Bilayer membrane interactions with nanofabricated scaffolds

Collier, C. Patrick

doi:10.1016/j.chemphyslip.2015.07.013

Cited by 6 publications

(3 citation statements)

References 142 publications

(136 reference statements)

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“…These systems are convenient for varying the type of antigen and also introducing spatial and geometric constraints. Supported lipid bilayers (SLBs) have gained traction in their utility to study the IS, as they allow for maintenance of the type of fluidity that would be encountered in cell membranes ( Figure 5C) (85,114,(129)(130)(131)(132)(133)(134). There are many different ways in which to assemble SLBs, which have historically also found high utility for studying the electrophysiology of ion channels, pumps and transporters (131,135,136).…”

Section: Experimental Setup For Imaging the Ismentioning

confidence: 99%

Considerations of Antibody Geometric Constraints on NK Cell Antibody Dependent Cellular Cytotoxicity

Murin

2020

Front. Immunol.

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It has been well-established that antibody isotype, glycosylation, and epitope all play roles in the process of antibody dependent cellular cytotoxicity (ADCC). For natural killer (NK) cells, these phenotypes are linked to cellular activation through interaction with the IgG receptor FcγRIIIa, a single pass transmembrane receptor that participates in cytoplasmic signaling complexes. Therefore, it has been hypothesized that there may be underlying spatial and geometric principles that guide proper assembly of an activation complex within the NK cell immune synapse. Further, synergy of antibody phenotypic properties as well as allosteric changes upon antigen binding may also play an as-of-yet unknown role in ADCC. Understanding these facets, however, remains hampered by difficulties associated with studying immune synapse dynamics using classical approaches. In this review, I will discuss relevant NK cell biology related to ADCC, including the structural biology of Fc gamma receptors, and how the dynamics of the NK cell immune synapse are being studied using innovative microscopy techniques. I will provide examples from the literature demonstrating the effects of spatial and geometric constraints on the T cell receptor complex and how this relates to intracellular signaling and the molecular nature of lymphocyte activation complexes, including those of NK cells. Finally, I will examine how the integration of high-throughput and "omics" technologies will influence basic NK cell biology research moving forward. Overall, the goal of this review is to lay a basis for understanding the development of drugs and therapeutic antibodies aimed at augmenting appropriate NK cell ADCC activity in patients being treated for a wide range of illnesses.

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Section: Experimental Setup For Imaging the Ismentioning

confidence: 99%

Considerations of Antibody Geometric Constraints on NK Cell Antibody Dependent Cellular Cytotoxicity

Murin

2020

Front. Immunol.

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“…The formation of lamellar lipid bilayers on planar supports have significantly advanced our understanding of the physicochemical properties of membranes and their role in modulating molecular interactions and function in a membrane environment. However, native membranes also exhibit diverse nanoscale structures and topologies, and characterizing membranes of different nanostructures has been greatly facilitated by advances in nanofabrication technology which configure the lipid vesicles or lamellar lipid bilayers on a substrate of different nanostructured scaffolds. ,− These model membranes formed on various nanofabricated substrates as exemplified in Figure enable the role of different underlying membrane morphologies that modulate the physical properties and lipid organization to be characterized and allow the molecular basis of membrane structural organization on molecule interactions. Integration of micro- and nanostructures as sensing platforms with nanoplasmonic and zero-mode waveguides then enhance both the sensitivity and lateral resolution of dynamic changes in membrane structures.…”

Section: Model Membrane Systemsmentioning

confidence: 99%

Exploring Molecular-Biomembrane Interactions with Surface Plasmon Resonance and Dual Polarization Interferometry Technology: Expanding the Spotlight onto Biomembrane Structure

et al. 2018

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The molecular analysis of biomolecular-membrane interactions is central to understanding most cellular systems but has emerged as a complex technical challenge given the complexities of membrane structure and composition across all living cells. We present a review of the application of surface plasmon resonance and dual polarization interferometry-based biosensors to the study of biomembrane-based systems using both planar mono- or bilayers or liposomes. We first describe the optical principals and instrumentation of surface plasmon resonance, including both linear and extraordinary transmission modes and dual polarization interferometry. We then describe the wide range of model membrane systems that have been developed for deposition on the chips surfaces that include planar, polymer cushioned, tethered bilayers, and liposomes. This is followed by a description of the different chemical immobilization or physisorption techniques. The application of this broad range of engineered membrane surfaces to biomolecular-membrane interactions is then overviewed and how the information obtained using these techniques enhance our molecular understanding of membrane-mediated peptide and protein function. We first discuss experiments where SPR alone has been used to characterize membrane binding and describe how these studies yielded novel insight into the molecular events associated with membrane interactions and how they provided a significant impetus to more recent studies that focus on coincident membrane structure changes during binding of peptides and proteins. We then discuss the emerging limitations of not monitoring the effects on membrane structure and how SPR data can be combined with DPI to provide significant new information on how a membrane responds to the binding of peptides and proteins.

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“…Adsorbed liposomes onto structured surfaces can form fluid, supported lipid bilayers as realistic models for biomembranes capable of mimicking cellular functions such as transport and signaling . Micro‐ and nanopatterned surfaces can be utilized to mimic the geometries and scales of these environments, and to elucidate how biological phenomena that rely on diffusive transport are impacted in these spatially restricted environments …”

Section: Introductionmentioning

confidence: 99%

Geometry‐Dependent Nonequilibrium Steady‐State Diffusion and Adsorption of Lipid Vesicles in Micropillar Arrays

Liu

Abel

Collins

et al. 2019

Adv Materials Inter

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Micro‐ and nanofabricated sample environments are useful tools for characterizing diffusion in confined aqueous environments. The steady‐state diffusion and adsorption of unilamellar lipid vesicles in arrays of hydrophilic micropillars is investigated. Gradients in the coverage of fluorescently labeled, pillar‐supported lipid films, formed from vesicle fusion, are determined from 3D z‐stack images using confocal microscopy. The gradients are the result of preferential adsorption of vesicles near the tops of the pillars, which progressively deplete them from solution as they diffuse toward the base of the array. However, the increased propensity for vesicle adsorption near the pillar tops compared to the confined spaces between pillars results in the formation of confluent supported lipid bilayers at the pillar tops that resist the adsorption of additional vesicles while leaving the pillar surfaces below available for binding. This results in a reduction in the numbers of depleted vesicles compared to what one would anticipate based on diffusive fluxes. The resulting inhomogeneous spatial profiles of lipid structures on the pillars are the result of the system being maintained in a dissipative, nonequilibrium steady state during incubation of the pillar arrays in the vesicle solution, which is ultimately quenched by rinsing away the unbound, freely diffusing vesicles.

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Bilayer membrane interactions with nanofabricated scaffolds

Cited by 6 publications

References 142 publications

Considerations of Antibody Geometric Constraints on NK Cell Antibody Dependent Cellular Cytotoxicity

Considerations of Antibody Geometric Constraints on NK Cell Antibody Dependent Cellular Cytotoxicity

Exploring Molecular-Biomembrane Interactions with Surface Plasmon Resonance and Dual Polarization Interferometry Technology: Expanding the Spotlight onto Biomembrane Structure

Geometry‐Dependent Nonequilibrium Steady‐State Diffusion and Adsorption of Lipid Vesicles in Micropillar Arrays

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