Binding of T lymphocytes to hippocampal neurons through ICAM-5 (telencephalin) and characterization of its interaction with the leukocyte integrin CD11a / CD18

Tian, Li; Kilgannon, Patrick; Yoshihara, Yoshihiro; Mori, Kensaku; Gallatin, W. Michael; Carpén, Olli; Gahmberg, Carl G.

doi:10.1002/1521-4141(200003)30:3<810::aid-immu810>3.0.co;2-x

Cited by 52 publications

(20 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The production of sICAM5 is known to occur during dendritic growth or glutamate activation (Tian et al, 2000, 2007). The mechanism by which sICAM5 escapes the extracellular fluid and crosses the blood brain barrier to enter the plasma has yet to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

The TARC/sICAM5 Ratio in Patient Plasma is a Candidate Biomarker for Drug Resistant Epilepsy

Pollard¹,

Eidelman²,

Mueller³

et al. 2013

Front. Neur.

View full text Add to dashboard Cite

Epilepsy is a common affliction that involves inflammatory processes. There are currently no definitive chemical diagnostic biomarkers in the blood, so diagnosis is based on a sometimes expensive synthesis of clinical observation, radiology, neuro-psychological testing, and interictal and ictal EEG studies. Soluble ICAM5 (sICAM5), also known as telencephalin, is an anti-inflammatory protein of strictly central nervous system tissue origin that is also found in blood. Here we have tested the hypothesis that plasma concentrations of select inflammatory cytokines, including sICAM5, might serve as biomarkers for epilepsy diagnosis. To test this hypothesis, we developed a highly sensitive and accurate electrochemiluminescent ELISA assay to measure sICAM5 levels, and measured levels of sICAM5 and 18 other inflammatory mediators in epilepsy patient plasma and controls. Patient samples were drawn from in-patients undergoing video-EEG monitoring, without regard to timing of seizures. Differences were defined by t-test, and Receiver Operating Condition (ROC) curves determined the ability of these tests to distinguish between the two populations. In epilepsy patient plasmas, we found that concentrations of anti-inflammatory sICAM5 are reduced (p = 0.002) and pro-inflammatory IL-1β, IL-2, and IL-8 are elevated. TARC (thymus and activation regulated chemokine, CCL17) concentrations trend high. In contrast, levels of BDNF and a variety of other pro-inflammatory mediators are not altered. Based on p-value and ROC analysis, we find that the ratio of TARC/sICAM5 discriminates accurately between patients and controls, with an ROC Area Under the Curve (AUC) of 1.0 (p = 0.034). In conclusion, we find that the ratio of TARC to sICAM5 accurately distinguishes between the two populations and provides a statistically and mechanistically compelling candidate blood biomarker for drug resistant epilepsy.

show abstract

Section: Discussionmentioning

confidence: 99%

The TARC/sICAM5 Ratio in Patient Plasma is a Candidate Biomarker for Drug Resistant Epilepsy

Pollard¹,

Eidelman²,

Mueller³

et al. 2013

Front. Neur.

View full text Add to dashboard Cite

show abstract

“…ICAM-4, a red cell membrane glycoprotein originally named Landsteiner-Wiener (LW) blood group antigen [15], is still being investigated for its physiological role in binding to LFA-1, but may be involved in erythropoesis [10]. ICAM-5 (telencephalin), which is expressed by neurons, is suggested to act as an adhesion molecule for leukocyte trafficking in the central nervous system [9]. JAM-1 is a recently discovered ligand for LFA-1 and plays an important role in the sequential steps of adhesion and transmigration during the recruitment of memory T cells and neutrophils [11].…”

Section: Lfa-1mentioning

confidence: 99%

Leukocyte integrins and their ligand interactions

2009

View full text Add to dashboard Cite

Although critical for cell adhesion and migration during normal immune-mediated reactions, leukocyte integrins are also involved in the pathogenesis of diverse clinical conditions including autoimmune diseases and chronic inflammation. Leukocyte integrins therefore have been targets for anti-adhesive therapies to treat the inflammatory disorders. Recently, the therapeutic potential of integrin antagonists has been demonstrated in psoriasis and multiple sclerosis. However, current therapeutics broadly affect integrin functions and, thus, yield unfavorable side effects. This review discusses the major leukocyte integrins and the anti-adhesion strategies for treating immune diseases.

show abstract

“…All 5 clones were 100% identical to ICAM-5 sequence. ICAM-5 has been suggested to play a crucial role in CNS immune modulation (Tian et al, 2000), and has also been used as a marker for acute HSV encephalitis (Lindsberg et al, 2002). To confirm the binding of UOL to ICAM-5, GST pull down assays were performed, in which GST-UOL fusion protein was incubated with brain lysates infected with wild type virus and ΔUOL virus, respectively (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…ICAM-5 or telencephalin, an immune modulator in the CNS (Kilgannon et al, 1998; Tian et al, 2000), was found to partner with UOL protein. Interestingly, soluble ICAM-5 has been detected in experimental hypoxic-ischemic injury of the brain (Guo et al, 2000) and herpes simplex encephalitis (Lindsberg et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…To our surprise, levels of cytokines/chemokines were higher in mouse brains infected with wild type virus than with ΔUOL virus. Using mouse brain cDNA library as the prey target and the UOL as bait in the BacterioMatch 2-hybrid system, we found that UOL bound ICAM-5, a molecule that has been shown to play an important immune modulation role in CNS (Tian et al, 2000). In mouse brains infected with wild type virus, expression of ICAM-5 was reduced at early time points of infection and returned to normal when infectious HSV-1 virus was undetectable, while the levels of ICAM-5 remained the same during the course of ΔUOL virus infection.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

ICAM-5 modulates cytokine/chemokine production in the CNS during the course of herpes simplex virus type 1 infection

Tse

Lane

Mott

et al. 2009

Journal of Neuroimmunology

View full text Add to dashboard Cite

Chemokines are important in HSE development in the CNS but underlying regulatory events are unknown. Two-hybrid binding assays identified that intercellular adhesion molecule 5 (ICAM-5), an immune modulator in the CNS, interacted with neurovirulence factor, UOL, of HSV-1. Viral load and interleukin levels were similar in UOL deletion virus (ΔUOL), and wild type virus infected mouse brains. However, higher numbers of lymphocytes, but unaltered soluble ICAM-5 and chemokine levels were detected in ΔUOL infected mouse brains. In contrast, lower lymphocyte numbers, reduced soluble ICAM-5, and higher chemokine levels were detected in wild type virus infected brains. Our results suggest that ICAM-5 plays a critical role in modulating chemokine production in the CNS.

show abstract

Binding of T lymphocytes to hippocampal neurons through ICAM-5 (telencephalin) and characterization of its interaction with the leukocyte integrin CD11a / CD18

Cited by 52 publications

References 34 publications

The TARC/sICAM5 Ratio in Patient Plasma is a Candidate Biomarker for Drug Resistant Epilepsy

The TARC/sICAM5 Ratio in Patient Plasma is a Candidate Biomarker for Drug Resistant Epilepsy

Leukocyte integrins and their ligand interactions

ICAM-5 modulates cytokine/chemokine production in the CNS during the course of herpes simplex virus type 1 infection

Contact Info

Product

Resources

About