Bioaccumulation of heavy metals in the earthworms Lumbricus rubellus and Aporrectodea caliginosa in relation to total and available metal concentrations in field soils

Hobbelen, P.H.F.; Koolhaas, Josée E.; Gestel, Cornelis A.M. van

doi:10.1016/j.envpol.2006.01.019

Cited by 198 publications

(85 citation statements)

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“…While L. violaceus is a limicolous (marsh dwelling) species that rarely migrates from its habitat, E. eugeniae by nature is gregarious and this may have prevented bioaccumulation of these heavy metals to such higher concentrations as observed in L. violaceus. This observation agreed with the study of Hobbelen et al (2006) which showed that the accumulation behavior of heavy metals in earthworms and their sensitivity to these pollutants, vary from species to species.…”

Section: Discussionsupporting

confidence: 80%

Glutathione-S-Transferase Production in Earthworm as Tool for Assessment of Heavy Metal Pollution in Abattoir Soil

Owagboriaye

Dedeke

Ademolu

2016

RBT

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Abstract:The use of direct response of animals to environmental challenges by production of biomarkers is a better tool to assess environmental pollution than the conventional methods. This study aimed to measure Glutathione-S-transferase (GST) in earthworms as tools for assessing heavy metal pollution in abattoir soil. Five (5) replicates each of earthworm species (Libyodrilus violaceous, Eudrilus eugeniae and Alma millsoni), soil and rumen waste samples were collected from three (3) abattoir sites (Lafenwa, Gbonogun and Madojutimi abattoirs), and a control site located within Federal University of Agriculture Abeokuta, beside an undisturbed stream with no rumen waste. Heavy metal (Cu, Zn, Pb, Cd, Co, Cr, Ni and Mn) concentrations in rumen waste, abattoir soils and earthworm tissues were determined using Atomic Absorption Spectrophotometer. The pH and organic matter (OM) concentrations of the rumen waste and abattoir soils were determined by standard methods. GST activities in the earthworm tissues were determined through the conjugation of 1 mM reduced glutathione (GSH) with 1 mM 1-chloro-2,4-dinitrobenzene (CDNB). The rumen waste recorded significantly higher (p ≤ 0.05) % OM, heavy metal concentrations and pH level than in their respective abattoir soils. The mean heavy metal concentrations of Cu, Zn, Pb, Cd and Mn were highest in the tissue of earthworm species obtained from Lafenwa abattoir. A significantly (p ≤ 0.05) higher GST activities were recorded in the tissue of earthworm species obtained from Lafenwa and Gbonogun abattoirs. Libyodrilous violaceus obtained from Lafenwa abattoir recorded the highest GST activity (8.47±1.39) in their tissue followed by the ones from Gbonogun abattoir (8.21±0.85). A significant (p ≤ 0.05) positive correlations was observed between GST activities in earthworm tissues and heavy metal concentrations. GST activities can therefore be used to assess the level of heavy metal pollution in abattoir soils. Rev. Biol. Trop. 64 (2): 779-789. Epub 2016 June 01.

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Section: Discussionsupporting

confidence: 80%

Glutathione-S-Transferase Production in Earthworm as Tool for Assessment of Heavy Metal Pollution in Abattoir Soil

Owagboriaye

Dedeke

Ademolu

2016

RBT

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“…Bioavailability and bioaccumulation of metals depend on factors such as the dimension and its concentration (Hobbelen et al, 2006Heggelund et al, 2014. Vol.…”

Section: Wwwccsenetorg/masmentioning

confidence: 99%

Impact of Zn Nanoparticles on Growth, Survival and Activity of Antioxidant Enzymes in Eisenia Fetida

Лебедев¹,

Yausheva²,

Galaktionova³

et al. 2015

MAS

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Biotesting of nanomaterials assumes greater significance and includes various biological models. The research objective is to study the influence of different concentrations of nanoparticles (NP) of Zinc (Zn) on propagation, survival and activity of antioxidatic enzymes of metal in Eisenia fetida in artificial soil (AS) and microcrystalline cellulose (MCC). Zn nanoparticles in size of 90.7±0.3 nМ, Z-potential 25±0,5мV have been used for the analysis. Artificial soil and microcrystalline cellulose were used as substrates for the study. Zn nanoparticles in high concentrations (from 500 to 1000 mg/kg) cause immobilization of 60% of earthworms within 24 hours. The highest accumulation of metal in earthworms and high mortality were registered at a concentration of 500 mg/kg in microcrystalline cellulose and at 1000 mg/kg in artificial soil.It was established that after the increase in concentration from 50 to 500 mg/kg the activity of GPx and SOD in two substrates increases. The highest activity occurs when Zn nanoparticles have concentration of 500 mg/kg. The activity of catalase in worms reduced at the exposition in MCC, but it increased after increasing dosage of Zn NPs in the artificial soil.The study demonstrates that Zn nanoparticles in dose of 500 mg/kg induced the highest toxical effect. It was proven by behavioral reactions, growth characteristics and indices of enzymatic activity in organism of redworm. Based on the obtained data, substrate from microcrystalline cellulose can be used as experimental.

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“…A number of studies have shown that earthworms can survive in heavy metal-contaminated sites (Langdon et al, 1999(Langdon et al, , 2001Reinecke, 1999;Reid and Watson, 2005;Arnold et al, 2008) and accumulate heavy metals (van Gestel et al, 1993;Darling and Thomas, 2005;Hobbelen et al, 2006) including Cd, which is a powerful toxic agent to living organisms. The mechanisms involved in heavy metal tolerance may be explained by the presence of metalbinding proteins, which have been proven for many species and probably play a critical role in storing a substantial body burden of metals (Dallinger, 1996;Fraysse et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Cloning, characterization, and expression of cadmium-induced metallothionein-2 gene from earthworm Pheretima aspergillum (E. Perrier)

Gong¹,

Li²,

Li³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Metallothioneins (MTs) are ubiquitous metal-binding, cysteine-rich proteins, associated with metal accumulation and thus providing protection against toxic heavy metals such as cadmium (Cd). To investigate the mechanisms of enrichment of Cd in the earthworm Pheretima aspergillum, we isolated and cloned metallothionein-2 (MT-2) cDNA (538 bp) from P. aspergillum, analyzed its sequence, and examined MT-2 transcription levels by relative quantitative real-time PCR under different concentrations of Cd. The sequence of P. aspergillum MT-2 cDNA and its putative amino acid sequence were highly similar to sequences from other earthworms. The induction with Cd increased the MT-2 gene transcription level in a dose-dependent manner. In addition, earthworm recombinant MT-2 exhibited high Cd bioaccumulation ability in vitro. These results suggested that MT-2 plays an important role in tolerance and accumulation of Cd in P. aspergillum.

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Bioaccumulation of heavy metals in the earthworms Lumbricus rubellus and Aporrectodea caliginosa in relation to total and available metal concentrations in field soils

Cited by 198 publications

References 31 publications

Glutathione-S-Transferase Production in Earthworm as Tool for Assessment of Heavy Metal Pollution in Abattoir Soil

Glutathione-S-Transferase Production in Earthworm as Tool for Assessment of Heavy Metal Pollution in Abattoir Soil

Impact of Zn Nanoparticles on Growth, Survival and Activity of Antioxidant Enzymes in Eisenia Fetida

Cloning, characterization, and expression of cadmium-induced metallothionein-2 gene from earthworm Pheretima aspergillum (E. Perrier)

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