Characterization and Antioxidant Activity of Collagen, Gelatin, and the Derived Peptides from Yellowfin Tuna (Thunnus albacares) Skin

Abstract

Skin waste from tuna processing needs to be utilized, such as extraction of its collagen and gelatin. Their functional properties can be improved by enzymatic hydrolysis for conversion to peptides. Thus, the research objectives were to examine the characteristics and antioxidant activity of collagen, gelatin, and the derived peptide from yellowfin tuna skin. Collagen was extracted using 0.75 M acetic acid at 4 °C, while gelatin was prepared using 0.25% citric acid and extracted at 65 °C. Hydrolysis was carried out with 2% Alcalase, followed by fractionation with a molecular weight cut off sieve for both collagen and gelatin. Collagen yield was 22.6% with pH value of 6.63 and whiteness of 96.7%. Gelatin yield was 20.0% with pH value of 4.94 and whiteness of 51.0%. Hydrolysis for three hours resulted in 52.7% and 45.2% degree of hydrolysis for collagen and gelatin, respectively. The molecular weights of collagen peptides ranged from 2.94 to 11.93 kDa, while those of gelatin peptides ranged from 3.54 to 16,620 kDa. Antioxidant activities of these peptides were higher than those before hydrolysis. The high antioxidant activity (IC50) of collagen peptides were found in <3, 3–10, and 10–30 kDa fractions as well as in the gelatin peptides.

Golden Sea Cucumber: Identification and the Antioxidant Activity of Its Collagen Hydrolysates

“…To date, protein hydrolysates with strong antioxidant activity are rarely reported. However, low MW protein hydrolysates from yellowfin tuna skin (3-10 kDa) and the spotted Babylon snail (3-5 kDa) were reported as potent antioxidants with IC 50 of 75.94 µg/ mL and 2.69 µg/mL, respectively (Nurilmala, Hizbullah, Karnia, Kusumaningtyas, & Ochiai, 2020;Petsantad et al, 2020). Liu et al (2018) investigated that hydrolysis of the Alaska pollock protein isolate using neutrase significantly improved the DPPH free radicalscavenging activity.…”

Fish Waste: From Problem to Valuable Resource

“…In addition, Nibea japonica swim bladders [ 91 ], yellowfin tuna ( Thunnus albacares ) skin [ 92 ], Scomber japonicus bone and skin [ 93 ], shark cartilage [ 94 ] also showed antioxidant properties.…”

“…Several studies pointed out the antioxidant activity of hydrolysates of collagen and its derivatives, obtained from different fish byproducts, including Gadous macrocephaius skin [ 150 ], shark cartilage [ 94 ], yellowfin tuna ( T. albacares ) skin; particularly low-weight peptide (< 3kDa) showed higher radical scavenging properties [ 92 ]. Collagen polypeptide from tilapia skin (MW< 3000Da) had protective effects against injuries to the liver and kidneys of mice induced by d-galactose by reducing oxidative stress [ 151 ].…”

Hidrolizados De Colágeno, Seus Produtos E Suas Bioatividades: Uma Mini-Revisão

“…pode ser empregada também em outros segmentos, como na produção de filmes comestíveis e no processo de encapsulamento, técnica de interesse para produção de biomateriais e produtos biofarmacêuticos. Além disso, é de grande interesse na utilização para obtenção de peptídeos com funções biológicas variáveis de acordo com a fonte da matéria-prima (NURILMALA et al, 2020;KARAYANNAKIDIS;ZOTOS, 2014).…”