Bioactive Contaminants Leach from Disposable Laboratory Plasticware

McDonald, G. Reid; Hudson, Alan L.; Dunn, Susan M. J.; You, Haitao; Baker, Glen B.; Whittal, Randy M.; Martin, Jonathan W.; Jha, Amitabh; Edmondson, Dale E.; Holt, Andrew

doi:10.1126/science.1162395

Cited by 203 publications

(149 citation statements)

References 5 publications

Supporting

Mentioning

142

Contrasting

Unclassified

Order By: Relevance

“…Even when the highest-quality reagents and plasticware are used, the introduction of some unknown substances (contaminants) into the culture environment is unavoidable [1][2][3]. As a result, all supplies that will come into contact with the embryo and/or the culture medium should be subjected to strict quality control (QC) testing, including the ability to support the growth of murine embryos [4,5].…”

Section: Introductionmentioning

confidence: 99%

Building a better mouse embryo assay: effects of mouse strain and in vitro maturation on sensitivity to contaminants of the culture environment

Herrick

Paik

Strauss

et al. 2015

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The aim of this study is to compare the sensitivity of the standard one-cell mouse embryo assay (MEA) to that using in vitro-matured oocytes from hybrid and outbred mice. Methods The study was done by culturing embryos in the presence or absence of two concentrations (0.0005 or 0.001 %v/v) of Triton X-100 (TX100). Embryonic development, blastocyst cell numbers (total and allocation to the trophectoderm [TE] and inner cell mass [ICM]), and blastocyst gene expression were evaluated. Results Neither concentration of TX100 affected (P>0.05) cleavage, blastocyst development, or hatching in one-cell embryos from BDF1 mice. However, all cell number endpoints were reduced (P<0.05) by the high concentration of TX100 and the number of ICM cells was reduced (P<0.05) by the low concentration of TX100. Inhibitory (P<0.05) effects of the high concentration of TX100 were observed in in vitro maturation (IVM) embryos from BDF1, CF1, and SW, but not ICR, mice. Cell number and allocation were negatively affected by the high concentration of TX100 in CF1 and SW embryos, but not in BDF1 or ICR embryos. The only developmental endpoints affected by the low concentration of TX100 were cleavage of BDF1 oocytes, blastocyst development of SW embryos, and cell numbers (total and inner cell mass (ICM)) of SW blastocysts. Conclusions The sensitivity of the MEA to TX100 is improved by using embryos from in vitro-matured oocytes, using oocytes from some outbred (SW or CF1, not ICR) strains of mice, and evaluating blastocyst cell number and allocation.

show abstract

Section: Introductionmentioning

confidence: 99%

Building a better mouse embryo assay: effects of mouse strain and in vitro maturation on sensitivity to contaminants of the culture environment

Herrick

Paik

Strauss

et al. 2015

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…N-arachidonoylglycine-d 8 , 1 pmole per 10 l injection, was spiked into each extraction to measure instrument performance and for data normalization. Solvent and slip additive blanks were run to evaluate background oleamide and palmitamide levels (29). In addition, blank samples were run between standards and extracts to eliminate memory effects from the HPLC column.…”

Section: Identification and Quantification Of Endogenous Long-chain Fmentioning

confidence: 99%

Glycine N-acyltransferase-like 3 is responsible for long-chain N-acylglycine formation in N18TG2 cells

Jeffries

Dempsey²,

Farrell³

et al. 2016

Journal of Lipid Research

View full text Add to dashboard Cite

The long-chain fatty acid amides are an emerging family of bioactive lipids with members that include N-acyl amino acids, primary fatty acid amides (PFAMs), N-acylarylalkylamides, N-acylethanolamines, and N-monoacylpolyamines. Fatty acid amides were first identified from biological sources over 50 years ago with the isolation and identification of N-palmitoylethanolamine from egg yolk in 1957 (1) and, in 1965, N-palmitoylethanolamine and N-stearoylethanolamine in several tissues from rat and guinea pig (2). The discovery of N-arachidonoylethanolamine (anandamide) as the endogenous ligand for the mammalian brain cannabinoid receptor CB 1 sparked a dramatic interest in the fatty acid amides (3). Since these early discoveries, a diversity of long-chain fatty acid amides have been identified in mammals and, more recently, in invertebrates as well (4)(5)(6)(7)(8).The N-fatty acylglycines, a subclass of the N-fatty acyl amino acids, are an important class of cell signaling lipids that are distributed throughout the central nervous system and the rest of the body (6, 7, 9). Identification of glycine conjugates dates back to the 1820s with the identification of N-benzoylglycine (hippurate) as a mammalian metabolite (10). N-arachidonoylglycine was the first longchain N-acylglycine identified from a mammalian source and was determined to have anti-nociceptive and antiinflammatory effects in rat models of pain (11). Since

show abstract

“…Desorption of contaminants has been shown to compromise biological assay data quality. 1,2 The most common way to make a concentration gradient is via serial dilutions of a stock solution in sequential steps. One well-known problem in generating a concentration gradient is due to the compounding of error resulting from multiple transfer steps.…”

mentioning

confidence: 99%

Gradient, Contact-Free Volume Transfers Minimize Compound Loss in Dose-Response Experiments

Harris¹,

Olechno²,

Datwani³

et al. 2010

SLAS Discovery

View full text Add to dashboard Cite

More accurate dose-response curves can be constructed by eliminating aqueous serial dilution of compounds. Traditional serial dilutions that use aqueous diluents can result in errors in dose-response values of up to 4 orders of magnitude for a significant percentage of a compound library. When DMSO is used as the diluent, the errors are reduced but not eliminated. The authors use acoustic drop ejection (ADE) to transfer different volumes of model library compounds, directly creating a concentration gradient series in the receiver assay plate. Sample losses and contamination associated with compound handling are therefore avoided or minimized, particularly in the case of less water-soluble compounds. ADE is particularly well suited for assay miniaturization, but gradient volume dispensing is not limited to miniaturized applications.

show abstract

Bioactive Contaminants Leach from Disposable Laboratory Plasticware

Cited by 203 publications

References 5 publications

Building a better mouse embryo assay: effects of mouse strain and in vitro maturation on sensitivity to contaminants of the culture environment

Building a better mouse embryo assay: effects of mouse strain and in vitro maturation on sensitivity to contaminants of the culture environment

Glycine N-acyltransferase-like 3 is responsible for long-chain N-acylglycine formation in N18TG2 cells

Gradient, Contact-Free Volume Transfers Minimize Compound Loss in Dose-Response Experiments

Contact Info

Product

Resources

About