Beneficial use of lignosulfonates in in vitro plant cultures: stimulation of growth, of multiplication and of rooting

Abstract

Lignosulfonates (LIGNs), low-cost by-products from the paper industry, are already commercialized as fertilizers; they stimulate both vegetative and reproductive growths and fructification. LIGNs have been tested in in vitro cultures and here too, they improve shoot growth and vigor, and rooting of various plant materials. This study aimed at to extend the in vitro application of LIGNs at different developmental stages in order to increase the productivity of systems generating vitroplants. The present results showed the beneficial effects of various LIGN applications on growth of a tropical orchid, Phalaenopsis, multiplication of Saintpaulia ionantha and rooting of poplar and Sequoiadendron sempervirens shoot cuttings. One of the most interesting observations was the stimulating effect of Ca-chelated LIGN on growth of Phalaenopsis and on rooting of Sequoiadendron. The significant and reproducible effects of LIGNs at several steps of micropropagation of different plant materials represent a potential tool to improve quality without embarrassing sideeffects.

Use of the stable isotope 57 Fe to track the efficacy of the foliar application of lignosulfonate/Fe3+ complexes to correct Fe deficiencies in cucumber plants

“…These groups could decrease the degradation of auxin by auxin oxidase via a competition mechanism (phenolics are also substrates for auxin oxidase). This protection of auxins could increase the formation of roots and indirectly the number of cytokinin synthesis sites, a possible explanation of further improvement of plant growth 38. Another explanation is the implication of quinones 39.…”

“…Another explanation is the implication of quinones 39. Docquier et al 38 suggest that promotion of cytokinin degradation by quinone componenets of the LS favor rooting. The quinone content of the LS tested was not determined, but their phenolic content was similar (Table 1).…”

Effects of Lignosulfonates on Callus Proliferation and Shoot Induction of Recalcitrant Indica Rice

“…Similarly, addition of CaLS was also reported to enhance callus growth of poplar (Kevers et al 1999), growth of Phalaenopsis and as well as rooting of Sequoiadendron (Docquier et al 2007). According to Docquier et al (2007), the effects of LS could be indirect or 'delayed' and they hypothesized that CaLS may regulate either hormonal changes, mineral balancing or regulates the numbers of auxin receptor and their affinity to free auxin. In general, micronutrient ions such as Fe, Zn and Mn are metal ions that could easily precipitate when there are changes in pH and chemical compositions.…”

Lignocellulosics as a Renewable Feedstock for Chemical Industry: Chemicals from Lignin

“…Slow-release fertilisers based on nitrogen-substituted lignins have been obtained by reacting ammonia with lignocellulosic biomass [120][121][122], soda lignin [123], kraft lignin [124], or technical grade lignins [125,126], and lignosulfonates have been employed in matrices for micronutrient fertilisers [127][128][129][130][131][132]. Partial hydrolysis of lignin with superheated steam has been applied to produce material suitable as a controlled-release coating for fertiliser [133][134][135], and lignin-based controlled-release formulations have also been prepared for herbicides and pesticides [136][137][138][139].…”