Benzopyrene induces oxidative stress and increases expression and activities of antioxidant enzymes, and CYP450 and GST metabolizing enzymes in Ulva lactuca (Chlorophyta)

González, Alberto; Espinoza, Daniel; Vidal, Constanza; Moenne, Alejandra

doi:10.1007/s00425-020-03508-w

Cited by 15 publications

(12 citation statements)

References 25 publications

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“…B[ a ]P also decreases the level of reduced glutathione (GSH), vitamin C, and vitamin E, as well as increases the level of lipid peroxidation and protein carbonyl formation, as well as increases the expression of CYP1A1 and proinflammatory cytokines. Metabolites of B[ a ]P (mainly BPDE) and ROS created during transformation of B[ a ]P are the most important contributors to the oxidative effects provoked by this substance [ 102 , 103 , 104 , 105 , 106 ] ( Figure 5 ).…”

Section: Metabolism Of B[ a ]Pmentioning

confidence: 99%

“…Moreover, B[ a ]P has been reported to increase gene expression associated with inflammation and oxidative stress, as well as to deplete the expression and the activities of antioxidative enzymes, i.e., CAT, GPx, SOD, GST, and GR. Furthermore, B[ a ]P depleted nonenzymatic antioxidants levels, including GSH, Vit C, and Vit E. Metabolites of B[ a ]P (mainly BPDE) have been shown to induce ROS formation, as well as cause lipid peroxidation and protein carbonylation ( Figure 6 ) [ 102 , 103 , 104 , 105 ].…”

Section: Metabolism Of B[ a ]Pmentioning

confidence: 99%

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Benzo[a]pyrene—Environmental Occurrence, Human Exposure, and Mechanisms of Toxicity

Bukowska

Mokra

Michałowicz

2022

IJMS

183

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Benzo[a]pyrene (B[a]P) is the main representative of polycyclic aromatic hydrocarbons (PAHs), and has been repeatedly found in the air, surface water, soil, and sediments. It is present in cigarette smoke as well as in food products, especially when smoked and grilled. Human exposure to B[a]P is therefore common. Research shows growing evidence concerning toxic effects induced by this substance. This xenobiotic is metabolized by cytochrome P450 (CYP P450) to carcinogenic metabolite: 7β,8α-dihydroxy-9α,10α-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE), which creates DNA adducts, causing mutations and malignant transformations. Moreover, B[a]P is epigenotoxic, neurotoxic, and teratogenic, and exhibits pro-oxidative potential and causes impairment of animals’ fertility. CYP P450 is strongly involved in B[a]P metabolism, and it is simultaneously expressed as a result of the association of B[a]P with aromatic hydrocarbon receptor (AhR), playing an essential role in the cancerogenic potential of various xenobiotics. In turn, polymorphism of CYP P450 genes determines the sensitivity of the organism to B[a]P. It was also observed that B[a]P facilitates the multiplication of viruses, which may be an additional problem with the widespread COVID-19 pandemic. Based on publications mainly from 2017 to 2022, this paper presents the occurrence of B[a]P in various environmental compartments and human surroundings, shows the exposure of humans to this substance, and describes the mechanisms of its toxicity.

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Section: Metabolism Of B[ a ]Pmentioning

confidence: 99%

Section: Metabolism Of B[ a ]Pmentioning

confidence: 99%

Benzo[a]pyrene—Environmental Occurrence, Human Exposure, and Mechanisms of Toxicity

Bukowska

Mokra

Michałowicz

2022

IJMS

183

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“…González et al [33] showed that BaP was rapidly incorporated and metabolized in green algae Ulva lactuca, and induced oxidative stress and activation of antioxidant enzymes, as well as CYP450 and GST metabolizing enzymes in tested organism. BaP induced oxidative stress by changes in levels of hydrogen peroxide, superoxide anions, and lipoperoxides, as well as changes in activities of antioxidant enzymes: SOD, CAT, AP, GR, and GPx.…”

Section: The Effect Of Bap On Oxidative Stress In Vitro and In Vivomentioning

confidence: 99%

“…Numerous studies have indicated oxidative changes caused by BaP both in vitro [30,[34][35][36] and in vivo [31][32][33]37] (Table 1). Release of IL-1β and TNF-α from these cells [30] 1; 5 µM A549 and MCF-7 cancer cells Formation of ROS by influencing ROS/HIF-1α/HO-1 signaling pathway [34] 5 µM Human lung epithelial cells (BEAS-2B) ROS production and DNA damage [35] 100 nM; 1 µM Human monocytes and U937 cells ROS production, increase in the expression of CYP1A1 gene, and induction of NF-κB pathway [36] 250 mg kg -1 Mice-maternal exposure to BaP Increased expression of CYP1A1, SOD1, and SOD2 gene, and repressed GPX1 gene [37] 10; 20 µM Zebrafish embryos Decrease of CAT and SOD1 gene expression, increase H 2 O 2 and MDA level [31] 0.2 mg L −1 Adult zebrafish…”

