Golden Sea Cucumber: Identification and the Antioxidant Activity of Its Collagen Hydrolysates

Fawzya, Yusro Nuri; Putra, Nugrah Analiadi; Witarto, Arif Budi; Patantis, Gintung

doi:10.15578/squalen.v15i3.511

Cited by 9 publications

(10 citation statements)

References 40 publications

(56 reference statements)

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“…In particular, Neutrase-derived hydrolysate produced four bioactive peptides and showed strong DPPH radical-scavenging activity, suggesting that it was related to the presence of hydrophobic amino acids in their sequences and low molecular weights (<1 kDa). Similarly, collagen hydrolysates (molecular weight 14.4 to 25 kDa) obtained from golden sea cucumber revealed strong DPPH radical scavenging activity, where Neutrase was used to start the hydrolysis [ 59 ]. García et al [ 34 ] designed novel functional food products using protein hydrolysate (Protamex and Alcalase) obtained from Parastichopus tremulus and Holothuria forskali , which demonstrated antioxidant, antihypertensive, and anti-inflammatory properties, especially those obtained from H. forskali .…”

Section: Bioactive Compounds Of Sea Cucumbers and Their Antioxidant A...mentioning

confidence: 99%

Antioxidant Potential of Sea Cucumbers and Their Beneficial Effects on Human Health

2022

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Sea cucumbers are considered a luxury food item and used locally in traditional medication due to their impressive nutritional profile and curative effects. Sea cucumbers contain a wide range of bioactive compounds, namely phenolics, polysaccharides, proteins (collagen and peptides), carotenoids, and saponins, demonstrating strong antioxidant and other activities. In particular, phenolic compounds, mainly phenolic acids and flavonoids, are abundant in this marine invertebrate and exhibit antioxidant activity. Protein hydrolysates and peptides obtained from sea cucumbers exhibit antioxidant potential, mainly dependent on the amino acid compositions and sequences as well as molecular weight, displayed for those of ≤20 kDa. Moreover, the antioxidant activity of sea cucumber polysaccharides, including fucosylated chondroitin sulfate and fucan, is a combination of numerous factors and is mostly associated with molecular weight, degree of sulfation, and type of major sugars. However, the activity of these bioactive compounds typically depends on the sea cucumber species, harvesting location, food habit, body part, and processing methods employed. This review summarizes the antioxidant activity of bioactive compounds obtained from sea cucumbers and their by-products for the first time. The mechanism of actions, chemical structures, and factors affecting the antioxidant activity are also discussed, along with the associated health benefits.

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Section: Bioactive Compounds Of Sea Cucumbers and Their Antioxidant A...mentioning

confidence: 99%

Antioxidant Potential of Sea Cucumbers and Their Beneficial Effects on Human Health

2022

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“…The test results showed that H. leucospilota had the best antioxidant activity of 11.12% at a dose of 1.0 mg/ml extract. Other research showed that collagen hydrolysate of S. horrens had an IC50 value of 5.25 mg/ml to DPPH free radicals [21].…”

Section: Resultsmentioning

confidence: 94%

Antioxidant capacity of dry sea cucumber Holothuria edulis, Pearsonothuria graeffei, and Stichopus herrmanni from Boalemo waters, Gorontalo, Indonesia

Nursid

Hadiati

Winanto

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Sea cucumber was a marine invertebrate from class Holothuroidea and phylum Echinoderm that possessed various bioactive compounds. This study aimed to estimate the antioxidant properties of sea cucumbers and evaluate their antioxidant ability. Ferric Reducing Antioxidant Power (FRAP) and Cupric Reducing Antioxidant Capacity (CUPRAC) assay estimated the antioxidant properties of sea cucumbers, and for control, ascorbic acid and Trolox were used. The samples were dried sea cucumber of Holothuria edulis, Pearsonothuria graeffei, and Stichopus herrmannii from Boalemo, Gorontalo. The three samples had various antioxidant capacities. H. edulis had the highest value, both in FRAP and CUPRAC assay, followed by P. graeffei and S. herrmanni. The FRAP values were 48.47; 28.32; and 11.95 μmol Fe(II), respectively and the CUPRAC values were 6.5; 5.9; and 1.49 μmol Trolox/g extract. The weak antioxidant properties of sea cucumber extract in both methods were most likely related to the absence of phenolic compounds which usually have OH groups and conjugated double bonds. Separation and purification of the crude extract may improve its antioxidant properties. Based on this research, it can be concluded that the antioxidant capacity of H. edulis, P. graeffei, and S. herrmanni using the FRAP and CUPRAC method was weak.

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“…Previous research regarding hydrolyzed collagen derived from golden sea cucumbers has an antioxidant effect (IC50 of 5.25±0.15 mg/ml) [9]. Other studies confirm that sea cucumbers are a source of collagen and need further research [10].…”

Section: Introductionmentioning

confidence: 92%

Formulation and Characterization of Serum Collagen of Sea Cucumber Extract Stichopus Horrens as an Antioxidant

et al. 2021

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Objective: The study was aimed to extract, formulate and characterize collagen extract of Stichopus horrens into serum preparations and decide antioxidant activity in powders and serum preparations. Methods: The sea cucumber meat was extracted collagen in three stages, namely the pretreatment stage using 0.3 M NaOH solution 1:10 (w/v) for 48 h, the hydrolysis step in the 0.3 M 1:10 acetic acid solution (w/v) for 48 h, and the extraction stage with distilled water 1: 2 (w/v) for 2 h at 45 °C. The collagen extract was freeze-dried to obtain collagen powder. Collagen powder was characterized by HPLC and its antioxidant activity was determined using the DPPH method. Collagen powder formulated with extract variation of 0, 0.5 and 1%. Evaluation of serum included organoleptic, homogeneity, stability of pH and viscosity as well as antioxidant activity. Results: The results showed that collagen powder had a % yield of 0.24%, which consisted of the amino acids glycine, proline, alanine, and glutamic acid as the dominant amino acids. The % Free radical inhibition of collagen powder at concentration of 5000 ppm was 63.23%. IC50 values were obtained at 4045.37 ppm. The stability test resulted in stable serum preparations without significant changes at 4 °C and 27 °C±2 °C storage temperatures. Conclusion: The measurement of DPPH Radical reduction activity in the highest serum preparation was 1% extract with a value of 2.4%.

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Golden Sea Cucumber: Identification and the Antioxidant Activity of Its Collagen Hydrolysates

Cited by 9 publications

References 40 publications

Antioxidant Potential of Sea Cucumbers and Their Beneficial Effects on Human Health

Antioxidant Potential of Sea Cucumbers and Their Beneficial Effects on Human Health

Antioxidant capacity of dry sea cucumber Holothuria edulis, Pearsonothuria graeffei, and Stichopus herrmanni from Boalemo waters, Gorontalo, Indonesia

Formulation and Characterization of Serum Collagen of Sea Cucumber Extract Stichopus Horrens as an Antioxidant

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