Production of Macrobrachium rosenbergii shrimp was limited by vibriosis disease. Streptomyces can be used as an alternative control of vibriosis which is caused by Vibrio anguillarum. Aplication of Streptomyces sp.1 culture could give significant different impacts (p<0.05) on the percentage of survival (SR) of shrimp larvae that had been infected by V. anguillarum compared to the control. This study was conducted to identify and determine bioactive compound of Streptomyces sp.1. The presence of bioactive compound was confirmed by gas chromatography-mass spectrometry (GC-MS) analysis. Identification of Streptomyces sp.1 by 16S rRNA partial gene sequencing method. Streptomyces sp.1 was identified to be Streptomyces sp.Sp1. There were 35 chemical compounds identified, the chemical compounds which have antimicrobial properties identified in the n butanol extract of Streptomyces sp.Sp1 are 3-Hexanone, 2-methyl (43%), n Butyl ether (19%) Nonane (CAS) n-Nonane (5.6%), Decane (CAS) n-Decane (3.2%), Hexacosane (CAS) n-Hexacosane (1.9%), Tetracontane (1.3%), Heneicosane (1.9%), Hexadeconoic acid, methyl ester (0.3%), Butane,1,1-dibutoxy (0.3%), and Limonene (0.3%). Benzeneacetic acid, 3-methoxy-.alpha.,4-bis[(tri (0.3%). These results suggested that Streptomyces sp.Sp1 had good general antimicroba activity and might have potential biocontrol antagonist Vibrio anguillarum.
Banana is a plant that has a high economic value for human to fulfil his daily fruits need.. The antagonistic test between nine isolates Streptomyces sp. and R. solanacearum showed that the Streptomyces sp. 9 has the highest resistance (19 mm), indicating a strong inhibition and has smallest concentrations of inhibition (MIC) of 10%. The glasshouse scale test demonstrated that the Streptomyces sp. 9 culture sprayed 4 times on a different day could suppress bacterial wilt disease by 100% from 11% in control. The research was to determine molecular identification of Streptomyces sp.9 using 16S rRNA and identification of active compound by Gass Chromatography Mass Spectra (GCMS). The results showed that the molecular identified Streptomyces sp.9 as a Streptomyces capoamus. The chemical compounds which have antimicrobial are Hexadeconoic acid methyl ester, Heneicosane, Hexacosane(CAS) n-Hexacosane, Limonene, Dotriacontane and Tetracontane as antimicrobial agent. The conclusion was Streptomyces capoamus showed various bioactive compounds, which is importance as potential biocontrol on bacterial wilt disease on banana plants. There could be probably of new bioactive compounds and this also provided a new insight towards the development of good candidates for bioactive natural products.
Acinobacter baumanii is one of the pathogenic bacteria that has several antibiotic resistance. Streptomyces is a potential bacteria that can produce several antibiotics. Aim of this research were to determine chemical compound and analysis in silico extract of n butanol Streptomyces drozdowiczii. Streptomyces drozdowiczii showed the presence of 10 peaks identified as Cyclohexene, 1-Methoxy-1-buten-3-yne, Butanenitrite, n-Butyronitrite, 1-Pentane-3-yne-2-methyl, 3,3,5-Trimethylcyclohexylamine, 9-Borabicyclo (421) nonane-dimer and 3-Methyl-2-oxo-2-pyrane-6-carboxylic acid. Analisis in silico showed an interaction occurs between 5 compounds of Streptomyces that function as antimicrobial agents. The conclusion n butanol extract of S.drozdowiczii has a potential as new antibiotic against A. baumanii
Acinobacter baumannii multi drug resistant (MDR) bacteria are recognized as one of the aerobic bacteria resistant to some antibiotics.. The aim of this study is to find appropriate doses of antibiotics used in white mice infected with MDR A baumanni; toxicity liver organ and histopathology of treated white mice. Mice was infected with A baumanii one day before treatment. Antibiotic Streptomyces drozdowiczii dose treatment with concentration 7%, 6%, 5%, 4% and control (without treatment) and positive control with ciprofloxacin.. Blood cuture is taken after 5 days to determine total bacteria A baumanii and liver funtion. Liver histopathology analysis is done by histological incisions with paraffin and staining methods. The results showed that % of live animals were antibiotic concentrations of 4% 5%, 6%, 7%, positive controls and negative controls, ie sequentially 40%, 100%, 100%, 100%, 100% and 100%. All animals did not show liver damage from SGOT and SGPT analyzes. Histopathological analysis showed liver cells in animals tried positive control, negative control and administration of 7% antibiotic showed normal liver cells, Conclusion; 7% antibiotic is the best by not damaging the liver and A baumanii bacteria is not present in the blood.
Research on weed inventory in maize land aims to see the diversity of existing weeds growing on the area of ??corn (Zea mays (L.)) in the fields of Bongkasa Abiansemal Badung Village, Bali Province. This observation will be expected to obtain information about the types of weeds that exist, so that weeds and control of weeds in corn (Zea mays (L.)) are optimally implemented. The study was conducted from June to August 2018. In observations using an exsplorating sampling method. In order to produce better and more systematic results, observations were made to make lanes and observations on the research land so that it could easily observe the growth of disturbing plants growing on the observed maize crops. In a 1-month old corn plant, 8 tribes were found with 16 species. In the 2month old corn plant, 14 tribes were found with 28 species of weeds, while in the 3-month-old maize there were 22 tribes with 40 species of weeds. The 22 tribes are Amaranthaceae, Asteraceae, Balsaminaceae, Capparidaceae, Commelinaceae, Convolvulaceae, Cyperaceae, Euphorbiaceae, Goodeniaceae, Laminaceae, Mimosaceae, Loganiaceae, Malvaceae, Onagraceae, Oxalidaceae, Poaceae, Portulacaceae, Rubiaceae, Scrophulariaceae, Solanaceae, Urtcaceae, Violaceae, Urtcaceae, with 40 species namely Amaranthus gracilis (Desf), Amaranthus spinosus (L), Ageratum conoyzoides L., Eclipta alba L., Emilia sonchifolia (L.), Vernonia cinerea (L, Synedrella nodiflora (L.), Tridax procumbens, Impatatiens platypetalaL Cleome rutidosperma DC, Cleome viscosaL, Murdania nudiflora (L.) Brenan, Impomea triloba L., Paedenia foetida L., Impomea gassicaulis Rot, Cyperus rotundus L. Kylliangia monocephala (Endl). Euphorbia hirta L. Phyllantus urinaria L., Phyllantus debilis Klein. Elephantopus scaber C., Basilicum polystachyon (L.), Mimosa pudica (L). Spigelia anthelmia L., Sida rhombifolia L., Ludwigia perennis L., Oxalis corniculata L. Dactyloctenium aegy ptium (L.), Digitaria ciliaris (Retz.), Eragrotis tenella (L.), Echinoclhoa colonum (L.), Elusine indica L., Portulaca oleraceae L., Hedyotis diffusa (L.) Lamk, Lindernia crustacea (L.), Scoparia dulcis L., Physalis angulata L., Capsicum frutescens, Urtica grandidentata L, Hybanthus attenuates L. Keywords: weed, Zea mays L., Bongkasa Village.
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