Section: The Effect Of Bap On Oxidative Stress In Vitro and In Vivomentioning

confidence: 99%

Molecular Mechanisms of Action of Selected Substances Involved in the Reduction of Benzo[a]pyrene-Induced Oxidative Stress

Bukowska

Duchnowicz

2022

Molecules

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Benzo[a]pyrene (BaP) is a polycyclic aromatic hydrocarbon (PAH) primarily formed by burning of fossil fuels, wood and other organic materials. BaP as group I carcinogen shows mutagenic and carcinogenic effects. One of the important mechanisms of action of (BaP) is its free radical activity, the effect of which is the induction of oxidative stress in cells. BaP induces oxidative stress through the production of reactive oxygen species (ROS), disturbances of the activity of antioxidant enzymes, and the reduction of the level of non-enzymatic antioxidants as well as of cytokine production. Chemical compounds, such as vitamin E, curcumin, quercetin, catechin, cyanidin, kuromanin, berberine, resveratrol, baicalein, myricetin, catechin hydrate, hesperetin, rhaponticin, as well as taurine, atorvastatin, diallyl sulfide, and those contained in green and white tea, lower the oxidative stress induced by BaP. They regulate the expression of genes involved in oxidative stress and inflammation, and therefore can reduce the level of ROS. These substances remove ROS and reduce the level of lipid and protein peroxidation, reduce formation of adducts with DNA, increase the level of enzymatic and non-enzymatic antioxidants and reduce the level of pro-inflammatory cytokines. BaP can undergo chemical modification in the living cells, which results in more reactive metabolites formation. Some of protective substances have the ability to reduce BaP metabolism, and in particular reduce the induction of cytochrome (CYP P450), which reduces the formation of oxidative metabolites, and therefore decreases ROS production. The aim of this review is to discuss the oxidative properties of BaP, and describe protective activities of selected chemicals against BaP activity based on of the latest publications.

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“…The oxidative stress triggered by BaP induced the increase of ROS production in vitro [15]. As shown in Figure 2D, compared to the control group, BaP-exposure increased the intracellular ROS level by 970.7% (p < 0.01), which indicated that BaP-exposure caused fatal oxidative stress in Caco-2 cells.…”

Section: Effect Of Cpd-2 On Bap-induced Oxidative Stress and In Ammation In Caco-2 Cellsmentioning

confidence: 82%

Citrus Peels Phenolic Derivatives Alleviate Benzo[α]pyrene-Induced Inflammatory Injury of Human Intestinal Epithelial Cells Through NLRP3 and AhR Signaling Pathways

Liang

Wang

Luo

et al. 2021

Preprint

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Benzo[α]pyrene (BaP) is ubiquitous in foods, and possesses a fatal cytotoxicity. In current study, ten Citrus peels (Chenpi) phenolic derivatives (CPDs) were isolated in a cell model of human intestinal epithelial (Caco-2) cells under BaP-exposure by a bio-assay guided method. Among them, methyl (3,4,5-trimethoxybenzoyl) valylphenylalaninate (Citrus peels phenolic derivative-2, CPD-2) performed the most protective activity by promoting the antiinflammatory potential on BaP-induced Caco-2 cells. CPD-2 inhibited BaP-induced intracellular ROS over-production and inflammatory epithelial cytokine, IL-4, IL-8, TNF-α, IL-1β and IL-18 over-expression, but not IL-6. CPD-2 also inhibited BaP-induced NLRP3 inflammasome and AhR signaling pathway activation. Overall, CPD-2 attenuates BaP-induced apoptotic death via promoting the antiinflammatory potentials by inhibiting the NLRP3 and AhR signaling pathways activation of Caco-2 cells. Finally, the Citrus peels phenolic derivatives was observed for the first time against BaP-induced inflammation and oxidative stress in human intestinal epithelial cells.

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Benzopyrene induces oxidative stress and increases expression and activities of antioxidant enzymes, and CYP450 and GST metabolizing enzymes in Ulva lactuca (Chlorophyta)

Abstract: Main conclusionBenzopyrene is rapidly incorporated and metabolized, and induces oxidative stress and activation of antioxidant enzymes, and CYP450 and GST metabolizing enzymes in Ulva lactuca.

Cited by 15 publications

References 25 publications

Benzo[a]pyrene—Environmental Occurrence, Human Exposure, and Mechanisms of Toxicity

Benzo[a]pyrene—Environmental Occurrence, Human Exposure, and Mechanisms of Toxicity

Molecular Mechanisms of Action of Selected Substances Involved in the Reduction of Benzo[a]pyrene-Induced Oxidative Stress

Citrus Peels Phenolic Derivatives Alleviate Benzo[α]pyrene-Induced Inflammatory Injury of Human Intestinal Epithelial Cells Through NLRP3 and AhR Signaling Pathways

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