Bioactive Nanoparticles Synthesized By Green Method

Çiftçi, Harun; Çalışkan, Çiğdem Er; Öztürk, Kübra; Yazici, Burcu

doi:10.34248/bsengineering.816084

Cited by 5 publications

(1 citation statement)

References 155 publications

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“…The leaves of the plant have medicinal uses with their positive effect in the treatment of paralysis, skin burns, stopping bleeding, and frostbite. It is chemical composition is rich in variable bioactive components such as phenolic compounds, flavonoid oligomers, tannins, ascorbic acid, and caffeine ( Çifçi et al, 2021 ). In this work, AgNPs were produced utilizing the aqueous extract of D. kaki leaf.…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of silver nanoparticles mediated Diospyros kaki L. (Persimmon): determination of chemical composition and evaluation of their antimicrobials and anticancer activities

Keskin,

Ölçekçi,

Baran

et al. 2023

Front. Chem.

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The eco-friendly synthesis of metallic nanoparticles (MNPs) using biological materials is an encouraging and innovativeness approach to nanotechnology. Among other synthesizing methods, biological methods are chosen because of their high efficiency and purity in many aspects. In this work, using the aqueous extract obtained from the green leaves of the D. kaki L. (DK); silver nanoparticles were synthesized in a short time and simply with an eco-friendly approach. The properties of the synthesized silver nanoparticles (AgNPs) were characterized using various techniques and measurements. In the characterization data of AgNPs, Maximum absorbance at 453.34 nm wavelengths, the average size distribution of 27.12 nm, the surface charge of −22.4 mV, and spherical appearance were observed. LC-ESI-MS/MS analysis was used to assess the compound composition of D. kaki leaf extract. The chemical profiling of the crude extract of D. kaki leaves revealed the presence of a variety of phytochemicals, predominantly phenolics, resulting in the identification of five major high-feature compounds: two major phenolic acids (Chlorogenic acid and Cynarin), and tree flavonol glucosides (hyperoside, quercetin-3-glucoside, and quercetin-3- D-xyloside). The components with the highest concentrations were cynarin, chlorogenic acid, quercetin-3- D-xyloside, hyperoside, and quercetin-3-glucoside, respectively. Antimicrobial results were determined by a MIC assay. The biosynthesized AgNPs exhibited strong antibacterial activity against the human and food pathogen Gram (+ and −) bacteria and good antifungal activity against pathogenic yeast. It was determined that 0.03–0.050 μg/mL concentrations ranges of DK-AgNPs were growth suppressive concentrations on all pathogen microorganisms. The MTT technique was used to study the cytotoxic effects of produced AgNPs on cancer cell lines (Glioblastoma (U118), Human Colorectal Adenocarcinoma (Caco-2), Human Ovarian Sarcoma (Skov-3) cancer cell lines, and Human Dermal Fibroblast (HDF) healthy cell line). It has been observed that they have a suppressive effect on the proliferation of cancerous cell lines. After 48 h of treatment with Ag-NPs, the DK-AgNPs were found to be extremely cytotoxic to the CaCo-2 cell line, inhibiting cell viability by up to 59.49% at a concentration of 50 g mL−1. It was found that the viability was inversely related to the DK-AgNP concentration. The biosynthesized AgNPs had dose-dependent anticancer efficacy. Because of the high concentration of bioactive chemicals in Diospyros kaki, it may be employed as a biological resource in medicinal applications. DK-AgNPs were shown to be an effective antibacterial agent as well as a prospective anticancer agent. The results provide a potential approach for the biogenic production of DK-AgNPs utilizing D. kaki aqueous leaf extract.

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Section: Introductionmentioning

confidence: 99%

Green synthesis of silver nanoparticles mediated Diospyros kaki L. (Persimmon): determination of chemical composition and evaluation of their antimicrobials and anticancer activities

Keskin,

Ölçekçi,

Baran

et al. 2023

Front. Chem.

