Binding induced isothermal amplification reaction to activate CRISPR/Cas12a for amplified electrochemiluminescence detection of rabies viral RNA via DNA nanotweezer structure switching

Nucleic acid isothermal amplification-based soft nanoarchitectonics as an emerging electrochemical biosensing platform

“…74 Ru(bpy) 3 2+ could be labeled on DNA sequence as signal probes (SP), which could perform satisfactory signal amplified ECL response. [75][76][77][78][79][80][81][82] For example, as shown in Fig. 3B, Yang's group constructed a tetrahedral DNA nanostructure on the electrode as capture probes and Ru(bpy) 3 2+ -labeled DNA sequence as signal probes.…”

A carbon dot-based nanoscale covalent organic framework as a new emitter combined with a CRISPR/Cas12a-mediated electrochemiluminescence biosensor for ultrasensitive detection of bisphenol A

“…32 Therefore, CRISPR/Cas12a-based biosensors have rapidly expanded from the field of fluorescence to the development of electrochemiluminescence sensing systems, which can detect nucleic acid and non-nucleic acid targets with high sensitivity. 33,34…”

Band-Edge Effect-Induced Electrochemiluminescence Signal Amplification Based on Inverse Opal Photonic Crystals for Ultrasensitive Detection of Carcinoembryonic Antigen

“…With regard to the determination of biomolecules, the primary research goal is to develop a high-efficiency sensing platform to magnify the ECL signals and thereby boost the sensitivity of ECL sensors. The conventional approaches employed in ECL assays for signal amplification could be classified into three categories: the first is developing novel luminophore species with high luminous efficiency − or accessory material with catalytic properties to accelerate the electrochemical process; the second is designing ingenious biological amplification strategies to increase the amount of target or probe sequence; and the third one is utilizing the functional substrates or signal indicators with specific signal regulation mechanisms . PCs, belonging to the third one, show significant potential in the ECL sensing platform due to their unique light manipulation characteristic.…”