Bile acids and conjugates identified in metabolic disorders by fast atom bombardment and tandem mass spectrometry

Abstract

From a study of the collision-activated fragmentation of bile acids, a qualitative analytical method based on negative ion fast atom bombardment tandem mass spectrometry has been developed. The times for sample preparation and analyses are short. Both free and conjugated bile acids are detected as they occur in biological fluids, without derivatization. For identifying bile acids and conjugates, the method offers better specificity and sensitivity than does the fast atom bombardment mass spectrometric technique alone. Specific scan modes have been developed for the selective detection of taurine conjugates, Δ4-unsaturated taurine conjugates, Δ4-3-keto free acids and their glycine conjugates, free acids and glycine conjugates bearing a hydroxyl group at the C-12 position, sulfates of glycine and taurine conjugates, and a C29 dicarboxylic bile acid, specific for generalized peroxisomal disorders. Applications of this technique demonstrate its potential usefulness, principally in the diagnosis of several peroxisomal disorders.

Effect of proglycosyn and other phenolic compounds on glycogen metabolism in isolated hepatocytes

“…The tandem mass spectrometry collision gas was xenon at 100 mPa. For details concerning the methodology, see Libert et al (1991).…”

Single‐Step analysis of individual conjugated bile acids in human bile using 1H NMR spectroscopy

“…In view of the multitude of useful as well as deleterious roles played by the bile acids, measurement of their conjugation pattern is necessary for understanding the pathophysiology of these diseases. Several methods for bile acids analysis have been proposed (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25). Of these, liquid chromatography-mass spectrometry (22)(23)(24)(25) and liquid chromatography-electrospray tandem mass spectrometry (2) methods are reported to be useful for routine qualitative/quantitative analysis.…”

Severe Cholestasis Induced by Cholic Acid Feeding in Knockout Mice of Sister of P–Glycoprotein

“…Bile Acid Extraction and Analysis. Extraction 20 and quantitation of bile acids by liquid chromatography-mass spectrometry (LC/MS/MS) 12,21 were performed using methods as previously reported. Taurocholate in some bile samples was quantified by HPLC using the technique established by Rossi et al 22 and modified by Hagey et al 23 HPLC analysis was carried out with a Waters 600 pump and controller and a 486 UV detector.…”