Bioactivity and antibacterial activity of iodine-containing calcium titanate against implant-associated infection

Ikeda, Nisaburo; Fujibayashi, Shunsuke; Yamaguchi, Seiji; Goto, Koji; Otsuki, Bungo; Kawai, Toshiyuki; Shimizu, Takayoshi; Okuzu, Yaichiro; Masamoto, Kazutaka; Shimizu, Yu; Takaoka, Yusuke; Matsuda, Shinya

doi:10.1016/j.bioadv.2022.212952

Cited by 10 publications

(14 citation statements)

References 49 publications

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“…The first part of the research was devoted to verifying the effective presence of both polyphenols and iodine ions on the functionalized surfaces and to their characterization. The Ti_Ca+I surface has already been characterized in [ 19 ] and it was here used as a reference.…”

Section: Resultsmentioning

confidence: 99%

“…Attention then shifted to iodine ions as excellent candidates for antibacterial, biocompatible, and noncytotoxic agents. When an iodine-containing calcium titanate is produced on Ti by a chemical and heat treatment, the treated metal exhibits in vitro and in vivo antibacterial activity, in vitro apatite formation, and in vivo bone bonding ability [ 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…These substrates have been extensively characterized in previous works and their osseointegration ability is already verified. In fact, a high long-term sustainable bone-bonding capacity was confirmed by in vivo tests and mechanical and histological experiments [ 19 , 22 ]. In addition, the same type of green tea used here (TPH) has been previously tested and was found to have good ability to promote osteoblast differentiation and mineralization [ 43 ].…”

Section: Introductionmentioning

confidence: 99%

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Conferring Antioxidant Activity to an Antibacterial and Bioactive Titanium Surface through the Grafting of a Natural Extract

et al. 2023

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The main unmet medical need of bone implants is multifunctional activity, including their ability to induce rapid and physiological osseointegration, counteract bacterial biofilm formation, and prevent in situ chronic inflammation at the same time. This research starts from an already developed c.p. titanium surface with proven bioactive (in vitro hydroxyl apatite precipitation) and antibacterial activities, due to a calcium titanate layer with nano- and micro-scale roughness and loaded with iodine ions. Here, antioxidant ability was added to prevent chronic inflammation by grafting polyphenols of a green tea extract onto the surface, without compromising the other functionalities of the surface. The surface was characterized before and after functionalization through XPS analysis, zeta potential titrations, ion release measurements, in vitro bioactivity tests, SEM and fluorescence microscopy, and Folin–Ciocalteu and biological tests. The presence of grafted polyphenols as a homogeneous layer was proven. The grafted polyphenols maintained their antioxidant ability and were anchored to the surface through the linking action of Ca2+ ions added to the functionalizing solution. Iodine ion release, cytocompatibility towards human mesenchymal stem cells (hMSC), and antibacterial activity were maintained even after functionalization. The antioxidant ability of the functionalized surface was effective in preserving hMSC viability in a chemically induced pro-inflammatory environment, thus showing a scavenger activity towards toxic active species responsible for inflammation.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Conferring Antioxidant Activity to an Antibacterial and Bioactive Titanium Surface through the Grafting of a Natural Extract

et al. 2023

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“…Because of their exceptional mechanical qualities, superior biocompatibility, and excellent corrosion resistance, commercially pure titanium and Ti-based alloys have been widely employed in hard tissue replacement metallic implants, especially in the orthopedic and dental professions [5][6][7][8]. Among the most recommended reasons for boneimplant failure are stress shielding, inadequate osseointegration, and a high potential for bacterial infections.…”

Section: Introductionmentioning

confidence: 99%

“…Tey are produced by wound contamination after surgery and bacterial strains introduced into the surfaces of metallic implants and surgical instruments due to insufcient sanitation. Infections caused by medical devices increase healthcare expenses, cause patient misery, and, in some difcult situations, result in death [6,16].…”

Section: Introductionmentioning

confidence: 99%

Metallic Implant Surface Activation through Electrospinning Coating of Nanocomposite Fiber for Bone Regeneration

