Binary System of Grading Epithelial Dysplasia in Oral Leukoplakias

Carmo, María Auxiliadora Vieira do; Caldeira, Patrícia Carlos

doi:10.5772/54466

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…For the present study, although the samples were reported according to the 3-tier system (2017 WHO guidelines [31]) by pathologists at St James's Hospital, they were regrouped according to the 2-tier system [32][33][34][35][36] of low-grade and highgrade dysplasia.…”

Section: Analysis Of High and Low Grade Dysplasia For Pre-cancer Pati...mentioning

confidence: 99%

Identification of precancer oral lesions using minimally invasive sampling techniques and Raman microspectroscopy

Behl¹,

Calado²,

Flint³

et al. 2023

Optical Biopsy XXI: Toward Real-Time Spectroscopic Imaging and Diagnosis

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Background and aim:The gold standard diagnosis of oral pre-cancer/cancer is an invasive biopsy followed by a histological examination, which may present psychological trauma and risk of infection to patients. Moreover, multiple biopsies are often required to monitor precancer lesions' progression. With the aim of reducing the need for multiple biopsies, this study was undertaken to identify pre-cancerous lesions using minimally invasive brush biopsy and saliva samples, analysed by Raman spectroscopy. Method:In this study, brush biopsies and saliva samples were collected from healthy donors (n = 50) and from patients with dysplastic lesions (n = 50). Raman spectra were acquired from the nucleus and cytoplasm of each cell and from liquid saliva. Results:A discriminant analysis of brush biopsy samples indicated that dysplastic cells could be successfully differentiated from normal cells with 96% accuracy based on changes in lipid content, while a similar analysis of saliva samples achieved 86% accuracy, based on protein spectral changes. In addition, discrimination between high-grade and low-grade dysplasia was achieved with 80% accuracy using cell samples, and discrimination between dysplasia and oral squamous cell carcinoma was achieved with 75% accuracy using saliva samples. Conclusions:This study successfully demonstrated that Raman spectroscopy and minimally invasive brush biopsy and saliva samples could be used for the early detection of precancerous lesions and could have a role in monitoring patients with dysplasia, reducing the need for multiple biopsies.

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Section: Analysis Of High and Low Grade Dysplasia For Pre-cancer Pati...mentioning

confidence: 99%

Identification of precancer oral lesions using minimally invasive sampling techniques and Raman microspectroscopy

Behl¹,

Calado²,

Flint³

et al. 2023

Optical Biopsy XXI: Toward Real-Time Spectroscopic Imaging and Diagnosis

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“…Lesi pada penelitian Cavalcante et al 7 dikategorikan sebagai displasia sedang, sedangkan lesi pada penelitian ini berdasarkan klasifikasi WHO termasuk dalam kategori displasia ringan. 19 Lesi pada penelitian ini dan pada penelitian Cavalcante et al 7 menunjukkan kemiripan, hanya saja berbeda pada derajat progresivitasnya. Perbedaan tersebut dapat terjadi karena perbedaan durasi induksi karsinogenesis.…”

Section: Hasil Penelitianunclassified

Efektivitas Ekstrak Etanol Daun Sirsak (Annona muricata Linn) dalam Mencegah Displasia Dorsum Lingue Tikus Galur Sprague Dawley yang Diinduksi 7,12 Dimetilbenz(Α)Antrasena

Prihatiningsih

Haniastuti

Agustina

2021

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Polycyclic aromatic hydrocarbons (PAH) is one of the largest group of carcinogen in the environment. An agent that has antigenotoxic and anticarcinogen potency is needed to prevent DNA damage and carcinogenesis. Soursop leaves have a chemopreventive action. This study was aimed to assess the effectiveness of ethanolic extract of soursop leaves in prevention of dysplasia of upper surface of tongue in rats. This was a true laboratory experimental study with the post test-only control group design, using 24 male Sprague Dawley rats divided into six groups. The upper surfaces of tongues of group I-III were induced by DMBA topically three times a week for 16 weeks; group II and III were induced by DMBA added soursop leaves ethanolic extract of 100 and 200 mg/kg body weight daily for 18 weeks; group IV was given soursop leaves ethanolic extract 200 mg/kg body weight; group V was given DMSO 1%; and group VI was untreated. After 18th week, rats were terminated and tongue necropsies in longitudinal anteroposterior direction were conducted. The clinical changes of the upper surfaces of the tongues were observed and the histological changes were observed by using HE staining to confirm signs of dysplasia. The Kruskal Wallis test showed differences between groups, and the Mann-Withney test showed not significantly decrease of mild dysplasia of group II and group III compared to group I (p>0.05). In conclusion, ethanolic extract of soursop leaves was not effective in prevention of dysplasia of the upper surface of rat’s tongue.Keywords: dysplasia; soursop leave extract; dorsal part of tongue; DMBA Abstrak: Senyawa golongan PAH merupakan salah satu kelompok karsinogen terbesar di lingkungan. Sebagai upaya untuk mencegah kerusakan DNA serta karsinogenesis, diperlukan suatu agen yang berpotensi antigenotoksik sekaligus antikarsinogenik. Daun sirsak terbukti mempunyai aksi kemopreventif. Penelitian ini bertujuan untuk mengkaji efektivitas ekstrak etanol daun sirsak dalam mencegah displasia pada dorsum lingue tikus. Jenis penelitian ialah true experimental laboratory dengan the post test-only control group design yang menggunakan 24 ekor tikus galur Sprague Dawley jantan, dibagi dalam enam kelompok. Dorsum lingue tikus kelompok I - III diinduksi DMBA secara topikal tiga kali seminggu selama 16 minggu; kelompok II dan III selain diinduksi DMBA, juga diberi ekstrak etanol daun sirsak 100 dan 200 mg/kg berat badan setiap hari selama 18 minggu; kelompok IV diberi ekstrak etanol 200 mg/kg BB; kelompok V diberi DMSO 1%; dan kelompok VI tidak diberi perlakuan. Terminasi dan nekropsi lidah tikus longitudinal anteroposterior dilaksanakan setelah minggu ke 18. Perubahan pada dorsum lingue diamati secara klinis dan perubahan histologik dilihat dengan pewarnaan HE untuk mengonfirmasi tanda displasia. Uji Kruskal Wallis menunjukkan perbedaan antar kelompok, sedangkan uji Mann Whitney menunjukkan penurunan tidak bermakna displasia ringan pada kelompok II dan III dibanding kelompok I. Simpulan penelitian ini ialah ekstrak etanol daun sirsak tidak efektif dalam mencegah displasia epitel dorsum lingue tikus.Kata kunci: displasia; ekstrak daun sirsak; dorsum lingue; DMBA

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Oral Potentially Malignant Disorders

2021

Handbook of Oral Pathology and Oral Medicine

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Binary System of Grading Epithelial Dysplasia in Oral Leukoplakias

Cited by 3 publications

References 35 publications

Identification of precancer oral lesions using minimally invasive sampling techniques and Raman microspectroscopy

Identification of precancer oral lesions using minimally invasive sampling techniques and Raman microspectroscopy

Efektivitas Ekstrak Etanol Daun Sirsak (Annona muricata Linn) dalam Mencegah Displasia Dorsum Lingue Tikus Galur Sprague Dawley yang Diinduksi 7,12 Dimetilbenz(Α)Antrasena

Oral Potentially Malignant Disorders

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