Best laboratory practices and therapeutic interventions to reduce sperm DNA damage

Martínez, Marlon; Majzoub, Ahmad

doi:10.1111/and.13736

Cited by 11 publications

(8 citation statements)

References 193 publications

(214 reference statements)

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“…Our results suggest that high level of DNA fragmentation may not be accompanied by oxidized macromolecules level elevation and additional factors such as, for example, coronavirus infection, can be required to alter the pro- and antioxidant systems balance in semen. Therefore, the absence of oxidative stress verification in men and monitoring of the ROS level during therapy is a potential problem [ 54 ], since there is a risk of reductive stress induction due to excessive therapy with antioxidants [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress Markers and Sperm DNA Fragmentation in Men Recovered from COVID-19

Shcherbitskaia

Komarova

Milyutina

et al. 2022

IJMS

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SARS-CoV-2 negatively affects semen characteristics, impairs various biochemical processes in seminal fluid and within spermatogenic cells ultimately leading to male fertility decline. However, the distinct mechanisms, in particular, the role of oxidative stress on the consequences of coronavirus infection, have not been well investigated, which is the purpose of the present study. The standard semen parameters, its pro- and antioxidant system state, as well as the level of sperm DNA fragmentation, were assessed in 17 semen samples of men five months after the coronavirus infection and in 22 age-matched control patients. We determined that the DNA fragmentation rate negatively correlated with the period after coronavirus recovery, as well as seminal fluid superoxide dismutase activity and uric acid level. It was demonstrated that COVID-19 is not always associated with increased DNA fragmentation, allowing them to be considered as two independent factors. Thus, the most significant changes were noted in the samples of men after COVID-19 and abnormal TUNEL results: increased round cell number, decreased seminal fluid’s nitrotyrosine level, and total antioxidant capacity and Zn, as well as an increased 8-hydroxy-2′-deoxyguanosine level within spermatozoa. The data obtained indicate that increased DNA fragmentation and diminished semen quality in men can be the result of an imbalance in semen pro- and antioxidant components after COVID-19.

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Section: Discussionmentioning

confidence: 99%

Oxidative Stress Markers and Sperm DNA Fragmentation in Men Recovered from COVID-19

Shcherbitskaia

Komarova

Milyutina

et al. 2022

IJMS

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“…Overall, several studies that explore the effects of antioxidants therapy on SDF have been published ( 116 , 117 ). Various antioxidant regimens, whether single agents or combination therapy, were supplied to the populations of infertile men by the different studies and for different treatment durations, mostly ranging from two to six months.…”

Section: Management Of Elevated Sdfmentioning

confidence: 99%

Sperm DNA integrity and male infertility: a narrative review and guide for the reproductive physicians

Farkouh¹,

Salvio²,

Kuroda³

et al. 2022

Transl Androl Urol

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Background and Objective: Conventional semen analysis (SA) remains an essential tool in the initial male fertility evaluation and subsequent follow-up. However, it neither provides information about the functional status of spermatozoa nor addresses disorders such as idiopathic or unexplained infertility (UI).Recently, assessment of sperm DNA fragmentation (SDF) has been proposed as an extended sperm test that may help overcome these inherent limitations of basic SA. In this review, we aim to: (I) discuss the pathophysiological aspects of SDF, including natural repair mechanisms, causes, and impact on reproductive outcomes; (II) explain different assessment tools of SDF, and describe potential therapeutic options to manage infertile men with high SDF; and (III) analyse the strengths, weaknesses, opportunities and threats (SWOT) of current research on the topic.Methods: This review was constructed from original studies, systematic reviews and meta-analyses that were published over the years up until August 2021, related to the various aspects of SDF.

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“…However, these effects on sperm function cannot be detected using routine semen analysis. Martinez and Majzoub (2020) reviewed the common techniques available to evaluate SDF and discussed the clinical indications for its testing, such as in cases of unexplained infertility, clinical varicocele, recurrent pregnancy loss, and poor ART outcomes (Martinez & Majzoub, 2020). The authors also reviewed the feasibility of antioxidant usage to counter sperm DNA damage in these patient cohorts as well as during sperm selection and in an ART setting.…”

mentioning

confidence: 99%

Afterword: An update on clinical utility and diagnostic value of various andrological techniques

et al. 2021

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Best laboratory practices and therapeutic interventions to reduce sperm DNA damage

Cited by 11 publications

References 193 publications

Oxidative Stress Markers and Sperm DNA Fragmentation in Men Recovered from COVID-19

Oxidative Stress Markers and Sperm DNA Fragmentation in Men Recovered from COVID-19

Sperm DNA integrity and male infertility: a narrative review and guide for the reproductive physicians

Afterword: An update on clinical utility and diagnostic value of various andrological techniques

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