Binding of Riboflavin to Lysozyme Promoted by Peroxidase‐generated Triplet Acetone

Durán, Nélson; Haun, Marcela; Toledo, Sonia M. de; Cilento, Giuseppe; Silva, Eduardo

doi:10.1111/j.1751-1097.1983.tb04467.x

Cited by 19 publications

(2 citation statements)

References 26 publications

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“…Riboflavin deficiency also decreased lysozyme activity in the intestine and gills of grass carp [ 12 , 13 ]. Riboflavin reacts with lysozyme under specific conditions, i.e., light-induced binding of riboflavin to lysozyme [ 62 ]. Such reactions might be induced by high riboflavin levels resulting in lower lysozyme activity as observed in R60 group in the present study.…”

Section: Discussionmentioning

confidence: 99%

Dietary Riboflavin Requirement of Pacific White Shrimp (Litopenaeus vannamei)

Sanjeewani

Lee

2023

Aquaculture Nutrition

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This study was conducted to determine the dietary riboflavin requirement and its effects on growth performance, feed utilization, innate immunity, and diet digestibility of Litopenaeus vannamei. A riboflavin-free basal diet (R0) was formulated as a control, and six other diets were prepared by adding riboflavin of 10, 20, 30, 40, 50, and 60 mg/kg to the basal diet (designated as R10, R20, R30, R40, R50, and R60, respectively). Quadruplicate groups of shrimp (initial average weight 0.17 ± 0.00 g ) were fed the diets six times a day for 8 weeks. Weight gain, specific growth rate, and protein efficiency ratio were significantly increased by riboflavin ( p < 0.05 ). The maximum values were observed in shrimp fed R40 diet. The highest activities of phenoloxidase, nitro blue tetrazolium, superoxide dismutase, and glutathione peroxidase were observed in shrimp fed R40 diet. Lysozyme activity was significantly higher in shrimp fed R30 and R40 diets than that of shrimp fed R60 diet ( p < 0.05 ). Intestinal villi were significantly longer in shrimp fed R50 and R60 diets compared to those of all other groups while the shortest villi were observed in R0 group ( p < 0.05 ). Intestinal villi were clearly distinguished in shrimp fed higher levels of riboflavin compared to those of shrimp fed R0 and R10 diets. Apparent digestibility coefficients of dry matter and protein in diets were not significantly affected by riboflavin levels ( p < 0.05 ). Whole-body proximate composition and hemolymph biochemical parameters were not significantly altered by dietary riboflavin ( p < 0.05 ). Therefore, the results of this study indicate that riboflavin is essential to enhance growth performance, feed utilization, nonspecific immunity, and intestine morphology of shrimp. An optimal riboflavin requirement for the maximum growth of L. vannamei seems to be approximately 40.9 mg/kg diet.

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Section: Discussionmentioning

confidence: 99%

Dietary Riboflavin Requirement of Pacific White Shrimp (Litopenaeus vannamei)

Sanjeewani

Lee

2023

Aquaculture Nutrition

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“…For the case of the peroxidase-catalyzed generation of triplet acetone, energy transfer to dibromoanthracene sulfonate ion [85], flavins [92,93], biacetyl [84], xauthene dyes [94], quinones [95], tyrosines [96], and chlorophyll-a [97][98][99] was observed. The latter process, promotion of photochemical reactions, was observed as phytochrome phototransformations [100], chlorpromazine photooxidation [101], photochemical-llke effect on DNA [102], tingle-strand breaks in DNA [103,104], damage in A-phage DNA [105,106], tryptophan photooxidation [107,108], conversion of colchicine into hmicolchicines [109], promotion of binding of riboflavin to lysozyme [110], photohemolysis in human red cells [111][112][113], and in leukocytes [114].…”

Section: Biological Generation Of Excited Statesmentioning

confidence: 99%

The role of singlet oxygen and triplet carbonyls in biological systems

Durán

Cadenas

1987

Reviews of Chemical Intermediates

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Viergliedrige cyclische Peroxide als Äquivalente angeregter Zustände: Eine neue Dimension der Bioorganischen Chemie

Adam

Cilento

1983

Angewandte Chemie

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Professor Gunther 0. Schenck zum 70. Geburtstag gewidmet Bei der Beschaftigung mit Chemilumineszenz-und Biolumineszenzvorgangen drangt sich unweigerlich der Gedanke an die viergliedrigen cyclischen Peroxide -1,2-Dioxetane und aPeroxylactone (1,2-Dioxetanone) -auf. Wahrend Chemilumineszenz und Biolumineszenz jedoch traditionelle Gebiete wissenschaftlicher Betiitigung sind, befabt man sich mit derartigen isolierbaren und charakterisierten hochenergetischen Molekiilen erst seit etwa einem Jahnehnt. Diese faszinierenden Verbindungen eaeugen in der Tat wahrend ihres thermischen Zerfalls elektronisch angeregte Produkte, die sich durch Lichtemission zu erkennen geben. Wichtiger ist noch, dab solche Umwandlungen bei enzymkatalysierten Lumineszenzund Dunkelprozessen beteiligt sind. In diesem Fortschrittsbericht sollen die neuesten Entwicklungen zusammengestellt werden. Dabei erheben wir keinen Anspruch auf Vollstandigkeit, sondern konzentrieren uns auf jene Aspekte, die weitere Aktivitaten erfordern. SyntheseDas erste stabile 1,2-Dioxetan, das Trimethylderivat 1, wurde 1969 durch die Kopecky-Reaktionsfolge [Reaktion

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Binding of Riboflavin to Lysozyme Promoted by Peroxidase‐generated Triplet Acetone

Abstract: When riboflavin and lysozyme are added to the 2-methylpropanallperoxidase/02 system, which generates triplet acetone, an adduct is formed to a small extent. The adduct can be separated by gel filtration and is similar to that prepared by irradiating riboflavin in the presence of lysozyme.

Cited by 19 publications

References 26 publications

Dietary Riboflavin Requirement of Pacific White Shrimp (Litopenaeus vannamei)

Dietary Riboflavin Requirement of Pacific White Shrimp (Litopenaeus vannamei)

The role of singlet oxygen and triplet carbonyls in biological systems

Viergliedrige cyclische Peroxide als Äquivalente angeregter Zustände: Eine neue Dimension der Bioorganischen Chemie

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