Bioactivities and analysis of peptides from Sumbawa horse milk generated by Bacillus thuringiensis protease

Abstract

<p class="abstrak2">Sumbawa horse milk is claimed to cure some diseases such as asthma, hypertension, diabetes and gastrointestinal disorder but its potential bioactive peptide has not been explored. The aims of this study are to evaluate bioactivities peptides from Sumbawa horse milk protein hydrolysate and to analyzethe physio-chemical properties of selected peptides. The milk protein was hydrolyzed by Bacillus thuringiensis protease, the peptide produced were sequential fractionated and then analyzed for antibacterial and antioxidant activities. The peptide fraction <3 kDa was then sequenced using LCMS-MS and the physio-chemical properties of the peptides were analyzed. The result showed that peptides fraction <3 kDa from the 30 min hydrolysis was the most active as antibacterial and more active to Gram negative bacteria. For antioxidant, scavenging activity of the fraction per µg protein/mL were 83% to ABTS and 31% to DPPH radicals. The values were similar with vitamin C 12.5 µg/mL for ABTS and 14.5 µg/mL for DPPH. Peptide <tt>HPYFYAPELLYYANK with molecular weight prediction </tt>1887.92 Da and isoelectric point 7.47 has high therapeutic index prediction (64.75). The result showed that peptides from Sumbawa horse milk hydrolyzed by Bacillus thuringiensis protease was active as antibacterial and antioxidant. Peptide <tt>HPYFYAPELLYYANK from fraction <3 kDa was potential as antibacterial.</tt></p><p><strong>Key Words:</strong> <em>Bacillus thuringiensis</em>, Bioactive Peptide, Horse Milk</p>

Characterization and Antioxidant Activity of Collagen, Gelatin, and the Derived Peptides from Yellowfin Tuna (Thunnus albacares) Skin

“…The scavenging activities against ABTS radicals of the collagen hydrolysate from milkfish (Chanos chanos) skin showed the effectiveness at 1 mg/mL [51]. However, the scavenging activity of nearly 100% was reported for the peptide from horse milk at 1 µg/mL [52]. On the other hand, the gelatin hydrolysate from skipjack scale showed the scavenging activity in the concentration range of 0.1-5.0 mg/mL [8].…”

A comprehensive review on bioactive peptides derived from milk and milk products of minor dairy species

“…In another study by Kusumaningtyas et al (2018), hydrolysis of the Sumbawa mare milk protein via Bacillus thuringenesis protease yielded hydrolysates capable of exhibiting antibacterial and antioxidant activities. The peptide fraction which was < 3 kDa in molecular size, obtained from a 30 min hydrolysis, was shown to be the most potent antibacterial fraction and more effective against Gram-negative bacteria.…”

Analisis In Silico Aktivitas antimikroba Peptida dari Susu Kuda Sumbawa

“…Peptida yang digunakan berasal dari peptida yang diperoleh dari penelitian sebelumnya (Kusumaningtyas et al 2016). Susu kuda Sumbawa dihidrolisis menggunakan protease Bacillus thuringiensis.…”

“…Semakin tinggi nilai yang diperoleh semakin tinggi aktivitas antimikrobanya. Peptida LANSTEFAK yang merupakan hasil modifikasi peptida LVNELTEFAK yang sebelumnya diprediksi meningkatkan index terapi dan aktivitas antimikrobanya, pada analisis ini LANSTEFAK diprediksi meningkat aktivitasnya dibandingkan dengan LVNELTEFAK meskipun belum dapat dimasukkan dalam kategori peptida antimikroba (Kamech et al 2012;Kusumaningtyas et al 2016 Analisis selanjutnya menggunakan CAMP R3 (Collection of anti-microbial peptides) dibuat untuk mengembangkan dan mempercepat penelitian mengenai kelompok peptida antimikroba. Peptida antimikroba mempunyai kelompok-kelompok yang masing-masing mempunyai persamaan komposisi sekuen spesifik yang dapat mengarahkan penemuan peptida antimikroba yang baru.…”