Bioactive compound of Streptomyces capoamus as biocontrol of Bacterial Wilt Disease on Banana Plant

Kawuri, Retno; Darmayasa, I B G

doi:10.1088/1755-1315/347/1/012054

Cited by 8 publications

(2 citation statements)

References 6 publications

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“…Both compounds have previously been reported to have antimicrobial activities against pathogenic bacteria. (Kawuri and Darmayasa, 2019). The relatively high concentration of the alkaloid piperine could account for the antibacterial activity exhibited against E. coli.…”

Section: Identification Of Bioactive Compoundsmentioning

confidence: 99%

UV-mediated enhancement of antibacterial secondary metabolites in endophytic Lasiodiplodia theobromae

et al. 2022

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In the science of drug discovery, ultraviolet (UV) irradiation has been applied to induce mutagenesis in fungi to provide possibilities for the stimulation or enhancement of fungal biosynthetic capabilities. This study was carried out to evaluate the effect of UV radiation on the biosynthesis of antibacterial secondary metabolites in an endophytic Lasiodiplodia theobromae. Using standard methods, the fungus was isolated from healthy leaves of Cola acuminata and identified based on PCR amplification and genomic sequencing of the internal transcribed spacer (ITS) region. Cultures of L. theobromae were exposed to UV radiation at different time intervals of 1, 2 and 5 min. The fungus was subjected to solid-state fermentation in rice medium before and after UV treatments. The fungal secondary metabolites were extracted and tested for antibacterial activity using the agar diffusion method. Compounds present in the obtained extracts were identified by HPLC and GC-MS analysis. At a concentration of 1 mg/ml, the extract of the wild type L. theobromae (untreated) was observed to only inhibit Staphylococcus aureus, with an IZD of 12 mm. However, the extract of UV-treated L. theobromae (2 min) inhibited S. aureus, Escherichia coli and Pseudomonas aeruginosa with an IZD of 10 and 4 mm respectively. A wide array of compounds in the phenolics, fatty acids, alkaloids and alkanes classes were identified in the UV-treated and untreated fungal extracts. Overall, UV treatments of L. theobromae stimulated the production of seventeen (17) new compounds that were not detected in the untreated strain. The study confirms UV irradiation as an effective method for stimulating microbial biosynthesis of new bioactive compounds, indicating a promising and potentially abundant source of new drug compounds from microorganisms.

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Section: Identification Of Bioactive Compoundsmentioning

confidence: 99%

UV-mediated enhancement of antibacterial secondary metabolites in endophytic Lasiodiplodia theobromae

et al. 2022

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“…The long chain hydrocarbon, hexadecane from Jatropha curcas leaf extracts exhibited against groundnut late leaf spot disease caused by Phaeosariopsis personata (Francis et al, 2021). Hexadecanoic acid-methyl ester, a long chain fatty ester produced from Streptomyces galbus TP2 and Streptomyces humidus, has been identified as an antifungal constituent (Kawuri & Darmayasa, 2019). It should be noted that bis(2-ethylhexyl)-hexanedioic acid, the major compound in DR7-3 extract, possessed antimicrobial, antioxidant, and antiproliferative activities (Kadhim et al, 2017) (Paramanantham & Murugesan, 2014).…”

Section: Identification Of Bioactive Compounds Of Strain Dr7-3 By Gc-msmentioning

confidence: 99%

Identification, antifungal and plant growth promoting activities of endophytic actinomycetes from orchids

Tedsree

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Sixty-two endophytic actinomycetes were isolated from 15 Thai orchids. They were identified as Streptomyces (34 isolates), Micromonospora (21 isolate), Streptosporangium (2 isolates), Actinomadura (1 isolates), Amycolatopsis (3 isolates) and Pseudonocardia (1 isolates) based on their phenotypic, chemotaxonomic and genotypic characteristics. The antimicrobial activity screening revealed that Streptomyces (18 isolates), Micromonospora (12 isolates) and Streptosporangium (1 isolate) exhibited antimicrobial activity against Bacillus subtilis ATCC 6633, Kocuria rhizophila ATCC 9341, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 10231. Strain DR2-2 could inhibit all tested pathogens. Based on 16S rRNA gene sequencing, strain DR6-1 was closely related to Amycolatopsis nivea KCTC 39515T with 99.06%, average nucleotide identity (ANI) with 93.8 % and digital DNA-DNA hybridization (dDDH) with 52.50 %. Therefore, strain DR6-1T was a new actinomycete species and was proposed as Amycolatopsis dendrobii. Based on 16S rRNA gene sequencing, strain DR5-3 was closely related to Micromonospora yasonensis DSM 45980T (98.96%), Therefore, strain DR5-3T was a new actinomycete species and was proposed as Micromonospora dendrobii. Strains DR5-1, DR7-3, DR8-5, and DR8-8 showed inhibitory activity against five phytopathogenic fungi including Fusarium oxysporum SA01, Fusarium solani SA02, Alternaria alternata SA01, Curcuria oryzae SA04, and Colletotrichum gloeosporioides SA03. Strain DR7-3 exhibited a broad-spectrum antifungal activity against five fungi, especially C. oryzae SA04. The analysis of secondary metabolites from ethyl acetate extract of strain DR7-3 using the gas chromatography-mass spectrometry, fifteen identified compounds were found. Eicosane, phenol-2,4-bis(1,1-dimethylethyl), hexadecane, and hexadecanoic acid-methyl ester showed antifungal activity. �Screening for plant-growth promoting activity, endophytic actinomycetes could produce Indole-3-acetic acid (IAA) ranged from 0.04?0.36 to 294.10?12.17 ?g/mL. Strain DR1-2 showed maximum IAA of 489.73?8.90 ?g/mL, when optimized using 0.5% L-tryptophan, pH 6, with incubation at 30?C for 7 days. The IAA of strain DR1-2 enhanced the root length, shoot length, the number of roots, and fresh weight of rice seedlings (Oryza sativa L. cv. RD49). Results indicated that actinomycetes from Thai orchids were promising sources of antimicrobial compounds and plant hormones for agricultural applications.

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Preparation of biofunctionalized silver nanoparticles using Clerodendrum glandulosum leaf extract for evaluation of its antibacterial efficacy

Dhara,

Khatun,

Mondal

et al. 2024

Chem. Pap.

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Bioactive compound of Streptomyces capoamus as biocontrol of Bacterial Wilt Disease on Banana Plant

Cited by 8 publications

References 6 publications

UV-mediated enhancement of antibacterial secondary metabolites in endophytic Lasiodiplodia theobromae

UV-mediated enhancement of antibacterial secondary metabolites in endophytic Lasiodiplodia theobromae

Identification, antifungal and plant growth promoting activities of endophytic actinomycetes from orchids

Preparation of biofunctionalized silver nanoparticles using Clerodendrum glandulosum leaf extract for evaluation of its antibacterial efficacy

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