Binding of phycoerythrin and its conjugates to murine low affinity receptors for immunoglobulin G

Takizawa, Fumiyoshi; Kinét, Jean Pierre; Adamczewski, Martin

doi:10.1016/0022-1759(93)90392-k

Cited by 14 publications

(7 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1). Although earlier reports indicated that PE can bind Fc receptors, these studies also showed that anti-Fc receptor antibodies and/or serum, or serum Ig completely block the interaction (Takizawa, et al, 1993; vanVugt, et al, 1996). Indeed, as Fc blockers, normal mouse serum and normal hamster serum were included in all our Fluorescence-activated Cell Sorting (FACS) analysis; we observed no above-background PE staining on myeloid cells or αβ T cells.…”

Section: Resultsmentioning

confidence: 97%

γδ T Cells Recognize a Microbial Encoded B Cell Antigen to Initiate a Rapid Antigen-Specific Interleukin-17 Response

et al. 2012

View full text Add to dashboard Cite

Summary γδ T cells contribute uniquely to host immune defense. However, how they function remains an enigma. Although it is unclear what most γδ T cells recognize, common dogma asserts that they recognize self-antigens. While they are the major initial Interleukin-17 (IL-17) producers in infections, it is unclear what is required to trigger these cells to act. Here, we report that a noted B cell antigen, the algae protein-phycoerythrin (PE) is an antigen for murine and human γδ T cells. PE also stained specific bovine γδ T cells. Employing this specificity, we demonstrated that antigen recognition, but not extensive clonal expansion, was required to activate naïve γδ T cells to make IL-17. In this activated state, γδ T cells gained the ability to respond to cytokine signals that perpetuated the IL-17 production. These results underscore the adaptability of lymphocyte antigen receptors and suggest a previously unrecognized antigen-driven rapid response in protective immunity prior to the maturation of classical adaptive immunity.

show abstract

Section: Resultsmentioning

confidence: 97%

γδ T Cells Recognize a Microbial Encoded B Cell Antigen to Initiate a Rapid Antigen-Specific Interleukin-17 Response

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Cells were incubated with 1 lg of primary antibody (anti-CD16/CD32 FcgIII/II receptor) for 10 min at room temperature to prevent nonspecific-antibody binding. 18 A rat anti-mouse CD45-APC antibody/a rat anti-CD34-FITC antibody or control antibody were added and incubated for 30 min at 4°C. Samples were analyzed by flow cytometry using a FACS Calibur instrument (Becton Dickinson, San Jose, CA) to quantify staining intensity.…”

Section: Total Circulating Endothelial Cells (Tcec) Analysismentioning

confidence: 99%

E7080, a novel inhibitor that targets multiple kinases, has potent antitumor activities against stem cell factor producing human small cell lung cancer H146, based on angiogenesis inhibition

Matsui¹,

Yamamoto²,

Funahashi³

et al. 2007

Intl Journal of Cancer

483

315

View full text Add to dashboard Cite

E7080 is an orally active inhibitor of multiple receptor tyrosine kinases including VEGF, FGF and SCF receptors. In this study, we show the inhibitory activity of E7080 against SCF-induced angiogenesis in vitro and tumor growth of SCF-producing human small cell lung carcinoma H146 cells in vivo. E7080 inhibits SCF-driven tube formation of HUVEC, which express SCF receptor, KIT at the IC 50 value of 5.2 nM and it was almost identical for VEGFdriven one (IC 50 5 5.1 nM). To assess the role of SCF/KIT signaling in tumor angiogenesis, we evaluated the effect of imatinib, a selective KIT kinase inhibitor, on tumor growth of H146 cells in nude mice. Imatinib did not show the potent antitumor activity in vitro (IC 50 5 2,200 nM), because H146 cells did not express KIT. However, oral administration of imatinib at 160 mg/kg clearly slowed tumor growth of H146 cells in nude mice, accompanied by decreased microvessel density. Oral administration of E7080 inhibited tumor growth of H146 cells at doses of 30 and 100 mg/kg in a dose-dependent manner and caused tumor regression at 100 mg/kg. While anti-VEGF antibody also slowed tumor growth, it did not cause tumor regression. These results indicate that KIT signaling has a role in tumor angiogenesis of SCF-producing H146 cells, and E7080 causes regression of H146 tumors as a result of antiangiogenic activity mediated by inhibition of both KIT and VEGF receptor signaling. E7080 may provide therapeutic benefits in the treatment of SCF-producing tumors. ' 2007 Wiley-Liss, Inc.

show abstract

“…Recently, another artifact in flow cytometric immunophenotyping assays has been shown to be mediated by Fc␥R, i.e., the binding of PE-Cy5 conjugates to human Fc␥RI (CD64) (25). These results, combined with the nonspecific binding of PE conjugates to murine Fc␥RII and Fc␥RIII that severily limits the use of PE in assays using murine cells (21), illustrate the importance of the Fc receptor system for flow cytometric immunophenotyping.…”

Section: Discussionmentioning

confidence: 88%

Analysis of factors contributing to the formation of mononuclear cell aggregates (?escapees?) in flow cytometric immunophenotyping

et al. 1997

View full text Add to dashboard Cite

Binding of phycoerythrin and its conjugates to murine low affinity receptors for immunoglobulin G

Cited by 14 publications

References 7 publications

γδ T Cells Recognize a Microbial Encoded B Cell Antigen to Initiate a Rapid Antigen-Specific Interleukin-17 Response

γδ T Cells Recognize a Microbial Encoded B Cell Antigen to Initiate a Rapid Antigen-Specific Interleukin-17 Response

E7080, a novel inhibitor that targets multiple kinases, has potent antitumor activities against stem cell factor producing human small cell lung cancer H146, based on angiogenesis inhibition

Analysis of factors contributing to the formation of mononuclear cell aggregates (?escapees?) in flow cytometric immunophenotyping

Contact Info

Product

Resources

About