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Eco-Friendly Synthesis of Silver Nanoparticles Using Klebsormidium subtile and Evaluation of their Antimicrobial, Anti-Quorum Sensing, and Antibiofilm Activities

Açıkgöz Erkaya,

Yalçın,

Erdem

2024

Cumhuriyet Science Journal

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In this study, both dry and fresh biomass extracts of Klebsormidium subtile were used for the synthesis of silver nanoparticles (AgNPs). The UV-visible spectrum showed an absorption peak at 430 nm, indicating the presence of AgNPs through surface plasmon resonance. FT-IR analysis identified bioactive functional groups, such as amines, which acted as stabilizing agents for the nanoparticles. SEM imaging revealed well-dispersed, spherical AgNPs ranging from 5 to 25 nm and 40 to 60 nm in size, accumulating on cell surfaces. EDS analysis confirmed the presence of elemental silver. The antimicrobial activity of AgNPs derived from both fresh and dry K. subtile extracts was similar, though AgNPs from the dry extract were more effective against Staphylococcus aureus, with inhibition zones of 15.8, 16.2, and 15.2 mm at 1 mM, 2 mM, and 3 mM concentrations, respectively. AgNPs also showed strong activity against Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans, but were less effective against Bacillus cereus and Aeromonas hydrophila. These findings suggest that K. subtile-derived AgNPs have significant antimicrobial potential, particularly against S. aureus and C. albicans, and may be useful in biomedical applications, particularly for treating biofilm-related infections.

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Primula vulgaris'ten Sentezlenen Gümüş Nanopartiküllerin Antimikrobiyal ve Antioksidan Potansiyeli

Erdem

Çiftçi

ŞAHİN>

2022

Muş Alparslan Üniversitesi Fen Bilimleri Dergisi

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In this study, biosynthesis and in vitro phytochemical composition, antibacterial and antioxidant activities of silver nanoparticles were investigated by using aqueous leaf, flower and root extracts of Primula vulgaris (P. vulgaris subsp. vulgaris). The synthesized silver nanoparticles (AgNPs) were confirmed by color conversion and ultraviolet-visible (UV-visible) spectrophotometry. The appearance of a dark brown color and a UV absorption spectrum range at 440 nm confirmed the synthesized silver nanoparticles. The antimicrobial activity of silver nanoparticles synthesized from the leaf of P. vulgaris; S. aureus 25±1, S. epidermidis 20±1, P. aeruginosa 20±1, A. hydrophila 21±1, C. albicans 25±1, C. tropicalis 25±1, C. parapsilosis 22±1 and C. glabrata 20±1 mm zone diameter was determined. The most antimicrobial effect of P. vulgaris leaf aqueous extract; S. aureus 20±1, S. epidermidis 18±1, A.hydrophila 15±1, P. aeruginosa 12±2, C. albicans 18±1, C. glabrata 18±1, C.tropicalis15±2, and C. parapsilosis 15±2 mm zone diameter was revealed. The presence of flavonoids, terpenoids, protein, and carbohydrates was found to be higher in silver nanoparticles synthesized in the flower part of P. vulgaris, according to phytochemical screening. While saponins were detected in P. vulgaris root extracts, tannins and protein were detected in the leaf extract. The flower had the highest total phenolic extract content of the silver nanoparticle (29.08±0 mg GAE/g DW), while the leaf and root had the lowest total phenolic content of 9.06±0.5 and 8.64±3.3 mg GAE/g DW, respectively. The flower had the highest total phenolic extract content of the plant aqueous extracts (25.10±0.2 mg GAE/g DW), while the leaf and root had the lowest (8.28±0.5 and 5.20±0.0 mg GAE/g DW, respectively).The DPPH (1,1-Diphenyl-2-picrylhydrazil) assay was used to assess free radical scavenging activity. The antioxidant activity of AgNPs biosynthesized using P. vulgaris flower extract was 90.6 %, while P. vulgaris flower aqueous extracts were 86.3 %. This can be concluded that silver nanoparticles synthesized using P. vulgaris flower extract are useful in the preparation of pharmacologically useful drugs.

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Bioactive Nanoparticles Synthesized By Green Method

Cited by 5 publications

References 155 publications

Green synthesis of silver nanoparticles mediated Diospyros kaki L. (Persimmon): determination of chemical composition and evaluation of their antimicrobials and anticancer activities

Green synthesis of silver nanoparticles mediated Diospyros kaki L. (Persimmon): determination of chemical composition and evaluation of their antimicrobials and anticancer activities

Eco-Friendly Synthesis of Silver Nanoparticles Using Klebsormidium subtile and Evaluation of their Antimicrobial, Anti-Quorum Sensing, and Antibiofilm Activities

Primula vulgaris'ten Sentezlenen Gümüş Nanopartiküllerin Antimikrobiyal ve Antioksidan Potansiyeli

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