Bioactive peptides laden nano and micro-sized particles enriched ECM inspired dressing for skin regeneration in diabetic wounds

Nanditha, C K; Kumar, G. S. Vinod

doi:10.1016/j.mtbio.2022.100235

Cited by 32 publications

(20 citation statements)

References 42 publications

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“…The supportive nanoscale matrix promotes increased collagen deposition in the wound bed, thereby accelerating the complete healing process through massive tissue regeneration and functional recovery. The results showed that the nanoscale mat rich in nano/particles showed the potential as an effective wound repair dressing for diabetes wounds (Nanditha and Kumar, 2022).…”

Section: Loading Active Factormentioning

confidence: 99%

“…The dressing developed according to this idea has good cell compatibility with keratinocytes and fibroblasts and enhances their cell proliferation and migration ability in vitro. The supportive nanoscale matrix mimicking ECM promotes increased collagen deposition in the wound bed, thereby accelerating the complete healing process through massive tissue regeneration and functional recovery (Nanditha and Kumar, 2022). Through the electrospinning method, researchers have manufactured polylactic acid glycolic acid/ collagen nanoscale mats, and functionalized the surface with wound healing peptides, loaded chitosan nanoparticles and micron-sized particles to form an extracellular matrix (ECM) -like structure with bionic functions (Yin et al, 2020).…”

Section: Restore the Natural Structure Of Ecmmentioning

confidence: 99%

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Effect of composite biodegradable biomaterials on wound healing in diabetes

Ren

Guo²,

Yang³

et al. 2022

Front. Bioeng. Biotechnol.

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The repair of diabetic wounds has always been a job that doctors could not tackle quickly in plastic surgery. To solve this problem, it has become an important direction to use biocompatible biodegradable biomaterials as scaffolds or dressing loaded with a variety of active substances or cells, to construct a wound repair system integrating materials, cells, and growth factors. In terms of wound healing, composite biodegradable biomaterials show strong biocompatibility and the ability to promote wound healing. This review describes the multifaceted integration of biomaterials with drugs, stem cells, and active agents. In wounds, stem cells and their secreted exosomes regulate immune responses and inflammation. They promote angiogenesis, accelerate skin cell proliferation and re-epithelialization, and regulate collagen remodeling that inhibits scar hyperplasia. In the process of continuous combination with new materials, a series of materials that can be well matched with active ingredients such as cells or drugs are derived for precise delivery and controlled release of drugs. The ultimate goal of material development is clinical transformation. At present, the types of materials for clinical application are still relatively single, and the bottleneck is that the functions of emerging materials have not yet reached a stable and effective degree. The development of biomaterials that can be further translated into clinical practice will become the focus of research.

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Section: Loading Active Factormentioning

confidence: 99%

Section: Restore the Natural Structure Of Ecmmentioning

confidence: 99%

Effect of composite biodegradable biomaterials on wound healing in diabetes

Ren

Guo²,

Yang³

et al. 2022

Front. Bioeng. Biotechnol.

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“…Samples were suspended in DMEM in sterile conditions, sonicated for 10 min and treated the cells for 24 h and 48 h. After treatment, 10 µL of MTT (5mg/mL PBS) solution was added to cells and incubated further for 3 h. After removal of the culture medium, the formazan crystals were dissolved in 200 µL IPA (Isopropyl alcohol), mixed well and the absorbance of each well was measured using ELISA plate reader (Bio-Rad) at a wavelength of 570 nm. 37 Untreated cells were taken as control with 100% viability. The drug concentration at which the growth of 50% cells was inhibited (IC 50 ) was calculated by curve fitting of the cell viability data.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Sorafenib-Entrapped, Self-Assembled Pullulan–Stearic Acid Biopolymer-Derived Drug Delivery System to PLC/PRF/5 Hepatocellular Carcinoma Model

Chirayil¹,

Kumar²

2022

IJN

Self Cite

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This study aimed to design a prototypic drug delivery system (DDS) made of an amphiphilic, pullulan (Pull)-derived biodegradable polymer for targeting the asialoglycoprotein receptor (ASGPR) overexpressed in HCC. Stearic acid (SA) was conjugated to increase the hydrophobicity of pullulan (Pull-SA). Methods: Pullulan (Pull) was linked to stearic acid (SA) after functional group modifications via EDC/NHS chemistry and characterized. Sorafenib tosylate (SRFT) was entrapped in pullulan-stearic acid nanoparticles (Pull-SA-SRFT) and its particle size, zeta potential, entrapment efficiency (EE), loading capacity (LC), and release efficiency was measured. The competence of Pull-SA-SRFT over SRFT in vitro was assessed using the ASGPR over-expressing PLC/PRF/5 hepatocellular carcinoma (HCC) cell line. This was done by studying cytotoxicity by MTT assay and chromosome condensation assay, early apoptosis by annexin-Pi staining, and late apoptosis by live-dead assay. The cellular uptake study was performed by incorporating coumarin-6 (C6) fluorophore in place of SRFT in Pull-SA conjugates. A biodistribution study was conducted in Swiss-albino mice to assess the biocompatibility and targeting properties of SRFT and Pull-SA-SRFT to the liver and other organs at 1, 6, 24, and 48 h. Results:The characterization studies of the copolymer confirmed the successful conjugation of Pull-SA. The self-assembled amphiphilic nanocarrier could proficiently entrap the hydrophobic drug SRFT to obtain an entrapment efficiency of 95.6% (Pull-SA-SRFT). Characterization of the synthesized nanoparticles exhibited highly desirable nanoparticle characteristics. In vitro, apoptotic studies urged that Pull-SA-SRFT nanoparticle was delivered more efficiently to HCC than SRFT. The cellular uptake study performed, gave propitious results in 4 hrs. The biodistribution study conducted in immunocompetent mice suggested that Pull-SA-SRFT was delivered more than SRFT to the liver when compared to other organs, and that the system was biocompatible. Conclusion: Pull-SA-SRFT is a promisingly safe, biodegradable, cell-specific nanocarrier and a potential candidate to target hydrophobic drugs to HCC.

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“…1 Bacterial proliferation and the disordered extracellular matrix (ECM) at the wound site are the major reasons for delayed healing and excess scar formation. 2,3 Unlike normal skin, scar tissue is characterized by hyperproliferation of fibroblasts and excess deposition of collagen. Scar tissue has a dense, parallel fibrous extracellular matrix that makes it more fragile than normal tissue, leading to growth restriction and permanent loss of function.…”

Section: Introductionmentioning

confidence: 99%