation responses to SNP were decreased after 12-week dutasteride treatment [12]. This difference was most probably due to the duration of dutasteride treatment.…”

“…In addition, the relaxation response to ACh was lowered in the 8-week dutasteride and 2-week washout groups [19]. Furthermore, the endothelium-dependent relaxation responses were lower after both 8-week and 12-week dutasteride treatment [12]. According to the results, the reduction in the endothelium-dependent relaxation response is likely to be associated with a decreased in free and total T levels following 5-ARIs treatment [12].…”

“…Rats were randomly divided into three groups; control (n=10), 10 weeks of dutasteride treatment (n=10), and 6 weeks of dutasteride treatment followed by 4 weeks of washout (n=10). As in previous studies [11,12,19], dutasteride was administered in drinking water (0.5 mg/rat/day). Dutasteride treatment was performed during 6 and 8 weeks in a previous study [19].…”

“…[20], polyethylene-50 tubing was inserted into the right crura of the penis and the carotid artery to measure ICP and MAP with a transducer (Statham, Oxnard, CA, USA) and a data acquisition system (Biopac MP 100 System, Santa Barbara, CA, USA). Following the detection of cavernosal nerve (CN), the CN was stimulated (2.5, 5, and 7.5 V, 15 Hz, 1 ms pulse width) with a stainless-steel bipolar-hook stimulating electrode and a square pulse stimulator (Grass Instruments, Quincy, MA) [12]. After intracavernosal injection of sildenafil (0.3 mg/kg) [21][22][23], the measurements were repeated in dutasteride-treated animals.…”

“…Furthermore, in preclinical data, dutasteride treatment induced ED in rats, even after withdrawal of dutasteride [11,12]. In addition, PDE5Is are the preferred choice, even though there are conflicting results [13][14][15].…”