Bioaktivitas Ekstrak Metanol Daun Namnam Serta Kombinasinya Dengan Madu Trigona

Abstract

Namnam (Cynometra cauliflora L) and trigona honey are two potential natural ingredients to b e developed in Indonesia. However, scientific evidence of their active compounds and b ioactivity is still rarely found, particularly the combination of these two materials. Therefore , this research aimed to explore the active ingredients of the combination of the two materials, i.e. total phenolic, flavonoids, vitamin C and βcarotene and its b ioactive capabilities such as antioxidant activity and antibacterial activity. The analysis showed the methanol extract of Nam nam leaves (EMDN) and trigona honey either in their sole form or combined form has antioxidant and antib acterial activity. The EMDN was more active to Staphylococcus aureus (23.7±3.3 mm) than to Escherichia coli, while the Trigona honey (MT) more active to Escherichia coli (32.6±4.4 mm) than Staphylococcus aureus (16.6±4.1 mm). Similarly, the combination of EMDN and MT showed higher activity to Escherichia coli (23±1.9 mm) than Staphylococcus aureus (17.6±2.6 mm). Analysis of the antioxidant activity also showed that EMDN provided the highest activity with an IC 50 of 0.0048±0.000 mg/mL), while a comb ined EMDN and MT had an IC 50 of 0.0085±0.000 mg/mL) and MT with an IC50 of 3.736±0.112 mg/mL. Moreover, this analysis also showed that sole samples of MT and EMDN have total phenolic content, flavonoids, and vitamin C that were higher than the comb ination of MT and EMDN. However, the content of β-carotene in the combined form of MT and EMDN was higher. Thus trigona honey, methanol extract of leaves Nam nam (Cynometra cauliflora L) in a single form or in a combination are potential to b e utilized and developed as a source of antioxidants and antibacterial in the form of functional food.

Inhibition of namnam (Cynometra cauliflora L.) leaves extract on the growth of Porphyromonas gingivalis

“…The results of this research are in accordance with other research which states that namnam leaves extract has an antibacterial effect, but in Staphylococcus epidermidis and Pseudomonas aeuruginosa bacteria 22 . Other researcher also stated that the methanol extract of namnam leaves has antibacterial activity, namely in Staphylococcus aureus but not in E. coli 13 . The antibacterial properties found in namnam leaves extract are tannins, flavonoids, triterpenoids, saponins and quinones 13,23,24 .…”

“…Dried leaves are mashed with a blender and sieved with an 80 mesh sieve. 13 Namnam leaves powder that has been sieved is taken 200 grams and put into a closed jar, then added 1000 ml of methanol. The maceration stage was carried out for 48 hours while stirring every eight hours using a stirring rod and then it was regenerated for 48 hours.…”

“…The maceration stage was carried out for 48 hours while stirring every eight hours using a stirring rod and then it was regenerated for 48 hours. 13,14 The filtrate was evaporated with a rotary evaporator at a temperature of 50ºC to obtain concentrated extract with a concentration of 100%. Then the extract was diluted to a concentration of 80, 60, 40 and 20%.…”

“…The dilution results were filtered using a syringe filter and inserted in the eppendorf tube. 13 The next stage is the preparation of Brain Heart Infusion Broth (BHI-B) media and Brain Heart Infusion Agar (BHI-A). After making the media, followed by making a suspension of P. gingivalis bacteria.…”

Krim ekstrak daun Lantana camara Linn. 4% stabil setelah disimpan selama 1 tahun

“…Sediaan tersebut memiliki daya sebar 5-7 cm. 14,15 Berdasar hasil uji daya sebar krim ekstrak daun L. camara Linn. 3%, 4% dan 5% setelah disimpan selama 1 tahun masih bisa digunakan akan tetapi menimbulkan rasa yang kurang nyaman karena akan terasa tebal di kulit.…”