Al-Khateeb

Al-Hassani

Jabur

2023

International Journal of Biomaterials

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There is a critical need in orthopedic and orthodontic clinics for enhanced implant-bone interface contact to facilitate the quick establishment of a strong and durable connection. Surface modification by bioactive multifunctional materials is a possible way to overcome the poor osteoconductivity and the potential infection of Ti-based implants. Ti-25Zr biometallic alloy was prepared by powder metallurgy technique and then coated by Nano-composite fiber using electrospinning. Ceramic Nanocompound (CaTiO3, BaTiO3) was used as filler material and individually added to polymeric matrices constructed from the blend of polycaprolactone/chitosan. Using optical microscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR), and wettability, respectively, the morphology, chemical analysis, surface roughness, and contact angle measurements of the samples were evaluated. The result shows a significant improvement in cell viability, proliferation, and ALP activity for coated samples compared to noncoated samples. PCL/Chitosan/Nano-CaTiO3 (CA1) recorded remarkable enhancement from the surface-coated samples, demonstrating a significantly higher cell viability value after seven days of MC3T3-E1 cell culture, reaching 271.56 ± 13.15%, and better cell differentiation with ALP activity reaching 5.61 ± 0.35 fold change for the same culture time. PCL/Chitosan/Nano-BaTiO3 (BA1) also shows significant improvement in cell viability by 181.63 ± 17.87% and has ALP activity of 3.97 ± 0.67 fold change. For coated samples, cell proliferation likewise exhibits a considerable temporal increase; the improvement reaches 237.53% for (CA1) and 125.16% for (BA1) in comparison with uncoated samples (bare Ti-25Zr). The coated samples resist bacteria in the antibacterial test compared to the noncoated samples with no inhibition zone. This behavior suggests that a Nanocomposite fiber coat containing an active ceramic Nanocompound (CaTiO3, BaTiO3) promotes cell growth and holds promise for orthodontic and orthopedic bioapplication.

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Enhancement of in vitro antibacterial activity and bioactivity of iodine‐loaded titanium by micro‐scale regulation using mixed‐acid treatment

Sawai,

Yamaguchi,

Inoue

et al. 2023

J Biomedical Materials Res

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Postoperative infection and subsequent device loss are serious complications in the use of titanium dental implants and plates for jawbone reconstruction. We have previously reported that NaOH–CaCl2–thermal–ICl3–treated titanium (NaCaThIo) has a nano‐scale surface and exhibits antibacterial activity against Staphylococcus aureus. The present study examined the surface properties of mixed‐acid treated and then iodine‐treated titanium (MA–NaCaThIo), and evaluated oral antibacterial activity and cytotoxicity compared with the results obtained with NaCaThIo. MA‐NaCaThIo formed a surface layer with a nano‐scale network structure having microscale irregularities, and both the thickness of the surface layer (1.49 ± 0.16 μm) and the average surface roughness (0.35 ± 0.03 μm) were significantly higher than those of NaCaThIo. Furthermore, MA‐NaCaThIo maintained high hydrophilicity with a contact angle of 7.5 ± 1.7° even after 4 weeks, as well as improved apatite formation, iodine ion release, and antibacterial activity against Prevotella intermedia compared to NaCaThIo. Cell culture test revealed that MA‐NaCaThIo exhibited no cytotoxicity against MG‐63 and Vero cells, while increased cell proliferation, ALP activity and mineralization of MG‐63 compared to NaCaThIo. This treated titanium is expected to be useful for the development of next‐generation titanium devices having both bone‐bonding and antibacterial properties.

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Bioactivity and antibacterial activity of iodine-containing calcium titanate against implant-associated infection

Cited by 10 publications

References 49 publications

Conferring Antioxidant Activity to an Antibacterial and Bioactive Titanium Surface through the Grafting of a Natural Extract

Conferring Antioxidant Activity to an Antibacterial and Bioactive Titanium Surface through the Grafting of a Natural Extract

Metallic Implant Surface Activation through Electrospinning Coating of Nanocomposite Fiber for Bone Regeneration

Enhancement of in vitro antibacterial activity and bioactivity of iodine‐loaded titanium by micro‐scale regulation using mixed‐acid treatment

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